Three novel species of Distoseptispora (Distoseptisporaceae) isolated from bamboo in Jiangxi Province, China Author Zhai, Zhi-Jun Bioengineering and Technological Research Centre for Edible and Medicinal Fungi, College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China & Jiangxi Key Laboratory for Conservation and Utilization of Fungal Resources, College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China Author Yan, Jun-Qing https://orcid.org/0000-0003-1128-5171 Bioengineering and Technological Research Centre for Edible and Medicinal Fungi, College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China & Jiangxi Key Laboratory for Conservation and Utilization of Fungal Resources, College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China Author Li, Wei-Wu Bioengineering and Technological Research Centre for Edible and Medicinal Fungi, College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China Author Gao, Yang Bioengineering and Technological Research Centre for Edible and Medicinal Fungi, College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China & Jiangxi Key Laboratory for Conservation and Utilization of Fungal Resources, College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China Author Hu, Hai-Jing Bioengineering and Technological Research Centre for Edible and Medicinal Fungi, College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China & Jiangxi Key Laboratory for Conservation and Utilization of Fungal Resources, College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China Author Zhou, Jian-Ping Bioengineering and Technological Research Centre for Edible and Medicinal Fungi, College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China & Jiangxi Key Laboratory for Conservation and Utilization of Fungal Resources, College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China Author Song, Hai-Yan Bioengineering and Technological Research Centre for Edible and Medicinal Fungi, College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China & Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education of the P. R. China, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China Author Hu, Dian-Ming https://orcid.org/0000-0002-4750-2871 Bioengineering and Technological Research Centre for Edible and Medicinal Fungi, College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China & Jiangxi Key Laboratory for Conservation and Utilization of Fungal Resources, College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China hudianming1@163.com text MycoKeys 2022 2022-03-22 88 35 54 http://dx.doi.org/10.3897/mycokeys.88.79346 journal article http://dx.doi.org/10.3897/mycokeys.88.79346 1314-4049-88-35 B5256730C65A504F8F61A15434CC47BB Distoseptispora yunjushanensis Z. J. Zhai & D. M. Hu sp. nov. Fig. 4 Etymology. The epithet refers to the collecting site from the Yunjushan Mountain in China. Holotype. HFJAU10005 Description. Saprobic on decaying bamboo culms submerged in freshwater habitats. Sexual morph : Undetermined. Asexual morph : Hyphomycetous. Colonies effuse, olivaceous or dark brown, hairy, velvety. Mycelium mostly immersed, consisting of branched, septate, smooth, subhyaline to pale brown hyphae. Conidiophores 100-175 μm x 5.5-10 μm (x- = 129 x 7.1 μm , n = 30), single or in groups of 2 or 3, macronematous, mononematous, erect, straight or slightly flexuous, 4-7-septate, unbranched, olivaceous to dark brown, smooth, cylindrical, rounded at the apex. Conidiogenous cells monoblastic, integrated, terminal, determinate, pale to dark brown, cylindrical. Conidia 39-67.5(-77) μm x (7-)9.5-13.5(-16.5) μm (x- = 52 x 12 μm , n = 30), acrogenous, solitary, obpyriform or obclavate, thick-walled, tapering towards the rounded apex, slightly curved, truncate at the base, 7-13-distoseptate, guttulate, smooth-walled, olivaceous, dark brown when mature, sometimes with the percurrent proliferation which forms another conidium from the conidial apex. Figure 4. Distoseptispora yunjushanensis (HFJAU 10005, holotype) a, b colonies on bamboo culms c-f conidiophores with conidia g-i young conidia j-l mature conidia m conidium with proliferation n germinating conidium o, p culture on PDA from above and reverse. Scale bars: 100 µm ( a, b ), 20 µm ( c-f, m, n ), 5 µm ( g-l ). Cultural characteristics. Conidia germinating on PDA within 24 h and germ tubes produced from both ends. Colonies on PDA reaching 12-18 mm diam. at 14 days at 25 °C, in natural light, with fluffy, dense, thin olivaceous mycelium in the centre, becoming sparse and paler at the entire margin; reverse dark brown, pale brown at the smooth margin. Material examined. China , Jiangxi Province , Jiujiang City , Yongxiu County , Yunjushan Mountain , alt. 672.5 m , 29.23°N , 115.59°E , on decaying bamboo culms submerged in a freshwater stream, 28 Apr 2020 , Z. J. Zhai and W. W. Li , YJS-42 (HFJAU 10005, holotype ), ex-type living culture, JAUCC 4723 = JAUCC 4724 . Notes. In the phylogenetic analysis, D. yunjushanensis clusters with D. obclavata and D. rayongensis with moderate support (BS/PP = 81/1.00). However, D. yunjushanensis is easily distinguished from D. obclavata by its comparatively wider (5.5-10 μm vs. 5-7 μm ) conidiophores and conidia ((7-)9.5-13.5(-16.5) μm vs. 9-11 μm ) ( Luo et al. 2019 ). Moreover, the percurrent proliferation of conidia was not observed in D. obclavata ( Luo et al. 2019 ). Distoseptispora yunjushanensis has shorter conidia (39-67.5(-77) μm vs. (36-)60-106(-120) μm ) and wider conidiophores (5.5-10 μm vs. 3.5-5.5 μm ) than those of D. rayongensis ( Hyde et al. 2020 ). The morphology of D. yunjushanensis is similar to D. guttulata and D. songkhlaensis in having the obclavate conidia, but differs in having wider (5.5-10 μm vs. 3.5-5.5 μm and 4-5.5 μm ) conidiophores, shorter (39-67.5(-77) μm vs. 75-130(-165) μm and 44-125 μm ) and proliferating conidia ( Yang et al. 2018 ; Dong et al. 2021 ). Additionally, D. yunjushanensis can be distinguished from D. guttulata by its distoseptate conidia ( Yang et al. 2018 ).