Colletotrichum paridis sp. nov., a novel endophytic species on Paris polyphylla var. chinensis Author Cui, Hua-Wei 0009-0005-7577-4750 State Key Laboratory of Herbage Improvement and Grassland Agro-ecosystems; Center for Grassland Microbiome; Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs; Engineering Research Center of Grassland Industry, Ministry of Education; Gansu Tech Innovation Center of Western China Grassland Industry; College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China. 220220902421@lzu.edu.cn Author Malik, Kamran 0000-0003-3170-0483 State Key Laboratory of Herbage Improvement and Grassland Agro-ecosystems; Center for Grassland Microbiome; Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs; Engineering Research Center of Grassland Industry, Ministry of Education; Gansu Tech Innovation Center of Western China Grassland Industry; College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China. malik@lzu.edu.cn Author Xue, Long-Hai 0000-0002-1158-2152 State Key Laboratory of Herbage Improvement and Grassland Agro-ecosystems; Center for Grassland Microbiome; Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs; Engineering Research Center of Grassland Industry, Ministry of Education; Gansu Tech Innovation Center of Western China Grassland Industry; College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China. xuelonghai55@126.com Author Wei, Xue-Kai 0000-0002-0784-0083 State Key Laboratory of Herbage Improvement and Grassland Agro-ecosystems; Center for Grassland Microbiome; Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs; Engineering Research Center of Grassland Industry, Ministry of Education; Gansu Tech Innovation Center of Western China Grassland Industry; College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China. weixk@lzu.edu.cn text Phytotaxa 2024 2024-06-18 653 2 175 187 http://dx.doi.org/10.11646/phytotaxa.653.2.6 journal article 10.11646/phytotaxa.653.2.6 1179-3163 13216961 Colletotrichum paridis L.H. Xue, H.W. Cui & X.K. Wei , sp. nov . Figs 1d,e , 3 . Mycobank no. MB 850803 Type :— CHINA . Sichuan Province : Beichuan Qiang Autonomous County ; 31.8494°N , 104.3986°E , 1135m elevation, isolated from leaf lesions with grey mould of cultivated Paris polyphylla var. chinensis , 2017, L . H . Xue. Holotype GMCC000018 , stored in a metabolically inactive state; ex-type living culture BCTJB1 (= GMCC000018 , CGMCC 3.19921 , ACCC 35519 ). GenBank: MF775292 ( ITS ); MF775312 ( gapdh ); MF775300 ( act ); MF775304 ( tub2 ); MF775316 ( chs-1 ); MF775320 ( his3 ). Etymology:— Named after the host genus from which it was isolated, Paris . Diagnosis:— Phylogenetically, C. paridis differs (by 6 bp or more) in gapdh sequence from other species within the C. dematium complex. Description:— Endophytic on leaves of P. polyphylla var. chinensis . Asexual morph on PDA. Setae dark brown to black, smooth-walled, cylindrical to conical, tip acute or rounded, 1–4-septate, 51–193 μm (mean±SD=110±35 μm) long. Conidiophores hyaline to light brown, smooth-walled, septate. Conidia solitary, hyaline, curved or falcate, apex narrow and acute, base subtruncate, with many guttules, 21.7–31.7 × 3.7–5.5 μm (mean±SD = 24.9±2.3 × 4.6±0.5 μm; n = 50), length/width (L/W) ratio=5.4. Appressoria light brown, smooth-walled, ellipsoidal to irregular, sometimes slightly lobed, 6.2–13.5 × 4.7–11.1 μm (mean±SD = 10.2±1.7 × 6.5±1.7 μm; n = 26), length/width (L/W) ratio = 1.6. Sexual morph: undetermined. Asexual morph on SNA. Conidia solitary, hyaline, curved, apex acute, base subtruncate, with many guttules, 24.3–30.8 × 4.2–5.7 μm (mean±SD = 27.5±1.6 × 4.7±0.3 μm; n = 50), length/width (L/W) ratio = 5.9. Colonies on PDA flat with entire margin, aerial mycelium dense, cottony, surface reddish-brown to black with white to pink margin; reverse black with white to pink margin; mycelial growth rate 5.8–6.0 mm/d. Additional material examined:— CHINA . Sichuan Province : Beichuan Qiang Autonomous County, 31.8494°N , 104.3986°E , 1135m elevation, from grey mould of P. polyphylla var. chinensis , 2017, L.H. Xue, living cultures BCTJB2 (ITS accession no. MF775293), BCTJB3 ((ITS: MF775294), and BCTJB4 (ITS: MF775295). Notes : — Phylogenetically, this species is a sister group to both C. jinshuiense and Colletotrichum sp. isolate CGMCC 3.15172, but it differs at 9–18 positions from reported isolates of these two related species ( Table 2 ). Morphologically, C. paridis produces larger conidia on SNA than those of C. jinshuiense (av. 27.5 × 4.7 vs. 24 × 4 µm) ( Lee & Jung 2018 ). In addition, cultures of C. paridis on PDA show a noticeable pink ring, and have slightly faster mycelial growth rate (5.8–6 vs. 3.3–5.8 mm /d) ( Table 3 ). TABLE 2. Different nucleotide bases between Colletotrichum paridis , C. jinshuiense and Colletotrichum sp. strain CGMCC 3.15172 at different gene loci.

