New cave-dwelling pseudoscorpions of the genus Lagynochthonius (Pseudoscorpiones, Chthoniidae) from Guizhou in China Author Hou, Yanmeng 0000-0003-0059-3419 Author Feng, Zegang 0000-0002-5809-5984 Author Zhang, Feng 0000-0002-3347-1031 text Zootaxa 2023 2023-06-27 5309 1 1 64 http://dx.doi.org/10.11646/zootaxa.5309.1.1 journal article 55245 10.11646/zootaxa.5309.1.1 048abd7b-8c78-494e-b466-b614aeef7f6b 1175-5326 8090046 C930242A-041C-4CEC-8B2F-2DB671A2395D Lagynochthonius nigriculus sp. nov. (ḆṘṄṁae) Figs 31–35 Type material. Holotype ♂ (Ps.- MHBU-HBUARA#2021-423 - 01 ): China , Guizhou Province , Weining County , Yangjie Town , Songshan Village , Yuqiyan Cave , under stones in the deep zone [ 26°59′21.87″N , 104°20′23.68″E ], 2143 m a.s.l. , 4 October 2021 , Zegang Feng , Yanmeng Hou , Lu Zhang & Liu Fu leg. Paratypes : 4 ♀ (Ps.- MHBU-HBUARA#2021-423 - 02 –HBUARA#2021-423-05), all with the same data as the holotype ; 6 ♀ (Ps.- MSWU-HBUARA#2021-425 - 01 –HBUARA#2021-425-06), Songshan Village , unnamed cave, [ 26°59′14.67″N , 104°20′33.35″E ], 2165 m a.s.l. , with the same collection date and collectors as the holotype ; 1 ♂ (Ps.- MSWU-HBUARA#2022-495 - 01 ) , 4 ♀ (Ps.- MSWU-HBUARA#2022-495 - 02 –HBUARA#2022-495-05), Songshan Village , unnamed cave, 31 July 2022 , Yanmeng Hou , Lu Zhang , Jianzhou Sun & Wenlong Fan leg. Etymology. The specific name is derived from the Latin adjective “ nigriculus ”, meaning blackish, which refers to the black tergites. Diagnosis (♂ ♀ ). Moderately sized troglomorphic species with elongated appendages; carapace without eyes or eyespots; anterior margin of carapace thin, finely denticulated, epistome small and pointed, triangular; posterior margin of carapace with two setae; tergites Ⅰ–II each with two setae. Pedipalps slender, femur 8.93–9.00 (♂), 8.24– 8.67 ( ♀ ) times longer than broad; chela 8.00–8.14 (♂), 7.25–7.68 ( ♀ ) times longer than broad; chelal fingers without intercalary teeth but fixed chelal finger with a modified accessory tooth ( td ) on retrolateral face; chemosensory setae ( sc ) present on dorsum of chelal hand. Description. Males ( holotype and paratype ) ( Figs 31D , 33A , 34A–F , 35 ). FIGURE 31. Yuqiyan Cave, type locality of Lagynochthonius nigriculus sp. nov. , A. Entrance; B. Inside the cave entrance; C. Area where L. nigriculus sp. nov. specimens were collected; D. Live male of L. nigriculus sp. nov. in its natural environment; E. Live female of L. nigriculus sp. nov. in its natural environment. Colour: generally pale yellow, chelicerae, pedipalps and tergites slightly darker, soft parts pale. Cephalothorax ( Figs 34C , 35A ): carapace 0.97–1.05 times longer than broad, gently narrowed posteriorly; surface smooth, without furrows; no traces of eyes; anterior margin slightly serrate; epistome small and pointed, triangular, with two setae flanking base; with 18 setae arranged s4s: 4: 4: 2: 2, most setae heavy, long and gently curved, anterolateral setae much shorter than others; with two pairs of lyrifissures, first pair situated middle to the setae of ocular row, the second situated exterior to the sole pair of setae of posterior row. Chaetotaxy of coxae: P 3, Ⅰ 3, II 3–4, III 5, IV 5; manducatory process with two acuminate distal setae, anterior seta less than 1/2 length of medial seta; apex of coxa Ⅰ with small, rounded anteromedial process; coxae II with 13–15 terminally indented coxal spines on each side, set as an oblique and arc row, longer spines present in the middle of the row, becoming shorter distally and proximally and incised for about half their length ( Fig. 35C ); intercoxal tubercle absent; without sub-oral seta. Chelicera ( Figs 34D , 35B ): large, about as long as carapace, 2.43–2.48 