A new distribution record, first host plant record and DNA barcoding of the Neotropical micromoth Astrotischeria karsholti Puplesis & Diskus (Lepidoptera, Tischeriidae)
Author
Vargas, Hector A.
https://orcid.org/0000-0002-5355-3157
Universidad de Tarapaca, Facultad de Ciencias Agronomicas, Departamento de Recursos Ambientales, Arica, Chile
lepvargas@gmail.com
text
Biodiversity Data Journal
2023
2023-12-19
11
115397
115397
http://dx.doi.org/10.3897/BDJ.11.e115397
journal article
http://dx.doi.org/10.3897/BDJ.11.e115397
1314-2828-11-e115397
2E699B30D20B548794BA0A623548E596
Astrotischeria karsholti Puplesis &
Diskus
, 2003
Materials
Type status:
Other material
.
Occurrence:
individualCount:
2
; sex:
male
; occurrenceID:
4FCB30DA-EF41-5959-B20D-21C560A30886
;
Taxon:
scientificName: Astrotischeria karsholti Puplesis &
Diskus
, 2003; higherClassification: Insecta; Lepidoptera; Tischeriidae; genus: Astrotischeria; specificEpithet: karsholti; scientificNameAuthorship: Puplesis &
Diskus
, 2003;
Location:
continent:
South America
; country:
Chile
; stateProvince: Arica; locality:
Azapa Valley
; decimalLatitude:
-18.52
; decimalLongitude:
-70.18
;
Identification:
identifiedBy:
Hector
A. Vargas
; identificationRemarks: Genitalia slides HAV1680, HAV1681;
Event:
samplingProtocol:
Male adults emerged
October 2022
, reared from leaf mines on Ambrosia cumanensis collected
September 2022
;
Record Level:
type: PhysicalObject; language: en; institutionID: "
Coleccion
Entomologica
de la Universidad de
Tarapaca
" (IDEA)
Type status:
Other material
.
Occurrence:
individualCount:
2
; sex:
male
; occurrenceID:
98E275BB-728F-59D6-872A-79CFAFA3B21E
;
Taxon:
scientificName: Astrotischeria karsholti Puplesis &
Diskus
, 2003; higherClassification: Insecta; Lepidoptera; Tischeriidae; genus: Astrotischeria; specificEpithet: karsholti; scientificNameAuthorship: Puplesis &
Diskus
, 2003;
Location:
continent:
South America
; country:
Chile
; stateProvince: Arica; locality:
Azapa Valley
; decimalLatitude:
-18.52
; decimalLongitude:
-70.18
;
Identification:
identifiedBy:
Hector
A. Vargas
; identificationRemarks: Genitalia slides HAV1088, HAV1405;
Event:
samplingProtocol:
Male adults emerged
November 2017
, reared from leaf mines on Ambrosia cumanensis collected
October 2017
;
Record Level:
type: PhysicalObject; language: en; institutionID: "
Coleccion
Entomologica
de la Universidad de
Tarapaca
" (IDEA)
Type status:
Other material
.
Occurrence:
individualCount:
1
; sex:
male
; occurrenceID:
831F005C-C567-5180-914C-B8D68CA49522
;
Taxon:
scientificName: Astrotischeria karsholti Puplesis &
Diskus
, 2003; higherClassification: Insecta; Lepidoptera; Tischeriidae; genus: Astrotischeria; specificEpithet: karsholti; scientificNameAuthorship: Puplesis &
Diskus
, 2003;
Location:
continent:
South America
; country:
Chile
; stateProvince: Arica; locality:
Azapa Valley
; decimalLatitude:
-18.52
; decimalLongitude:
-70.18
;
Identification:
identifiedBy:
Hector
A. Vargas
; identificationRemarks: Genitalia slide HAV119;
Event:
samplingProtocol:
Male adult emerged
April 2018
, reared from leaf mines on Ambrosia cumanensis collected
March 2018
;
Record Level:
type: PhysicalObject; language: en; institutionID: "
Coleccion
Entomologica
de la Universidad de
Tarapaca
" (IDEA)
Taxonomic identification
Five male adults emerged from the mined leaves of
A. cumanensis
collected in the Azapa Valley, all of which were identified as
A. karsholti
(Fig.
1
), based on comparisons with descriptions and figures in
Puplesis and
Diskus
(2003)
and
Stonis et al. (2018)
.
Distribution
The discovery of
A. karsholti
in the Azapa Valley represents the first record of this micromoth in Chile, expanding the previously documented distribution range nearly 900 km to the southeast (Fig.
2
).
Host plant
Ambrosia cumanensis
is the first host plant recorded for
A. karsholti
. Leaf mines of
A. karsholti
were searched for on other members of
Asteraceae
growing in the study area, but no additional hosts were found for this micromoth. The egg is deposited on the abaxial surface of the leaf and the larva penetrates the leaf through this side. New mines are visible only from the abaxial surface of the leaf, while completely developed mines are partially translucent and, thus, detectable from the two leaf sides, suggesting that the larva eats a great part of the internal tissues of the leaf. The last instar constructs a well-delimited circular cell (nidus) inside the mine for pupation. Adult emergence occurs through a slit on the margin of the nidus (Fig.
3
).
DNA barcoding
Genetic divergence of
A. karsholti
with other members of
Astrotischeria
ranged between 6 and 18.4% (K2P), with
Astrotischeria trilobata
Diskus
& Stonis, 2018 and
Astrotischeria sanjosei
Stonis &
Diskus
, 2019, respectively, while it was 9.9% with
A. chilei
, the only Chilean congeneric (Suppl. material 2). The monophyly of
Coptotriche
,
Paratischeria
and
Tischeria
was strongly supported in the ML analysis (Fig.
4
). In contrast, only 12 of the 15 analysed species of
Astrotischeria
formed a monophyletic group. The relationships in this group were poorly resolved, with the exception of
A. karsholti
+
A. trilobata
and
Astrotischeria solidagonifoliella
(Clemens, 1859) +
Astrotischeria astericola
(Braun, 1972).