New section and species in Talaromyces
Author
Sun, Bing-Da
China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China & State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
https://orcid.org/0000-0003-0272-6422
Author
Chen, Amanda J.
China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
https://orcid.org/0000-0001-5294-9574
Author
Houbraken, Jos
Westerdijk Fungal Biodiversity Institute, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
Author
Frisvad, Jens C.
Department of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, Denmark
Author
Wu, Wen-Ping
Novozymes China, No. 14, Xinxi Rd, Shangdi, Beijing, China
Author
Wei, Hai-Lei
Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China
https://orcid.org/0000-0001-6554-009X
Author
Zhou, Yu-Guang
China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
Author
Jiang, Xian-Zhi
Microbiome Research Center, Moon (Guangzhou) Biotech Ltd., Guangzhou 510535, China
jxz@moonbio.com
Author
Samson, Robert A.
Westerdijk Fungal Biodiversity Institute, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
r.samson@wi.knaw.nl
text
MycoKeys
2020
68
75
113
http://dx.doi.org/10.3897/mycokeys.68.52092
journal article
http://dx.doi.org/10.3897/mycokeys.68.52092
1314-4049-68-75
BBED0AD34D38552BA75860CC3EDB4AEE
Talaromyces guizhouensis B.D. Sun, A.J. Chen, Houbraken & Samson
sp. nov.
Fig. 9
Typus.
China
, Guizhou, soil, 2014, isolated by X.Z. Jiang, Holotype CBS H-22835, culture ex-holotype CBS 141837= DTO 340-G8.
Additional material examined.
Malaysia
, Langkawi, soil from rainforest, 2007, isolated by J. Houbraken, culture DTO 054-C8. Malaysia, Langkawi, soil from rainforest, 2007, isolated by J. Houbraken, culture DTO 054-A7.
ITS barcode.
MN864277. Alternative identification markers:
BenA
= MN863346,
CaM
= MN863323,
RPB2
= MN863335.
Diagnosis.
Talaromyces guizhouensis
grows poorly on CREA and DG18, does not produce synnemata as well as ascospores.
In.
Talaromyces section Subinflati
Colony diam, 7 d (mm).
CYA 8-9; CYA 30 °C 10; CYA 37 °C No growth; MEA 24-27; MEA 30 °C 18-19; OA 27-29; YES 12-13; CREA 2-3; CYAS No growth; DG18 4-5.
Colony characters.
CYA 25 °C, 7 d: Colonies moderately deep, plane; margins entire; mycelium white; texture floccose; sporulation absent; soluble pigments absent; exudates clear droplets; reverse saffron (10). MEA 25 °C, 7 d: Colonies moderately deep, raised at center, plane; margins entire; mycelium white; texture floccose; sporulation moderately dense; conidia
en masse
pistachio green (92); soluble pigments absent; exudates absent; reverse saffron (10). YES 25 °C, 7 d: Colonies moderately deep, raised at center, plane; margins entire; mycelium white; texture floccose; sporulation absent; soluble pigments absent; exudates clear droplets; reverse cream white. DG18 25 °C, 7 d: Colonies moderately deep, plane; margins entire; mycelium white; texture floccose; sporulation absent; soluble pigments absent; exudates absent; reverse cream white. OA 25 °C, 7 d: Colonies moderately deep, raised at center, plane; margins entire; mycelium white; texture floccose; sporulation moderately dense; conidia
en masse
pistachio green (92); soluble pigments absent; exudates clear droplets; reverse greyish lavender (98) at center, fading into saffron (10). CREA 25 °C, 7 d: Poor growth, acid production absent.
Micromorphology.
Conidiophores biverticillate, stipes smooth to finely rough, 150-300
x
3-4.5
μm
, metulae 3-5, divergent, 11-13
x
3-5
μm
; phialides 3-5, acerose to flask shaped, 9-10
x
3-3.5
μm
; conidia finely rough, subglobose to fusiform, 2.5-4.5
x
2.5-3
μm
. Ascomata not observed.
Notes.
section
Subinflati
previously contained two species namely
T. subinflatus
and
T. palmae
. These species do not resemble each other, although both grow poorly on CREA and DG18 (
Yilmaz et al. 2014
).
Talaromyces tzapotlensis
was included more recently (
Peterson and
Jurjevic
2017
) and we here expand this section with
T. guizhouensis
and
T. resedanus
. Like the other species in this section,
T. guizhouensis
also grows poorly on CREA and DG18. This species is phylogenetically related to
T. subinflatus
, but the latter grows very restrictedly on common media except MEA (
Yilmaz et al. 2014
).
Talaromyces palmae
produces indeterminate synnemata and short stipes (up to 85
μm
) (
Yilmaz et al. 2014
) and these are not observed in
T. guizhouensis
. Furthermore,
T. tzapotlensis
grows faster on most media (e.g., 29-30
vs
8-9 mm on CYA; 10-11
vs
4-5 mm on DG18; 20-22
vs
2-3 mm on CREA, all diam. after 7 days (
Peterson and
Jurjevic
2017
) and
T. resedanus
does not grow on CREA and produces smaller conidia measuring 2-3
x
1.5-2
μm
.
Etymology.
Latin,
guizhouensis
, refers to its origin, isolated from Guizhou, China.
Figure 9.
Talaromyces guizhouensis
CBS 141837T
a
colonies from left to right (top row) CYA, MEA, YES and OA; (bottom row) CYA reverse, MEA reverse, DG 18 and CREA
b-g
conidiophores and conidia. Scale bars: 10
μm
(
b-g
).