Phytochemical profiles of the leaves of Stizolophus balsamita and Psephellus sibiricus and their chemotaxonomic implications Author Nawrot, Joanna ∗ & Department of Medicinal and Cosmetic Natural Products, Poznan University of Medical Sciences, Mazowiecka Str. 33, 60 - 623 Poznan, Poland Author Budzianowski, Jaromir Author Nowak, Gerard text Phytochemistry 2019 2019-03-31 159 172 178 http://dx.doi.org/10.1016/j.phytochem.2018.12.022 journal article 10.1016/j.phytochem.2018.12.022 1873-3700 10481924 3.4. Extraction and isolation of compounds from P. sibiricus leaves The dried leaves of P. sibiricus ( 453 g ) were crushed and soaked in MeOH. The MeOH extract was evapd, and the residue was dissolved in H 2 O. The aq. soln was re-extracted with CH 2 Cl 2 . The CH 2 Cl 2 extract was dried with Na 2 SO 4 , filtrated and evapd gave a residue ( 10.9 g ) and chromatographed with CC method on silica gel with a mixtures of CH 2 Cl 2 and ( CH 3 ) 2 CO (ratio 45:1, 35:1 and 10:1) as eluent. The result was the isolation of the following compounds: scoparone ( 9 ) ( 12.7 mg , amorphous solid), scopoletin ( 10 ) ( 45.4 mg , m.p. 192–195 °C), umbelliferone ( 11 ) ( 63.7 mg , m.p. 230 °C), cynaropicrin ( 12 ) ( 6.7 mg , amorphous solid) and desacylcynaropicrin 8α-( Z )-(4′-hydroxy-2′-methyl) butenoate (]cebellin F) ( 13 ) ( 3.9 mg , amorphous solid). NMR data in Tables 7 and 8 .