Two new species of Caligonellidae (Acari: Raphignathoidea) from Crimea Author Khaustov, Alexander text Acarologia 2021 2021-10-26 61 4 910 927 https://www1.montpellier.inrae.fr/CBGP/acarologia/article.php?id=4474 journal article 10.24349/DZRV-GIdY 2107-7207 7159815 Caligonella quinqueocellata n. sp. Zoobank: 3FE8CEF5-57DF-4523-BF9A-9F15AE42AA02 ( Figures 1–8 ) Diagnosis — Female . Dorsal shield present, seta ve located on soft cuticle; prodorsum with five pairs of ocelli; one pair of pseudanal setae; ventral plate absent. Description Female ( Figs 1-5 ) — Length of idiosoma 460 (405), width 275 (255). Idiosomal dorsum ( Figs 1A , 3A , 5B, C ) ‒ Ovate. Dorsal idiosomal stria thick, usually double; dorsal shield well developed, ovate, with few sparsely distributed puncta and fine stria ( Fig. 5C ) and located in space between setae vi , ve , and c1 , its length 135 (130), width 68 (58). Five pairs of ocelli located posterolaterad setae sci , anterior ocellus much bigger than four posterior ( Figs 3A , 5B ). Cupuli ia , im and ip very large, almost round; ia located just posteriad ocelli, im posterolaterad c1 and ip laterad f . All dorsal idiosomal setae subequal, short, smooth and indistinctly blunt-tipped. Anal valves located dorsoterminal, weakly striated and with few sparsely distributed puncta; one pair of pseudanal setae. Lengths of dorsal setae: vi 17 (17), ve 17 (17), sci 20 (19), sce 21 (18), c1 16 (15), c2 18 (15), d 18 (15), e 17 (14), f 15 (13), h1 15 (12), h2 16, ps 15 (12). Idiosomal venter ( Figs 1B , 5D ) ‒ Ventral idiosoma without plate between coxae, striated. Coxal fields I-II and III-IV with sparsely distributed puncta; coxal field IV with posterior plate-like projection delineated by stria and sparsely distributed puncta. All ventral setae smooth and pointed. Three pairs of aggenital and one pair of genital setae ( Fig. 5D ); cupuli ih located anterolaterad ag3 . Lengths of ventral setae: 1a 27 (26), 1b 28 (26), 1c 17 (17), 2c 15 (13), 3a 25 (26), 3b 17 (13), 4a 20 (19), 4c 20 (16), ag1 18 (18), ag2 16 (12), ag3 14 (12), g 12 (11). Gnathosoma ( Figs 2 , 3B, C , 5A ) ‒ Stylophore almost oval ( Fig. 2 A ), distinctly punctate in anterior 2/3 and finely striated in posterior 1/3 parts, its length 110 (100), width 91 (75); peritremes distinctly curved in central part of stylophore ( Figs 2A , 3B , 5A ); cheliceral levers large, oval, almost as long as cheliceral stylets ( Fig. 5C ). Palpal supracoxal setae ep () located in deep depression with terminal pore; palpal chaetotaxy: Tr 0, Fe 1 d (), Ge 1 ( d ), Ti 3 ( d , l’ , l” ), Ta 7(1) ( ba , bp , va , acmϛ , ul’ϛ , ul”ϛ , sulϛ , ω ); tibial claw well developed, slightly hooked; all palpal setae smooth, eupathidia acmϛ , ul’ϛ , ul”ϛ , sulϛ with slightly swollen tips, other palpal setae pointed. Subcapitulum with few sparsely distributed puncta ( Fig. 2B ); all subcapitular setae smooth, setae m pointed, adoral setae weakly blunt-tipped; lengths of subcapitular setae: m 33, or1 6, or2 8, length of palptarsal solenidion ω 4 (3). Figure 1 Caligonella quinqueocellata n. sp. , female: A – idiosomal dorsum, B – idiosomal venter. Legs ( Fig. 4 ) ‒ Lengths of legs (excluding claws): I 300 (280), II 230 (210), III 250 (220), IV 295 (250). Leg I ( Fig. 4A ) longer than other legs. Leg setation: Tr 1 v ′ (), Fe 2 ( d , bv” ), Ge 6 ( d , l’ , l” , v ′ , v” , k ), Ti 5(2) ( d , l’ , l” , v ′ , v” , φ , φp ), Ta 16(1) ( ft ′ , ft” , tc ′ ϛ , tc”ϛ , p ′ ϛ , p”ϛ , it ′ , a ′ , a” , u ′ , u” , vs , pv ′ , pv” , pl’ , pl” , ω ). Supracoxal seta of leg I ( el ) located in deep depression with terminal pore; seta k of genu located in deep depression; all leg setae smooth; setae tc )(and ( p ) eupathid-like, blunt-tipped, other leg setae pointed; solenidion ω 8 (7) digitiform, solenidia φ 4 (4) and φp 12 (12) baculiform. Leg II ( Fig. 4B ). Leg setation: Tr 1 v ( ′ ), Fe 2 ( d , bv” ), Ge 5 ( d , Figure 2 Caligonella quinqueocellata n. sp. , female: A – gnathosoma, dorsal aspect, B – palptarsus, dorsal aspect. C – subcapitulum. l’ , l” , v ′ , v” ), Ti 5 ( d , l’ , l” , v ′ , v” ), Ta 11(1) ( ft ′ , tc ′ , tc” , p”ϛ , it” , a ′ , a” , u ′ , u” , pv ′ , pv” , ω ). All leg setae smooth; seta