Deinococcus hibisci sp. nov., isolated from rhizosphere of Hibiscus syriacus L. (mugunghwa flower) Author Moya, Gabriela Author Yan, Zheng-Fei Author Chu, Dong-Hun Author Won, KyungHwa Author Yang, Jung-Eun Author Wang, Qi-Jun Author Kook, Moo-Chang Author Yi, Tae-Hoo text International Journal of Systematic and Evolutionary Microbiology 2018 2018-01-01 68 1 28 34 http://dx.doi.org/10.1099/ijsem.0.002405 journal article 20683 10.1099/ijsem.0.002405 76e0a39f-12c8-4a82-8c0b-7a3ce1d95a60 1466-5034 6048406 DESCRIPTION OF DEINOCOCCUS HIBISCI SP. NOV. Deinococcus hibisci (hi.bis′ ci. L. gen. n. hibisci of the plant genus Hibiscus ). Cells are Gram-positive cocci, 2.7–1.9×1.3–0.9 µm, strictly aerobic and non-motile. Colonies are smooth, bright, flat, circular, pink-coloured and 0.5–0.8 mm in diameter. Catalase and oxidase activities are positive. Flexirubin-type pigments are not produced. Can grow in TSB at 15–38 Ǫ C, optimum growth occurs from 25–30 Ǫ C. Growth occurs on R2A, TSA and NA agar, grows weakly on LA and MacConkey agar but not on MA. Strain THG-AG1.5 T can grow in TSB at pH 6.0–8.5 (optimum 6.5–7.5) and in the presence of additional 0–1.5 % (w/v) NaCl. Cells are able to hydrolyse Tween 80 and CMC but not L- tyrosine, casein, chitin, starch and DNA. Positive for alkaline phosphatase, esterase (C4), esterase lipase (C8), leucine arylamidase, valine arylamidase, cystine arylamidase, trypsin, a- chymotrypsin, acid phosphatase, naphthol-AS-BI-phosphohydrolase, b - galactosidase, a- glucosidase and b- glucosidase; weakly positive for lipase (C14); negative for a- galactosidase, b- glucuronidase, N -acetyl-b- glucosaminidase, a- mannosidase and a- fucosidase. Positive for reduction of nitrate to nitrites, assimilation of PNPG, arginine dihydrolase, D- glucose, L- arabinose, D- mannose, D- mannitol, maltose, and hydrolysis of urea, aesculin and gelatin; weakly positive for trisodium citrate; negative for indole production, glucose acidification, assimilation of N- acetyl-glucosamine, gluconate, caprate, adipate, malate and phenyl-acetate. Positive for utilization of salicin, melibiose, D- sorbitol, L- histidine, 2-ketogluconate, 3- hydroxy-butyrate, L- proline, sucrose, L- alanine and glycogen; weakly positive for valerate, D- ribose, itaconate, sodium malonate, sodium acetate and D,L- lactate; negative for L- fucose, propionate, 4-hydroxy-benzoate, L- rhamnose, inositol, suberate, 5-ketogluconate, 3-hydroxy-benzoate and L- serine. Weakly positive for assimilation of glycerol, D- fructose and trehalose; negative for erythritol, D- arabinose, D- xylose, L- xylose, D- adonitol, methyl b- D- xylopyranoside, D- galactose, L- sorbose, dulcitol, methyl a- D- mannopyranoside, methyl a- D- glucopyranoside, N -acetyl-D- glucosamine, amygdalin, arbutin, cellobiose, lactose, inulin, melezitose, raffinose, xylitol, gentiobiose, D- turanose, D- lyxose, D- tagatose, D- fucose, D- arabitol and L- arabitol. The predominant respiratory quinone is menaquinone-8 (MK-8); and iso-C 15: 0 , C 15: 1 Ɯ 6 c , C 16: 0 , iso-C 17: 0 , C 17: 0 , C 18: 0 and summed feature 3 (C 16: 1 Ɯ 7 c and/or C 16: 1 Ɯ 6 c ) are the major components of the cellular fatty acids (Ȅ7 %). The major polar lipids are a phosphoglycolipid, six unidentified glycolipids and an unidentified aminophospholipid. The polyamine is spermidine. The novel strain exhibits tolerance to UV irradiation (>1500 J m ‒2 ) and to gamma radiation (>12 kGy). The G+C content of genomic DNA of strain THG-AG1.5 T is 74.8 mol%. The peptidoglycan amino acids are alanine, valine, glutamic acid, glycine, ornithine, lysine and aspartic acid. The whole-cell-wall sugars are ribose, mannose and glucose. Strain THG-AG1.5 T (=KACC 18850 T =CCTCC AB 2016078 T ) was isolated from a rhizosphere soil sample of Hibiscus syriacus L. (Mugunghwa flower) , collected in Kyung Hee University , Yongin , Gyeonggi , Republic of Korea .