Revision of Chlorionidea Löw (Hemiptera: Delphacidae) with the description of two new species from Italy, and comments on anti-symmetry in male genitalia of Delphacidae Author Guglielmino, Adalgisa Author Bückle, Christoph text Journal of Natural History 2010 2010-11-05 44 45 - 46 2737 2759 http://dx.doi.org/10.1080/00222933.2010.512399 journal article 10.1080/00222933.2010.512399 1464-5262 5212071 Chlorionidea flava Löw Redescription General colour white or, more often, distinctly yellow. In this case a middle line from vertex until abdomen tip remains white. Wings hyaline except for their white margin. Dark brown markings only along white carinae of anterior part of vertex and of frons. Area between dark markings of frons orange. Figure 2. Scheme of genital apparatus. (A) Chlorionidea apenninica sp. nov. ; (B) Chlorionidea bromi Emeljanov ; (C) Chlorionidea sibillinica sp. nov. Vertex with nearly parallel or apically slightly converging lateral margins, longer than wide, its apical margin distinctly protruding in the middle. Basal cells longer than wide at their base. Apical cell very narrow, longer than basal cells, its lateral carinae fusing at tip of vertex. Transition vertex – frons in lateral view subangular. Frons in lateral view slightly convex, lateral carina almost straight, its transition to lateral carina of vertex subacutely angular. Frons in frontal view with equally curved lateral margins, in the middle with distinct simple carina continuing on postclypeus. Antennae cylindrical, with basal segment short, broader than long, second segment about three times as long as basal segment, twice as long as broad. Pronotum about three-quarters as long as vertex, its lateral dorsal carinae distinctly diverging, directed to posterior margin without attaining it. Mesonotum in the middle line twice as long as pronotum. Wings (brachypterous) with hind margin widely rounded, without trace of angles, about 1.8 × longer than wide. Male genitalia. Pygofer ( Figure 4A,E ) in lateral view with almost straight posterior margin and slight angle above inferior third of hind margin; in posterior view somewhat higher than broad, distinctly broadened on the level of diaphragm with margins strongly protruding in caudal direction on both sides of diaphragm; diaphragm along ventral margin of aedeagus opening with Y-shaped sclerotized thickening, at the end of each of its arms with tubercle; aedeagus opening pentagonal, foramen of styles almost as broad as aedeagus opening. Anal tube ( Figure 5A,F ) simple, without processes, ventrocaudal margin only slightly excavated. Styles ( Figure 6B ) stout, with axe-shaped apex, mediobasal portion broadened and scabrous. Suspensorium very uniformly thin on all sides. Aedeagus ( Figure 7A–G ) laterally depressed, ventrally bent, approximately uniformly wide in lateral view, apically only slightly enlarged, in most cases with phallotreme on its convex right side and curved series of about seven teeth ventrally of phallotreme. On its concave left side dorsoapically with group of about five large teeth and on ventral margin another series of teeth extending over apical half of aedeagus and directed to its left side. Length of aedeagus, shape of apex and shape and number of teeth variable. Remarks Anti-symmetry is observed. Some specimens have an aedeagus concave on the right side with phallotreme on the left side etc. ( Figure 7D–G ). Both types may be found together in the same population (for example in our material from Berchtesgaden, Germany ). Female genitalia. Genital scale ( Figure 9A ) large, longer than wide, to some degree variable in shape, approximately triangular with concave sides, and tapered, narrowly rounded, tip. Gonocoxae VIII wide, at most slightly concave on their median margin. Measurements Males (brachypterous). Total length: 2.8–3.3 mm ; width at level of wings: 1.1– 1.24 mm ; width of head including eyes: 0.70–0.79 mm ; length of vertex: 0.33–0.37 mm ; width of vertex at base: 0.27–0.3 mm ; length of pronotum: 0.22–0.25 mm ; length of mesonotum from hind margin of pronotum to tip of scutellum: 0.42–0.54 mm ; length of forewing: 1.0– 1.26 mm ; width of forewing: 0.69–0.8 mm ; length of hind tibia: 0.75–0.82 mm ; length of