p”ϛ eupathid-like, blunt-tipped; other leg setae pointed; solenidion ω 7 (7) digitiform. Leg III ( Fig. 4C ). Leg setation: Tr 1 v ′ (), Fe 2 ( d , ev ′ ), Ge 2 ( d , v ′ ), Ti 4 ( d , l” , v ′ , v” ), Ta 9 ( tc ′ , tc” , it ′ , a ′ , a” , u ′ , u” , pv ′ , pv” ). All leg setae smooth and pointed. Leg IV ( Fig. 4D ). Leg setation: Tr 1 ( v ′ ), Fe 2 ( d , ev ′ ), Ge 2 ( d , v ′ ), Ti 4 ( d , l” , v ′ , v” ), Ta 9 ( tc ′ , tc” , it ′ , a ′ , a” , u ′ , u” , pv ′ , pv” ). All leg setae smooth and pointed. Larva ( Figs 6-8 ) — Length of idiosoma 245, width 170. Idiosomal dorsum ( Fig. 6A ) ‒ completely striated, without dorsal shield. Dorsal setae and cupuli as in female. With one pair of large anterior ocelli, posterior ocelli indistinct. Anal opening terminal. Lengths of dorsal setae: vi 11, ve 10, sci 11, sce 12, c1 10, c2 10, d 9, e 10, f 11, h1 13, h2 11, ps 7. Idiosomal venter ( Fig. 6B ) ‒ Ventral idiosoma completely striated. Coxal field I with two setae ( 1a , 1b ), coxal field III with one setae ( 3a ); genital and aggenital setae absent. All ventral setae smooth and pointed; cupuli ih located anteromesad h2 . Lengths of ventral setae: 1a 19, 1b 15, 3a 16. Figure 3 Caligonella quinqueocellata n. sp. , female: A – anterolateral part of prodorsum, B – anterior part of stylophore, dorsal aspect. C – anterior part of stylophore, ventral aspect. Gnathosoma ( Fig. 7 ) ‒ in general similar to that of female except following: peritremes straight, subcapitulum without setae m , palptarsus with setae acm and sul simple (not eupathidia). Length of stylophore 56, width 53, lengths of setae: or1 6, or2 7, length of palptarsal solenidion ω 3. Legs ( Fig. 8 ) ‒ Lengths of legs: I 170, II 130, III 145. Leg I ( Fig. 8A ) longer than other legs. Leg setation: Tr 0, Fe 2 d (, bv” ), Ge 5 ( l’ , l” , v ′ , v” , k ), Ti 5(2) ( d , l’ , l” , v ′ , v” , φ , φp ), Ta 15(1) ( ft ′ , ft” , tc ′ , tc” , p ′ ϛ , p”ϛ , a ′ , a” , u ′ , u” , vs , pv ′ , pv” , pl’ , pl” , ω ). Seta k of genu located in shallow depression; all leg setae smooth; setae p () eupathid-like, blunt-tipped, other leg setae pointed; solenidion ω 7 digitiform, solenidia φ 3 and φp 7 baculiform. Leg II ( Fig. 8B ). Leg setation: Tr 0, Fe 2 ( d , bv” ), Ge 4 ( l’ , l” , v ′ , v” ), Ti 5 ( d , l’ , l” , v ′ , v” ), Ta 10(1) ( ft ′ , tc ′ , tc” , p”ϛ , a ′ , a” , u ′ , u” , pv ′ , pv” , ω ). All leg setae smooth; seta p”ϛ eupathid-like, blunt-tipped; other leg setae pointed; solenidion ω 6 digitiform. Leg III ( Fig. 8C ). Leg setation: Tr 0, Fe 2 d ,( ev ′ ), Ge 1 ( v ′ ), Ti 4 ( d , l” , v ′ , v” ), Ta 8 ( tc ′ , tc” , a ′ , a” , u ′ , u” , pv ′ , pv” ). All leg setae smooth and pointed. Type material — Female holotype , slide ZISP T-Cal-01, Crimea , Arabat Spit, 45°17 ′ 54.0 ″ N 35°28 ′ 19.0 ″ E , 14 June 2021 , moss on sandy soil, coll. A.A. Khaustov. Paratypes : 1 female , 1 larva , same data. Type deposition — The holotype of the new species is deposited in the acarological collection of the Zoological Institute of RAS, St. Petersburg , Russia ; paratypes are deposited in the mite collection of the Tyumen State University Museum of Zoology, Tyumen , Russia . Figure 4 Caligonella quinqueocellata n. sp. , female: A-D – legs I-IV respectively, dorsal aspect. Figure 5 DIC micrographs of Caligonella quinqueocellata n. sp. , female: A – stylophore, dorsal aspect, B – anterolateral part of prodorsum. C – dorsal shield, D – genital area. Etymology — The name of the new species is combined from two Latin words quinque meaning five and ocellus and refers to presence of five pairs of ocelli in female. Differential diagnosis — The new species differs from all species of Caligonella in having five pairs of ocelli on prodorsum (only two in other species), The new species is most similar to Caligonella scutovata Smith-Meyer and Ueckermann described from South Africa (SmithMeyer and Ueckermann 1989), by the presence of dorsal idiosomal shield and in having one pair of pseudanal setae. The new species differs from C . scutovata by the absence of ventral plate between coxae (present in C. scutovata ) and location of setae ve on soft cuticle ( ve located on dorsal shield in C. scutovata ).