Identification of endophytic fungi from leaves of Pandanaceae based on their morphotypes and DNA sequence data from southern Thailand
Author
Tibpromma, Saowaluck
Author
Hyde, Kevin D.
Author
Bhat, Jayarama D.
Author
Mortimer, Peter E.
Author
Xu, Jianchu
Author
Promputtha, Itthayakorn
Author
Doilom, Mingkwan
Author
Yang, Jun-Bo
Author
Tang, Alvin M. C.
Author
Karunarathna, Samantha C.
text
MycoKeys
2018
33
25
67
http://dx.doi.org/10.3897/mycokeys.33.23670
journal article
http://dx.doi.org/10.3897/mycokeys.33.23670
1314-4049-33-25
Colletotrichum fructicola Prihast., L. Cai & K.D. Hyde, 2009
Culture characteristics.
Colonies
on PDA (Figure 2, PE84, 88), superficial, white to olivaceous in the beginning and later become olivaceous to dark-olivaceous, circular, entire edge, smooth, dense and raised on surface media; reverse dark-olivaceous. Sporulating in culture after 1 month.
GenBank numbers.
MFLUCC 17-0613 ITS=MG646968,
β-tubulin
=MG646927, GAPDH=MG646932, CHS-1=MG646937, ACT=MG646939. MFLUCC 17-0555 ITS=MG646969,
β-tubulin
=MG646928, GADPH=MG646933, CHS-1=MG646936, ACT=MG646944.
Notes.
The
gloeosporioides
species complex is mainly plant pathogens (
Weir et al. 2012
) and some species are endophytes (
Liu et al. 2015
).
Colletotrichum fructicola
has a wide host range (
Weir et al. 2012
) and was originally reported from coffee berries in Thailand (
Prihastuti et al. 2009
). In this study, the authors followed
Jayawardena et al. (2016)
and identify the collection as
Colletotrichum fructicola
which was isolated from a
Pandanaceae
host. Based on phylogenetic analysis, this taxon grouped with
Colletotrichum fructicola
with 90 % in ML and 1.00 in PP. The ITS,
β-tubulin
, GAPDH, CHS-1 and ACT DNA nucleotide comparison showed that the taxon and other strains of
Colletotrichum fructicola
Prihast., L. Cai & K.D. Hyde have 100% similarity.