New data on taxonomy and distribution of Kaluginia lebetiformis Makarchenko, 1987 (Diptera: Chironomidae, Diamesinae) from East Asia Author Makarchenko, E. A. Author Rumyantseva, A. Yu. Author Yavorskaya, N. M. text Far Eastern Entomologist 2020 2020-01-21 399 19 28 http://dx.doi.org/10.25221/fee.399.3 journal article 10.25221/fee.399.3 2713-2196 7165439 Kaluginia lebetiformis koreana Makarchenko , subsp. n. http://zoobank.org/NomenclaturalActs/ 3D03C35E-24AF-4831-BAAB-6C5029D563E4 Figs 4–5 , 9 Kaluginia lebetiformis Makarchenko, 1987 ; Makarchenko et al ., 2018: 28 (partly), Figs 1–3, 5–10 . MATERIAL EXAMINED. Holotype : 1♂ , South Korea : Gyeongi-do , Gapyeong-gun , Gapyeongcheon, Bukhan River, Han River basin, 15. IV 2016 (Light trap), 37°58’ N , 36.4” 127°26’35.5” E , leg. Y. Bae. Paratypes : 1♂ , the same data as holotype, leg. Y. Bae ; 1♂ , the same data as holotype, except 22.V 2014 ( Light trap ), leg. H. Kang. DESCRIPTION. Male imago (n=3). Total length 2.3–2.6 mm . Total length/wing length 1.03–1.10. Total coloration brown to dark-brown; antepronotum light yellow; methonotum yellowish, with brown stripes; legs spotted: basal 2/3 of femur yellowish, distal 1/3 brown; tibia in basal and apical parts brown and yellowish in middle part; basal 2/3 of ta 1 yellowish and distal 1/3 brown; ta 2 –ta 5 brown (Makarchenko et al ., 2018, Fig. 1 ). Head . Temporal setae (from one side) including 3–4 frontals, 7 orbitals and 7–10 verti- cals. Clypeus with 17–26 setae. Palpomere length (μm): 28–48, 60–64, 104–120, 132–140, 212–220. Head width/palpal length 0.92–1.0. Antenna with 7 flagellomeres and reduced plume of setae 48–68 μm long (Makarchenko et al ., 2018, Fig. 2 ). Flagellomeres length (μm): 52–56, 24, 28, 24–28, 28–32, 28–32, 68–84; terminal flagellomere with 2 subapical setae 60–76 μm long; scape with 3 setae 28–36 μm long. AR 0.34–0.46. centrals 21–27 (72–100 μm), prealars 16, supraalars 1, scutellars ac 50. Wing . Length 2.08–2.52 mm, width 0.68–0.76 mm. Anal lobe well developed; squama with 19–31 setae (64–80 μm) in 1–2 rows. R and R 1 with 55–58 setae (32–36 μm), R 4+5 with 24–29 setae (28–40 μm) in subapical part. Wing of one male with 3 short setae on M 3+4 . Legs . BR 1 2.0; BR 2 1.8; BR 3 1.9. Spur of front tibia 44 μm long. Spurs of middle tibia 28–52 and 44–53 μm long. Spurs of hind tibia 68–72 μm and 44–52 μm long. Hind tibial comb with 12–14 spine-like setae. Middle legs with 4 pseudospurs 36–40 μm long on ta 1 ; hind legs with 7 pseudospurs on ta 1 . Claw with 4 denticles apically. Length (μm) and proportions of legs segments are as follow:

P	fe	ti	ta 1	ta 2	ta 3	ta 4	ta 5	LR	BV	SV
P 1	918– 1115	1132– 1328	722– 886	394– 459	197– 213	66–74	98– 115	0.64– 0.67	3.67– 3.87	2.76– 2.84
P 2	984– 1197	1082– 1246	574– 738	312– 394	189– 213	74– 115	115	0.53– 0.59	3.61– 4.0	3.31– 3.60
P 3	1000– 1214	1230– 1443	705– 886	394– 508	213– 246	66–74	98– 131	0.57– 0.61	3.69– 3.81	3.0– 3.16

Hypopygium ( Figs 4–5 , 9 ). Tergite IX with anal point which like small rounded protuberance, and with 29–42 setae, 12–20 μm long; anal-lateral corners not or slightly elongated; laterosternite IX with 15–20 setae (from one side), 44–48 μm long. Transverse sternapodeme high, almost trapezoidal, 128–132 μm long and 148–152 μm wide in basal part, 88–100 μm wide in subapical part, with rounded apex. Gonocoxite 308–310μm long. Basal lobe of gonocoxite is various shapes and depends on its position; in the inner half with microtrichia, along the outer margin with short setae; inferior volsella as in Fig. 5 , covered by setae 40–60 μm long. Gonostylus 156 μm long, scoop-shaped; inner lobe along the margin with 5–9 megasetae 12–16 μm long and one subterminal tooth; outer lobe widely triangular, inner margin of which with strong setae 20–24 μm long, outer half with thinner and longer setae 56–72 μm long. HR 1.97. DIAGNOSTIC CHARACTERS. The adult male of K. lebetiformis koreana subsp. n. is separated from K. lebetiformis lebetiformis mainly in the features of hypopygium, namely anal point like small rounded protuberance, anal-lateral corners of tergite IX not or slightly elongated ( Fig. 9 ), while male of K. lebetiformis lebetiformis with round triangular dark anal point and anal lateral corners of tergite IX elongated as narrow lobes to back ( Figs 6–8 ). A more detailed comparative characteristic of these subspecies is given in Table 1 , as well as in the results of DNA barcoding ( Fig. 10 ). DISTRIBUTION. This subspecies is known only from South Korea . ETYMOLOGY. The subspecies is named as koreana after the type locality in South Korea.