aedeagus: 0.54–0.65 mm . Males ( macropterous ): total length: Figure 3. (A, B) Chlorionidea apenninica sp. nov. , male (paratype: Italy, Emilia Romagna, Sestola): (A) head, pronotum, scutellum from above; (B) head and pronotum from the left. (C, D) Chlorionidea sibillinica sp. nov. , male (paratype: Italy, Marche, Forca di Presta): (C) head, pronotum, scutellum from above; (D) head and pronotum from the left. Figure 4. Pygofer: (A, E) Chlorionidea flava (Löw) (Germany, Bavaria, Benninger Ried); (B, F) Chlorionidea apenninica sp. nov. (paratype: Italy, Emilia Romagna, Sestola); (C, G) Chlorionidea bromi Emeljanov (Russia, Caucasus, Krasnodar, Alous Mountains); (D, H, I) Chlorionidea sibillinica sp. nov. (paratype: Italy, Marche, Forca di Presta). Views are: (A–D) from behind; (E–H) from the right; (I) laterocaudal. Figure 5. Anal tube: (A, F) Chlorionidea flava (Löw) (Germany, Bavaria, Benninger Ried); (B, G) Chlorionidea apenninica sp. nov. (paratype: Italy, Emilia Romagna, Sestola); (C, H) Chlorionidea bromi Emeljanov (Russia, Caucasus, Krasnodar, Alous Mountains); (D, I) Chlorionidea bromi Emeljanov (Ukraine, Crimea, Chatyrdag); (E, K) Chlorionidea sibillinica sp. nov. (paratype: Italy, Marche, Forca di Presta). Views are: (A–E) from behind; (F–K) from the left. 4.2 mm ; length mesonotum: 0.65 mm ; length of forewing: 0.34 mm ; width of forewing at level of apex of clavus: 0.94 mm . Females (brachypterous). Total length: 3.24–3.4 mm ; width at level of the wings: 1.26– 1.33 mm ; width of head including eyes: 0.78–0.82 mm ; length of vertex: 0.32–0.37 mm ; width of vertex at base: 0.28–0.31 mm ; length of pronotum: 0.24–0.28 mm ; length of mesonotum from hind margin of pronotum to tip of scutellum: 0.44–0.49 mm ; length of forewing: 1.24–1.3 mm ; width of forewing: 0.77–0.79 mm ; length of hind tibia: 0.8–0.9 mm . Figure 6. Fifth instar nymph: (A) Chlorionidea sibillinica sp. nov. (Italy, Marche, Forca di Presta). Left genital style: (B) Chlorionidea flava (Löw) (Germany, Bavaria, Benninger Ried); (C) Chlorionidea apenninica sp. nov. (paratype: Italy, Emilia Romagna, Sestola); (D) Chlorionidea bromi Emeljanov (Russia, Caucasus, Krasnodar, Alous Mountains); (E) Chlorionidea sibillinica sp. nov. (paratype: Italy, Marche, Forca di Presta). Suspensorium: (F) Chlorionidea apenninica sp. nov. (paratype: Italy, Emilia Romagna, Sestola); (G) Chlorionidea sibillinica sp. nov. (paratype: Italy, Marche, Forca di Presta). Figure 7. Aedeagus: Chlorionidea flava (Löw) (A–C) specimen from Germany, Bavaria, Benninger Ried; (D–G) specimen from Russia, Caucasus, Krasnodar, Tuapse. Views are (A, D) from the left; (B, E) from the right; (C, F) apical part, dorsal view; (G) apical part, dorsolateral view. Figure 8. Aedeagus: (A–D) Chlorionidea apenninica sp. nov. (paratype: Italy, Emilia Romagna, Sestola); (E–G) Chlorionidea bromi Emeljanov (Russia, Caucasus, Krasnodar, Alous Mountains); (H–K) Chlorionidea sibillinica sp. nov. (paratype: Italy, Marche, Forca di Presta). Views are (A, E, H) from the left; (B, F, I) from the right; (C) apical part, dorsal view; (G) ventral view; (K) apical part, ventral view; (D) apical part, dorsolateral view. Material examined Germany . Bayern , Memmingen , Benninger Ried , 600 m : 30 June 2001 , 7 males , 15 females ; same location, 1 July 2001 , 1 male , 2 females ; same location, 22 June 2002 , 5 males , 4 females ; same location, 29 July 2002 , 4 males , 2 females . Bayern , Berchtesgaden , Ramsau , 22 June 2003 , 7 males , 2 females ; same location, 19/ 20 July 2003 , 6 males , 1 female ; same location, 4 September 2003 , 1 male ( CB ) . Figure 9. Female: (A) Chlorionidea flava (Löw) (Germany, Bavaria, Benninger Ried); (B, C) Chlorionidea apenninica sp. nov. (paratype: Italy, Emilia Romagna, Sestola); (D) Chlorionidea bromi Emeljanov (Mongolia, Dzabkhansky Aimak, Bayan-Ula); (E, F) Chlorionidea sibillinica sp. nov. (paratype: Italy, Marche, Forca di Presta). Views are (A, B, E) genital scale; (C, D, F) abdomen from below. Russia . Krasnodar region , Tuapse district , Nebug village , forest’s edge on mountain ridge, 22 May 1965 , Logvinenko det., 1 male , 1 female ( ZIN ) . Greece . Vourinos Mountain , 1400–1600 m , 29 July 1984 , 1 male , 1 female ( CD ) .