Resolving Tiarosporella spp. allied to Botryosphaeriaceae and Phacidiaceae Author Crous, Pedro W. CBS-KNAW Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands; & Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria 0002, South Africa & Microbiology, Department of Biology, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands Author Müller, Michael M. Natural Resources Institute Finland (LUKE), P. O. Box 18, FIN- 01301 Vantaa, Finland Author Sánchez, Romina M. Centro de Recursos Naturales Renovables de la Zona Semiárida-Universidad Nacional del Sur Camino La Carrindanga Km 7, B 8000 FWB, Bahía Blanca, Argentina Author Giordano, Lucrecia Centro de Recursos Naturales Renovables de la Zona Semiárida-Universidad Nacional del Sur Camino La Carrindanga Km 7, B 8000 FWB, Bahía Blanca, Argentina Author Bianchinotti, M. Virginia Centro de Recursos Naturales Renovables de la Zona Semiárida-Universidad Nacional del Sur Camino La Carrindanga Km 7, B 8000 FWB, Bahía Blanca, Argentina Author Anderson, Freda E. Centro de Recursos Naturales Renovables de la Zona Semiárida-Universidad Nacional del Sur Camino La Carrindanga Km 7, B 8000 FWB, Bahía Blanca, Argentina Author Groenewald, Johannes Z. CBS-KNAW Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands; text Phytotaxa 2015 2015-03-12 202 2 73 93 http://dx.doi.org/10.11646/phytotaxa.202.2.1 journal article 10.11646/phytotaxa.202.2.1 1179-3163 13641335 Darkera picea Crous & M.M. Müller , sp. nov. MycoBank MB811246; Fig. 5 Etymology:— Named after the host genus from which it was collected, Picea . Conidiomata globose, immersed to erumpent, brown, up to 250 μm diam, opening by means of an irregular rupture; wall of 3–6 layers of brown textura angularis . Conidiophores reduced to conidiogenous cells. Conidiogenous cells lining the inner cavity, hyaline, smooth, ampulliform to subcylindrical, proliferating percurrently at apex, mono- to polyphialidic, 5–20 × 4–5 μm. Paraphyses intermingled among conidiogenous cells, hyaline to pale brown, smooth to verruculose, 0–2-septate, subcylindrical with obtuse ends, 30–50 × 3–4 μm. Conidia solitary, hyaline, smooth, guttulate, subcylindrical to fusoid-ellipsoid, straight to curved, apex apiculate, tapering at base to truncate hilum, 2–4 μm diam, (36–)40–46(–53) × (7–)8–9(–10) μm; apex with flared mucoid appendage, up to 25 μm long, 20 μm diam (based on CPC 23897). FIGURE 5. Darkera picea (CPC 23879). A. Conidiomata on OA. B–F. Conidiogenous cells. G–J. Conidia. Scale bars: A = 250 μm, all others = 10 μm. Culture characteristics:— Colonies dirty white on all media, with moderate aerial mycelium and feathery margins, covering dish in 1 mo. Specimens examined:— FINLAND . Tuusula , Ruotsinkylä , on needles of Picea abies , 10 Sep. 1995 , A .- M . Hallaksela , Tp D 1 = CPC 23888 ; Tp T3 = 23890, Tp Q1 = 23892 ; 1994, H . Solheim , 89-2089-18 = CPC 23895 ; 1994, H . Solheim , ( holotype CBS H-21852, culture ex-type 89-2090-16 = CPC 23897 = CBS 138576 ) ; Apr. 2008 , M . M . Müller , He 392 = CPC 23900 , He 394 = CPC 23901 , He 397 = 23902 . NORWAY . Mellesmo , Pasvik , on needles of P. abies , 1994, H . Solheim , 87-1491-1 = CPC 23879 , 87-1491 - 2 = CPC 23880 ; Langtjern , 91-727-28 = CPC 23881 ; Nedstrand , 92-625-69 = CPC 23882 . SWITZERLAND , Lägern , on needles of P. abies , 30 Sep. 1986 , T . Sieber , 90.140 = CPC 23883 ; Fiesch , 90.148 = CPC 23884 ; Lägern , 90.154 = CPC 23885 l ; Davos , 90.155 = CPC 23886 ; 90.157 = CPC 23887 .