Kodamaea hongheensis f. a., sp. nov., Kodamaea ovata f. a., sp. nov. and Kodamaea yamadae f. a., sp. nov., three new yeast species of Kodamaea (Saccharomycetales, Debaryomycetacae) from China Author Chai, Chun-Yue School of Life Science and Agricultural Engineering, Nanyang Normal University, Nanyang 473061, China & Research Center of Henan Provincial Agricultural Biomass Resource Engineering and Technology, Nanyang 473061, China Author Gao, Wan-Li School of Life Science and Agricultural Engineering, Nanyang Normal University, Nanyang 473061, China Author Li, Ying School of Life Science and Agricultural Engineering, Nanyang Normal University, Nanyang 473061, China Author Yan, Zhen-Li State Key Laboratory of Motor Vehicle Biofuel Technology, Henan Tianguan Enterprise Group Co., Ltd., Nanyang 473000, China Author Hui, Feng-Li School of Life Science and Agricultural Engineering, Nanyang Normal University, Nanyang 473061, China & Research Center of Henan Provincial Agricultural Biomass Resource Engineering and Technology, Nanyang 473061, China fenglihui@yeah.net text MycoKeys 2022 2022-04-29 89 121 137 http://dx.doi.org/10.3897/mycokeys.89.81119 journal article http://dx.doi.org/10.3897/mycokeys.89.81119 1314-4049-89-121 414359D6F57D580695D9A951816962D9 Kodamaea hongheensis C.Y. Chai & F.L. Hui sp. nov. Fig. 2 Holotype. China, Yunnan Province, Honghe Prefecture, Luxi County, in rotting wood in Jiuxi Mountain Forest Park, April 2017, K.F. Liu & Z.W. Xi (holotype NYNU 17423T, ex-holotype CICC 33265). Etymology. The specific epithet Kodamaea hongheensis refers to the geographic origin of the type strain, Honghe Prefecture, Yunnan. Description. In YM broth, after 3 days at 25 °C, cells are ovoid to elongate (3-5 x 3-7 μm ) and occur singly or in pairs (Fig. 2a ). Sediment is formed after a month, but a pellicle is not observed. On YM agar, after 3 days at 25 °C, colonies are white to cream-colored, butyrous and smooth with entire margins. In Dalmau plate culture on CM agar, pseudohyphae are formed but not true mycelia (Fig. 2b ). Asci or signs of conjugation are not seen on sporulation media. Glucose, maltose and trehalose are fermented but not galactose, sucrose, melibiose, lactose, cellobiose, melezitose, raffinose, inulin, or xylose. Glucose, glucosamine, d-xylose, sucrose, maltose, trehalose, methyl α-d-glucoside , cellobiose, salicin, melezitose, glycerol, ribitol, d-glucitol, d-mannitol, d-glucono-1, 5-lactone, 2-keto-d-gluconate, succinate, citrate and ethanol are assimilated as sole carbon sources. Galactose, l-sorbose, d-ribose, d-arabinose, l-arabinose, l-rhamnose, melibiose, lactose, raffinose, inulin, erythritol, xylitol, galactitol, myo -inositol, d-gluconate, d-glucuronate, dl-lactate and methanol are not assimilated. l-lysine, glucosamine and d-tryptophan are assimilated as sole nitrogen sources. Nitrate, nitrite, ethylamine, cadaverine, creatine, creatinine and imidazole are not assimilated. Growth is observed at 35 °C but not at 37 °C. Growth in the presence of 0.01% cycloheximide is positive, but growth in the presence of 10% NaCl plus 5% glucose and 1% acetic acid is negative. Starch-like compounds are not produced. Urease activity and diazonium blue B reactions are negative. Figure 2. Morphology of Kodamaea hongheensis (NYNU 17423, holotype) a budding cells in YM broth after 3 d b simple pseudohyphae on CM agar after 14 d. Scale bars: 10 μm . Additional isolate examined. China, Yunnan Province, Honghe Prefecture, Luxi County, in rotting wood in Jiuxi Mountain Forest Park, April 2017, K.F. Liu & Z.W. Xi (NYNU 17409). Notes. Two strains NYNU 17409 and NYNU 17423, both representing K. hongheensis , were grouped in an independent lineage and are related to K. laetipori . K. hongheensis differed from its closest relative K. laetipori by 2.9% substitutions in the LSU rDNA sequence. However, the ITS sequence of K. hongheensis could not be successfully aligned with the type strain of K. laetipori because its ITS sequences are not currently available from either the NCBI GenBank database or the CBS database. Physiologically, K. hongheensis can be differentiated from K. laetipori ( Lachance and Kurtzman 2011 ) based on growth in l-sorbose, d-arabinose, d-gluconate, and dl-lactate, which are positive for K. laetipori and negative for the new species. Moreover, K. laetipori grows in the presence of 0.1% cycloheximide and 10% NaCl plus 5% glucose, but K. hongheensis does not.