Morphological and molecular identification for two new species of woodinhabiting macrofungi (Basidiomycota) from Yunnan-Guizhou Plateau, China Author Yang, Yang 0000-0001-8054-948X College of Biodiversity Conservation, Southwest Forestry University, Kunming 650224, P. R. China & fungiyoung @ 163. com; https: // orcid. org / 0000 - 0001 - 8054 - 948 X Author Li, Rong 0000-0003-1524-8247 Yunnan Key Laboratory for Fungal Diversity and Green Development, Kunming Institute of Botany, Chinese Academy of Science, Kunming 650201, P. R. China & pearscus 0728 @ 163. com; https: // orcid. org / 0000 - 0003 - 1524 - 8247 Author Liu, Chaomao 0000-0003-2673-8890 College of Biodiversity Conservation, Southwest Forestry University, Kunming 650224, P. R. China & lcm 1987 swkx @ 163. com; https: // orcid. org / 0000 - 0003 - 2673 - 8890 Author Zhao, Changlin 0000-0002-8668-1075 College of Biodiversity Conservation, Southwest Forestry University, Kunming 650224, P. R. China & Yunnan Key Laboratory for Fungal Diversity and Green Development, Kunming Institute of Botany, Chinese Academy of Science, Kunming 650201, P. R. China & fungichanglinz @ 163. com; https: // orcid. org / 0000 - 0002 - 8668 - 1075 text Phytotaxa 2023 2023-03-30 591 1 1 18 http://dx.doi.org/10.1094/PDIS-04-22-0755-PDN journal article 10.11646/phytotaxa.591.1.1 1179-3163 7784136 Resupinatus yunnanensis Y. Yang & C.L. Zhao , sp. nov. Figs. 1 , 3 , 6 , 7 MycoBank no.: MB 847020 Holotype : — CHINA . Yunnan Province , Pu’er , Jingdong County , Taizhong Township , Xujiaba , Ailaoshan Ecological Station , E 100°53′41′′ , N 24°23′36′′ , elev. 1800 m , on the fallen branch of angiosperm, 24 August 2018 , CLZhao 8651 ( SWFC !), GenBank No. (ITS OP901839; nLSU OP904197). Etymology : — yunnanensis (Lat.) : refers to the locality ( Yunnan Province ) of the type specimen. Basidiomata : —Annual, resupinate to cupulate, soft gelatinous when fresh, fragile when drying, up to 12 cm long, 3 cm wide, 0.5 mm thick, cups globose or depressed-globose, up to 150–500 μm in diameter, aggregated in groups, 3–6 per mm, buff (4A4) to slightly brown (5B/C7), covered in a dense mat of white hairs; hymenophore cyphelloid, buff (4A4) to slightly brown (5B/C7) when fresh, greyish (19C2) to pale mouse-grey (7C2) upon drying; sterile margin indistinct and slightly brown; subiculum felty, loosely attached to the substrate and densely packed, up to 200 μm thick. Hyphal structure : — Hyphal system monomitic; generative hyphae with clamp connections, colorless, thin-walled, branched, interwoven, 1.5–3.5 μm in diam; IKI–, CB–, tissues unchanged in KOH. presence of crystal encrusted branched hyphae with short finger-like projections at the tips. Hymenium : — Cystidia and cystidioles absent; basidia clavate, 12–36.5 × 4–8 µm, with 4-sterigmata and with a basal clamp connection, basidioles dominant, in shape similar to basidia, but slightly smaller. Basidiospores : —Ellipsoid, colorless, thin-walled, smooth, with oil drops, IKI–, CB–, 4.5–9(–9.5) × 3.5–7 µm, L = 6.68 µm, W = 4.72 µm, Q = 1.32–1.71 (n = 90/3), Qm = 1.42. FIGURE 5. Microscopic structures of Coniophora yunnanensis (drawn from the holotype). A. Basidiospores. B. Basidia and basidioles. C. A section of hymenium. Bars: A–C = 10 µm. Drawings by: Yang Yang FIGURE 6. Basidiomata of Resupinatus yunnanensis . Bars: A = 2 cm, B = 1 mm (holotype). Photos by: Yang Yang Additional specimens examined :— CHINA . Yunnan Province , Pu’er, Jingdong County , Taizhong Town , Ailaoshan , E 100°51′30′′ , N 24°25′42′′ , elev. 1800 m , on the fallen branch of angiosperm, 4 October 2017 , CLZhao 3802 ( SWFC !), GenBank No. (ITS OP901840 ); Chuxiong , Zixishan Forestry Park , E 101°24′06′′ , N 25°03′60′′ , elev. 2100 m , on the trunk of angiosperm, 1 July 2018 , CLZhao 7168 ( SWFC !), GenBank No. (ITS OP901838 ). Notes: Resupinatus alboniger (Pat.) Singer, Beih. (1977: 17) , R. cinerascens (Cleland) Grgur.(1997: 47) , R. hyalinus (Singer) Thorn, Moncalvo & Redhead (2006: 1148) and R. vinosolividus (Segedin) J.A. Cooper (2012: 1) resemble R. yunnanensis by having the elliptical basidiospores. However, Resupinatus alboniger is distinguished from R. yunnanensis by the brown hymenophore and irregular finger-like cystidia (23–35 × 8–10 µm) ( Gonou-Zagou et al. 2011 ); R. cinerascens differs in R. yunnanensis by having the grayish black hymenophore and diverticulate branching cystidia (19–35.5 × 2.5–6 µm) ( Mcdonald 2015 ); R. hyalinus is separated from R. yunnanensis by having the surface hairs and finger-like cystidia ( Mcdonald 2015 ); R. vinosolividus differs from R. yunnanensis by having the cheilocystidia at edge of basidiomata on top surface of gills ( Mcdonald 2015 ). Resupinatus yunnanensis resembles R. applicatus (Batsch) Gray (1821: 617) , R. huia (G. Cunn.) Thorn, Moncalvo & Redhead (2006: 1148) , R. incanus (Kalchbr.) Thorn, Moncalvo & Redhead (2006: 1148) and R. poriaeformis (Pers.) Thorn, Moncalvo & Redhead (2006: 1148) in having cupulate basidiomata. However, Resupinatus applicatus is different from R. yunnanensis by having the brown to black hymenophore with a lateral pseudostipe, diverticulate with finger-like cystidia and globose to subglobose basidiospores ( Mcdonald 2015 ); R. huia can be delimited from R. yunnanensis by its simple septa generative hyphae and oblong basidiospores ( Thorn et al. 2005 ); R. incanus differs from R. yunnanensis by grey to black basidiomata and oblong basidiospores ( Thorn et al. 2005 ); R. poriaeformis is distinguished from R. yunnanensis by dark brown or grey basidiomata, diverticulate cystidia, and globose to subglobose basidiospores and tan to ( Thorn et al. 2005 ).