Rhodocybe tugrulii (Agaricales, Entolomataceae), a new species from Turkey and Estonia based on morphological and molecular data, and a new combination in Clitocella (Entolomataceae) Author Vizzini, Alfredo Author Baroni, Timothy J. Author Sesli, Ertugrul Author Antonín, Vladimír Author Saar, Irja text Phytotaxa 2016 2016-06-30 267 1 1 15 http://dx.doi.org/10.11646/phytotaxa.267.1.1 journal article 10.11646/phytotaxa.267.1.1 1179-3163 13658370 Rhodocybe tugrulii Vizzini, E. Sesli, T. J. Baroni, Antonín & I. Saar , sp. nov. (Figs. 3‒6) MycoBank:—MB 814883 Diagnosis:—Distinguished by grey to beige or slightly reddish brown basidiomata, small, convex to hemispheric pileus, decurrent lamellae, pruinose and cylindrical stipe, subcylindrical or clavate, thin-walled, 2‒4-spored, clampless basidia and hyaline, somewhat angular, broadly ellipsoid, sometimes obscurely bumpy, minutely angled, inamyloid, 5.9 × 4.9 μm basidiospores. Holotype :— TURKEY . Trabzon Province , Macka District , Zigana Mountain , among moss, around the base of Picea orientalis , 40°39´58´´ N and 39°25´05´´ E , 1714 m alt., 16 September 2014 , E . Sesli, KATO Fungi 3340 ( KATO !). Etymology:—the species name was given in honour of Tugrul Sesli, son of Ertugrul and the collector of the fungus. Description:— Basidiomata clitocyboid. Pileus 10‒40 mm , convex to hemispherical, not regular, broadly depressed, gray (Pale Drab-Gray, Smoke Gray, Plate XLVI) to beige (Yellow Ocher, Ochraceous-Tawny, Plate XV; Avellaneous, Plate XL), slightly reddish brown (Etruscan Red, Plate XXVII) when old or injured; turning slightly reddish with KOH and blackening with ammonia solution ; surface cracked, slightly pitted or ribbed especially when mature, dry, minutely felty-tomentose, at first entirely covered by a white pruina, margin irregular and incurved especially when young. Lamellae typically decurrent and subdistant, concolorous with the pileus surface or somewhat lighter (Pale Smoke Gray, Plate XLVI); moderately thick, fragile, margin regular, L = 30‒35, l = 2‒5. Stipe 10‒25 × 2‒5 mm , cylindrical, generally curved, pruinose, fibrous, concolorous with the pileus, becoming darker (Neutral Grey, Plate LIII) when handled, sometimes tapering toward the base, but the base typically thickened, covered with white mycelium and debris. The mycelium sometimes covers the other parts of the stipe. Context thin, up to 5 mm thick at pileus centre, white to grey-beige. Odour strongly mealy. Taste indistinct. FIGURE 3. R. tugrulii : a–d. basidiomes (b–d in situ. a–c = Sesli 3340 holotype, d = TU101325 paratype). Scale bars: a–b= 10 mm, c= 20 mm. Photos: a–c by E. Sesli, d by Vello Liiv (UNITE: https://unite.ut.ee/bl_forw.php?nimi=UDB011605). FIGURE 4. R. tugrulii : a–b. basidia and basidioles (in ammoniacal Congo Red, from Sesli 3340 holotype). Scale bars: a= 20 μm, b= 10 μm. Photos by E. Sesli. FIGURE 5. R. tugrulii : a–d. basidiospores (a and c= SEM, b and d= LM, b. in Melzer’s reagent, d. in 3% KOH, all from Sesli 3340 holotype). Scale bars: a and c= 1 μm, b and d= 5 μm. Photos: a, c and d by T.J. Baroni, b by E. Sesli. Basidiospores [95/3/2] (4.8) 5‒6.9 (7.2) × (4.0) 4.3‒5.5 (5.9) μm, on average 5. 9 × 4.9 μm, Q = 1.1‒1.5, Qm = 1.2, hyaline, with numerous guttules, subglobose to broadly ellipsoid and somewhat angular in profile view, sometimes obscurely bumpy, minutely angular in polar view (9‒12 facets), with a long apiculus, thin-walled and inamyloid. Basidia 25‒35 × 6‒8 (9) μm, 2- or 4-spored (mostly 4-spored), clavate or subcylindrical, without basal clamp, thin-walled. Basidioles similar to basidia, 20‒25 × 4‒6 μm, narrowly clavate, subfusoid or subcylindrical. Cheilo-, pleuro- and pseudocystidia absent. Hyphae of hymenophoral trama regular, hyphal system monomitic, cylindrical, thin-walled, 35‒200 × 3‒11 μm, composed of cylindrical (4.8‒7.2 um in diam.) repent hyphae, with yellow-brown shiny granular or spirally encrusting pigments. Pileipellis two-layered, a thin hyaline repent layer, 2‒4 cells deep, of narrow cylindrical hyphae, 2.8–4 μm in diam., overlying a multi-celled layer of interwoven hyphae with brown or yellow-brown, granular and spirally pigment encrusting hyphae, 4‒8 μm in diam. Pileus context composed of interwoven, hyaline, cylindrical or slightly enlarged hyphae, 4‒10 μm in diam. Stipitipellis a cutis of cylindrical, parallel, thin-walled, smooth, 5.5‒15 μm wide hyphae. Caulocystidia absent. FIGURE 6 . R. tugrulii : a. hyphae of lamellae, b. elements of pileipellis (in ammoniacal Congo Red, from Sesli 3340 holotype). Scale bars: a–b= 20 μm. Photos by E. Sesli. Habit, habitat and distribution: — Solitary or gregarious in coniferous litter ( Picea , Pinus ). Autumn, known only from Turkey and Estonia so far. Paratype : — ESTONIA . Lääne County , Nõva Community, Nõva Landscape Reserve, Lepaauk, mixed pine forest ( Pinus sylvestris , Picea abies , Betula sp. , Juniperus communis ) on dunes, 59°13´44´´N , 23°42´42´´E , 7 September 2007 , I. Saar, TU101325 (TU!). Additional material examined: — AUSTRALIA . Tasmania , Mount Field National Park, Marriotts Falls, 42°43´58´´S , 146°39´25´´E , 2 June 2001 , G. Gates E1213 & D. Ratkowsky ( paratype of Rhodocybe pallidogrisea CORT-013944 , (CORT!).