Account of montane and insular speciation in some Korean megadriles (Annelida: Oligochaeta) Author Blakemore, Robert J. Author Lee, Seunghan Author Seo, Hong-Yul text Journal of Species Research 2015 2015-02-28 4 1 1 22 http://dx.doi.org/10.12651/jsr.2015.4.1.001 journal article 58532 10.12651/JSR.2015.4.1.001 a0c1a8e5-7c6d-4083-820d-02293daa5e23 2713-8615 8135064 17. Eisenia muuido Blakemore sp. nov. ( Fig. 10 ). Material. IV0000261296 (DNA HY30) H, holotype , a mature specimen from Muuido (same details as IV 000 261298) collected 15 th Sept. 2013 by RJB ; IV0000261 297 P, paratype , a subadult lacking tail; plus an immature specimen (S). Etymology. After island locality (noun in apposition). Description. Pale body, pinkish in life with distinct yellow anterior and posterior tips (coelomocyctes as in E. japonica ) found in all life stages (H, P, S). Length 100 mm (H) or 55+ mm (P) with 120 segments. Open epilobous. First dorsal pore 4/5. Clitellum weak saddle in ca. 27-32,33. TP elongate bands lateral of b setae on 28-32. Setae ab on 28-32 tumid. Spermathecal pores minute in 9/10/11 dorsally as rounded sacs (one heart-shaped). Female pores in 14 just lateal of b setae. Male pores on barely marked porophores also just lateral of b setae. Nephropores not found. Holandric: testis in seminal vesicles in 9-10 medium sized and larger in 11-12. Hearts in 7-11. Calciferous glands annular in 11 & 12. Ovaries in 12 as flattened tongues with many eggs. Intestine from 1 / 215 . Crop in 16. Gizzard in 17-18. Typhlosole not noted. Nephridial bladders sausage-shaped. Much mucous produced when handled. Remarks. From Michaelsen (1900) , the closest agreement is with Eisenia tigrina (Rosa, 1896) from Europe, however it has first dorsal pore in 3/4 and much larger male pores (14/15-15/16). Of the twenty Korean lumbricids listed in Blakemore (2014) , the geographically closest are Eisenia japonica (Michaelsen, 1892) and its sub-species (as described by Blakemore & Grygier, 2011 ; Blakemore, 2012c ; 2013) that have clitella around 24-31 and TP restricted to 27 & 29 or E. koreana (Zicsi, 1972) , E. gaga Blakemore, 2012 , and E. sindo Blakemore, 2012 all three of which differ, however, by having clitella near 25-32 and TP around 27-29. Several of these taxa are genetically tested in Appendix and show matches no closer than 79-84% thus helping to define the uniqueness of the current worm. Blakemore, R.J. 2000 . . VermEcology, PO BOX 414 Kippax 2615. Canberra, December, 2000. pp. 800 including 222 figures . Blakemore, R.J. 2002 . . VermEcology, Kippax, Australia . pp. 506 [CD publication]. Blakemore, R.J. 2003 . . Organisms, Diversity and Evolution 3 ( 3 ): 241-244 [Available from: http://www.urbanfischer.de/journals/ode/ ; www.sencken berg.de/odes/03-11.htm]. Fig. 10. Eisenia muuido sp. nov. Holotype showing spermathecae, nephridium 18rhs and calciferous glands in 11 & 12. Blakemore, R.J. 2006 . . Yokohama National University, Japan . - 3rd Edition (2008): http://www.annelida.net/earthworm/ (accessed 13/03/ 2013). DNA barcode Blast results show match no closer than 85-86% for Eisenia nordenskioldi aff. nordenskioldi (Eisen, 1879) (Genbank JX531498.1 from a Russian study) and its sub-species as redescribed by Blakemore (2013c : tab. 1) that have clitella in the region of 26,27- 32,33 and TP 29-31, thus closest to the current taxon. In Korean Allolobophora species reviewed by Blakemore (2013c : tab. 2), viz. Allolobophora hataii , A. harbinensis and A. dairenensis all by Kobayashi (1940), their clitella are in region of 23,24,25-32,33 and TP in 29-31 only, thus they too differ morphologically.