Base (bp) Species	Strain	ITS	act	gapdh	tub2	chs-1	his3	Summary	Literature cited
C. paridis	BCTJB1*	Check	Check	Check	Check	Check	Check	Check	This study
C. paridis	BCTJB2	0	0	0	0	0	0	0	This study
C. paridis	BCTJB3	0	0	0	0	1	0	1	This study
C. paridis	BCTJB4	0	0	0	0	1	0	1	This study
C. jinshuiense	PAFQ26*	0	2 (2gaps)	6	0	3	…	11	( Fu et al. 2019a )
C. jinshuiense	PAFQ26a	0	1 (1gap)	6	0	3 (1gap)	…	10	( Fu et al. 2019a )
C. jinshuiense	PAFQ26b	0	1 (1gap)	6	0	3 (1gap)	…	10	( Fu et al. 2019a )
C. jinshuiense	PAFQ26c	0	…	6	0	3 (1gap)	…	9	( Fu et al. 2019a )
C. jinshuiense	PAFQ26d	0	1 (1gap)	6	0	3	…	10	( Fu et al. 2019a )
C. jinshuiense	KCTC 46679*	0	0	8 (1gap)	4	4	2	18	( Lee & Jung 2018 )
C. jinshuiense	KCTC 46680	0	0	8 (1gap)	4	4	2	18	( Lee & Jung 2018 )
C. jinshuiense	LC8509	0	0	7	3	4	2	16	( Liu et al. 2022 )
C. jinshuiense	EsH8	0	0	8	3	1	…	12	( Wang et al. 2023 )
C. jinshuiense	EsH11	1 (1gap)	0	7	2	1	…	11	( Wang et al. 2023 )
Colletotrichum sp.	CGMCC 3.15172	0	0	9 (2gaps)	4 (1gap)	…	…	13	( Tao et al. 2013 )

Note: Sequences generated in this study are indicated in bold; ex-type strains are marked with an asterisk (*); …, absent. TABLE 3. Morphological characteristics of Colletotrichum paridis compared with C. jinshuiense .

Colony characters on PDA		Conidia	Appressoria		Setae
Species	Strain (Host)	Colonial morphology	Growth rate (mm/d)	Size (μm); L/W ratio	Shape	Size (μm); L/W ratio	Shape	Septate	Length (μm)
C. jinshuiense	PAFQ26* PAFQ26a-d ( Pyrus pyrifolia )	Surface pale grey-black with white margin; reverse black to dark grey-green in center with white margin	28 ℃: 5.6–5.7	on host: 24.4–27.1 × 4; 6.1–6.8	Curved, apex acute	10.7 × 6; 1.8	Ellipsoidal to clavate	1–4	59–363
C. jinshuiense (= C. kakivorum )	KCTC 46679*, KCTC 46680 (Diospyrus kaki)	Surface grey- olivaceous; reverse olivaceous-grey with smoke- grey concentric rings	25 ℃: 3.3–3.5	on SNA: 24 × 4; 6.0	Curved, apex acute	10.9 × 4.7; 2.3	Clavate or ellipsoidal to slightly lobed	3–6	77–125
C. jinshuiense	EsH8, EsH11 ( Coptis chinensis )	Surface white to pale grey; reverse yellowish to olive	25 ℃: 5.6–5.8	on host: 25.4 × 4.5; 5.6	Curved, apex acute	8.3 × 7.6; 1.1	Globose or obovoid	1–3	78–134
C. paridis	BCTJB1*, BCTJB2–4 ( Paris polyphylla )	Surface reddish-brown to black with a noticeable pink ring; reverse black with white to pink margin	25 ℃: 5.8–6	on PDA: 24.9 × 4.6; 5.4 on SNA: 27.5 × 4.7; 5.9	Curved, apex acute	10.2 × 6.5; 1.6	Ellipsoidal to irregular	1–4	51–193

Note: The new species in this study are indicated in bold; ex-type strains are marked with an asterisk (*).