Review of archaic nymphomyiids (Diptera, Nymphomyiidae) of the Russian Far East and bordering territories, with describing of new taxa and DNA barcoding of known species Author Makarchenko, Eugenyi A. Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far East Branch of the Russian Academy of Sciences, 100 let Vladivostoku 159, 690022 Vladivostok, Russia Author Semenchenko, Alexander A. Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far East Branch of the Russian Academy of Sciences, 100 let Vladivostoku 159, 690022 Vladivostok, Russia Author Krasheninnikov, Andrey B. Institute of Biological Problems of the North, Far East Branch of the Russian Academy of Sciences, Portovaya Str. 18, 685000 Magadan, Russia Author Yanygina, Lubov V. Institute for Water and Environmental Problems, Siberian Branch of the Russian Academy of Sciences, Molodezhnaya Str. 1, 656038 Barnaul, Russia Author Yavorskaya, Nadezhda M. Institute of Water and Ecology Problems, Far East Branch of the Russian Academy of Sciences, Dikopoltsev Str. 56, 680000 Khabarovsk, Russia text Zootaxa 2024 2024-05-10 5448 2 183 211 http://dx.doi.org/10.11646/zootaxa.5448.2.2 journal article 10.11646/zootaxa.5448.2.2 1175-5326 11231452 7D34476D-E6A6-4F3C-BFEB-CE0AF863FE66 Nymphomyia orientalis makcha Makarchenko et Semenchenko , subsp. nov. ( Figs. 12, 14 ) Type material. Holotype : adult male, RUSSIA : Amur Region , Zeiskyi District , environs of Zeya Town , Makcha River , 53.685022 N 127.064882 E , 29.VI.2016 , leg. N. Yavorskaya and E. Makarchenko. Paratypes : 18 adult males, 21 females , the same data as holotype, 29.VI.2016 , leg. N. Yavorskaya and E. Makarchenko. Derivatio nominis. The subspecies is named after the Makcha River, from which the type material was collected. The name is a noun in apposition. Ecology and biology. Larvae and pupae of N. orientalis makcha subsp. nov. were collected in the Makcha River ( Fig. 47 ) on the gravel-pebble soil of a riffle, at a depth of 10–20 cm , at a water temperature of 12.9 ºС and pH 7.5–7.7. Numbers of larvae reached 48 ind./m 2 in July 1, 2016 , and 144 ind./m 2 for pupae. Along with pupae, wingless adults were found in the samples, which confirms the emergence of nymphomyids already in June when the water in the river warms up to 12 ºС. Adult swarming on this river was observed on June 29, 2016 ( Yavorskaya & Makarchenko 2015 ). Distribution. N. orientalis makcha subsp. nov. is known from type locality in Makcha River of Zeya River basin only ( Fig. 2 ).