times longer than broad; five setae and two lyrifissures (exterior condylar lyrifissure and exterior lyrifissure) present on hand, all setae acuminate, ventrobasal seta shorter than others; movable finger with one medial seta. Cheliceral palm with moderate hispid granulation on both ventral and dorsal sides. Both fingers well provided with teeth, fixed finger with 19–20 teeth, distal one largest; movable finger with 21–23 retrorse contiguous small teeth; galea absent ( Fig. 35B ). Serrula exterior with 23 blades and serrula interior with 12 blades. Rallum with eight blades, the distal one longest and recumbent basally, with fine barbules and slightly set apart from the other blades, latter tightly grouped and with long pinnae, some of which are subdivided ( Fig. 35D ). FIGURE 32. unnamed cave, type locality of Lagynochthonius nigriculus sp. nov. , A. Entrance; B. Inside the cave entrance; C–D. Areas where L. nigriculus sp. nov. specimens were collected. Pedipalp ( Figs 34A–B, E , 35E–G ): long and slender, trochanter 1.32–1.44, femur 8.93–9.00, patella 2.56–2.67, chela 8.00–8.14, hand 3.33–3.41 times longer than broad; femur 2.81–3.05 times longer than patella; movable chelal finger 1.37–1.39 times longer than hand and 0.58 times longer than chela. Setae generally long and acuminate; one distal lyrifissures present on patella ( Figs 34E , 35E ). Chelal palm gradually constricted towards fingers, apodeme complex of movable chelal finger strongly sclerotized, with weak granulation dorsally at base of fixed chelal finger and hand. Fixed chelal finger and hand with eight trichobothria, movable chelal finger with four trichobothria, ib and isb situated close together, submedially on dorsum of chelal hand; eb , esb and ist forming a straight oblique row at base of fixed chelal finger; it slightly distal to est , situated subdistally; et slightly near to tip of fixed chelal finger, very close to chelal teeth; dx situated distal to et ; sb closer to st than to b ; b and t situated subdistally, t situated distal to b ; b distal to est ; it situated between t and b ( Fig. 35F ). A tiny retrolateral lyrifissure present at base of fixed chelal finger (situated distal to ist ). Both chelal fingers with a row of teeth, homodentate, spaced regularly along the margin, larger and well-spaced teeth present in the middle of the row, becoming smaller and closer distally and proximally: fixed chelal finger with 29–30 macrodenticles, slightly retrorse and pointed, plus a modified accessory tooth on retrolateral face ( td , close to dx ), 30–31 in total; movable chelal finger with 20–21 macrodenticles (smaller than teeth on fixed chelal finger), slightly retrorse and pointed, plus 7–9 vestigial, rounded and contiguous basal teeth, 28–29 in total ( Fig. 35F ). Chelal fingers slightly curved in dorsal view; microsetae (chemosensory setae) present on dorsum of chelal hand ( Figs 34B , 35G ). Opisthosoma: generally typical, pleural membrane finely granulated. Tergites and sternites undivided; setae uniseriate and acuminate. Tergal chaetotaxy Ⅰ–XII: 2: 2: 2–4: 4: 4: 4–6: 4–5: 5: 5: 2: T2T: 0, tergites VIII and IX each with an unpaired median seta. Sternal chaetotaxy III–XII: 13–14: 11–12: 8–9: 7–8: 7–9: 8–9: 7–9: 7–8: 0: 2. Anterior genital operculum with 11 setae, genital opening slit-like, with 12–15 marginal setae on each side, 36–39 in total ( Fig. 35F ). Legs ( Fig. 35H–I ): generally typical, long and slender. Fine granulation present on anterodorsal faces of femur IV and patella IV. Femur of leg Ⅰ 1.83–1.92 times longer than patella and with one lyrifissure at the base of femur; tarsus 2.59 times longer than tibia. Femoropatella of leg IV 4.68–4.95 times longer than deep; tibia 6.90–7.00 times longer than deep; with basal tactile setae on both tarsal segments: metatarsus 3.88–4.14 times longer than deep (TS = 0.26–0.28), tarsus 14.83–15.40 times longer than deep and 2.66–2.87 times longer than metatarsus (TS = 0.34–0.36). Arolium slightly shorter than the claws, not divided; claws simple. Adult females ( paratypes ) ( Figs 31E , 33B , 34G ). Mostly same as males, but a little larger; chaetotaxy of coxae: P 3, Ⅰ 3–4, II 4, III 5, IV 5; tergal chaetotaxy Ⅰ–XII: 2: 2: 2–4: 3–4: 4: 4: 4: 5: 5: 2: T2T: 0; sternal chaetotaxy IV–XII: 12–14: 8–10: 8–10: 8–9: 8–9: 7–9: 7–8: 0: 2; anterior genital operculum with 10–12 setae, posterior margin with 13–15 marginal setae, 24–26 in total; leg IV with a long tactile seta on both tarsal segments: metatarsus 3.63–3.88 times longer than deep (TS = 0.23–0.31), tarsus 14.50–17.20 times longer than deep and 2.77–2.97 times longer than metatarsus (TS = 0.28–0.34). FIGURE 33. Lagynochthonius nigriculus sp. nov. , A. Holotype male, habitus (dorsal view); B. Paratype female, habitus (dorsal view). Dimensions (length/breadth or, in the case of the legs, length/depth in mm; ratios in parentheses). Males: body length 2.00. Pedipalps: trochanter 0.23–0.25/0.16–0.19 (1.32–1.44), femur 1.25–1.35/0.14–0.15 (8.93–9.00), patella 0.41–0.48/0.16–0.18 (2.56–2.67), chela 1.79–1.92/0.22–0.24 (8.00–8.14), hand 0.75–0.80/0.22–0.24 (3.33– 3.41), movable chelal finger length 1.03–1.11. Chelicera 0.67–0.73/0.27–0.30 (2.43–2.48), movable finger length 0.37–0.40. Carapace 0.56–0.64/0.58–0.61 (0.97–1.05). Leg Ⅰ: trochanter 0.16–0.20/0.13–0.16 (1.23–1.25), femur 0.69–0.73/0.08–0.09 (8.11–8.63), patella 0.36–0.40/0.07–0.08 (5.00–5.14), tibia 0.29–0.32/0.06–0.07 (4.57–4.83), tarsus 0.75–0.83/0.06 (12.50–13.83). Leg IV: trochanter 0.26–0.29/0.15–0.16 (1.73–1.81), femoropatella 0.94– 1.03/0.19–0.22 (4.68–4.95), tibia 0.63–0.69/0.09–0.10 (6.90–7.00), metatarsus 0.29–0.31/0.07–0.08 (3.88–4.14), tarsus 0.77–0.89/0.05–0.06 (14.83–15.40). FIGURE 34. Lagynochthonius nigriculus sp. nov. , holotype male (A–F), paratype female (G): A. Left chela (lateral view); B. Left chela (dorsal view); C. Carapace (dorsal view); D. Left chelicera (dorsal view); E. Left pedipalp (minus chela, femoral and patellar segments are disconnected, dorsal view); F. Male genital area (ventral view); G. Female genital area (ventral view). FIGURE 35. Lagynochthonius nigriculus sp. nov. , holotype male: A. Carapace (dorsal view); B. Left chelicera (dorsal view), with details of teeth; C. Coxal spines on coxae II (ventral view); D. Rallum; E. Left pedipalp (minus chela, dorsal view); F. Left chela (lateral view), with details of teeth and trichobothrial pattern; G. Left chela (dorsal view); H. Leg Ⅰ (lateral view); I. Leg IV (lateral view). Scale bars: 0.20 mm (A–B, E–I); 0.10 mm (C–D). Females: body length 2.17–2.74. Pedipalps: trochanter 0.23–0.26/0.18–0.21 (1.19–1.37), femur 1.30–1.40/0.15– 0.17 (8.24–8.67), patella 0.44–0.48/0.18–0.20 (2.40–2.67), chela 1.84–2.03/0.24–0.28 (7.25–7.68), hand 0.78– 0.84/0.24–0.28 (3.00–3.25), movable chelal finger length 1.06–1.20. Chelicera 0.73–0.81/0.29–0.33 (2.38–2.52), movable finger length 0.40–0.44. Carapace 0.61–0.72/0.64–0.68 (0.93–1.06). Leg Ⅰ: trochanter 0.18–0.22/0.15– 0.18 (1.19–1.25), femur 0.71–0.78/0.08–0.10 (7.80–9.13), patella 0.37–0.42/0.08–0.10 (4.67–5.29), tibia 0.30– 0.34/0.06–0.07 (4.86–5.33), tarsus 0.77–0.89/0.06–0.07 (12.71–13.33). Leg IV: trochanter 0.27–0.31/0.15–0.17 (1.71–1.94), femoropatella 1.00–1.10/0.20–0.22 (4.59–5.00), tibia 0.65–0.71/0.10–0.11 (6.18–6.50), metatarsus 0.29–0.34/0.08–0.09 (3.63–3.88), tarsus 0.83–0.95/0.05–0.06 (14.50–17.20). Distribution. Known only from the Yuqiyan and unnamed caves.