Erysipelothrix inopinata sp. nov., isolated in the course of sterile filtration of vegetable peptone broth, and description of Erysipelotrichaceae fam. nov. Author Susanne Verbarg Author Holger Rheims Author Sabine Emus Author Anja Frühling Author Reiner M. Kroppenstedt Author Erko Stackebrandt Author Peter Schumann text International Journal of Systematic and Evolutionary Microbiology 2004 54 221 225 journal article 10.1099/ijs.0.02898-0 Description of Erysipelotrichaceae fam. nov. Erysipelotrichaceae (E.ry.si.pe.lo.tri.cha'ce.ae. N.L. fem. n. Erysipelothrix type genus of the family; - aceae ending to denote a family; N.L. fem. pl. n. Erysipelotrichaceae the Erysipelothrix family). The description is based on the generic description of Erysipelothrix (Jones, 1986; data obtained in this study). Straight or slightly curved, slender rods; some strains have a tendency to form long filaments. Non-motile. Endospores are not produced. Menaquinones are absent. Murein belongs to the B-cross-linking type , having L-alanine in position 3 of the peptide subunit and an interpeptide Taxa: 1, E. inopinata MF-EP02T; 2, E. rhusiopathiae DSM 5055T; 3, E. rhusiopathiae DSM 5056; 4, E. rhusiopathiae DSM 5057; 5, E. rhusiopathiae DSM 5058; 6, E. tonsillarum DSM 14972T. According to API 32 STREPT, all strains were positive for glycyl tryptophan arylamidase, pyroglutamic acid arylamidase and acid production from glucose. All strains were negative for oxidase, aminopeptidase, hydrolysis of starch, gelatin, DNA and casein, urease, acid from mannitol, sorbitol, raffinose, sucrose, L-arabinose, D-arabitol, cyclodextrin, glycogen, pullulan, maltose, melibiose, melezitose and tagatose, β-glucuronidase, production of acetoin and hydrolysis of hippurate. As determined with Biolog GP, all strains use the following substrates: adenosine, uridine, methyl pyruvate, N -acetylglucosamine and α-D-glucose. All strains are negative for methyl β-D-glucoside, D-tagatose, lactamide, alaninamide, D-arabitol, lactulose, methyl α-D-mannoside, D-lactic acid methyl ester, D-alanine, β-cyclodextrin, maltose, palatinose, turanose, L-lactic acid, L-alanine, dextrin, maltotriose, xylitol, D-malic acid, L-asparagine, glycogen, D-mannitol, D-raffinose, L-malic acid, inulin, L-fucose, L-rhamnose, acetic acid, L-glutamic acid, adenosine 5'-monophosphate, mannan, D-melezitose, α-hydroxybutyric acid, monomethyl succinate, glycyl-L-glutamic acid, thymidine 5'-monophosphate, Tween 40, D-galacturonic acid, D-melibiose, β-hydroxybutyric acid, propionic acid, L-pyroglutamic acid, uridine 5'-monophosphate, Tween 60, methyl α-D-galactoside, sedoheptulosan, γ-hydroxybutyric acid, pyruvic acid, L-serine, fructose 6-phosphate, D-gluconic acid, methyl β-D-galactoside, D-sorbitol, p -hydroxyphenyl acetic acid, succinamic acid, putrescine, glucose 1-phosphate, stachyose, α-ketoglutaric acid, succinic acid, 2,3-butanediol, glucose 6-phosphate, amygdalin, m -inositol, methyl α-D-glucoside, sucrose, α-ketovaleric acid, N -acetyl L-glutamic acid and DL-α-glycerol phosphate. +, Positive; ‾, negative; W, weak. Table 1. Phenotypic properties that differentiate the novel isolate from Erysipelothrix strains, as determined by the API 32 STREPT and Biolog GP microplate panels

Characteristic	1	2	3	4	5	6
API STREPT:
β-Glucosidase	+	‾	‾	‾	‾	+
Alkaline phosphatase	‾	‾	‾	‾	‾	+
Ribose (acid)	W	‾	‾	‾	‾	+
Lactose (acid)	‾	+	+	+	+	‾
Trehalose (acid)	+	‾	‾	‾	‾	‾
N -Acetyl-β-glucosaminidase	+	+	‾	W	+	+
β-Mannosidase	W	‾	‾	‾	‾	‾
Utilization of (Biolog GP Microplate panel):
L-Arabinose	‾	+	+	+	+	W
N -Acetyl-D-mannosamine	‾	+	+	+	+	+
Arbutin	+	‾	‾	‾	‾	‾
Cellobiose	+	‾	‾	‾	‾	‾
D-Fructose	‾	+	+	+	+	+
D-Galactose	‾	+	+	+	+	+
Gentiobiose	+	‾	‾	‾	‾	‾
α-D-Lactose	‾	+	+	+	+	‾
D-Mannose	‾	+	+	W	+	‾
3-Methyl glucose	‾	‾	‾	‾	+	‾
D-Psicose	‾	+	+	+	+	+
D-Ribose	W	‾	+	+	+	+
Salicin	+	‾	‾	‾	‾	‾
D-Trehalose	+	‾	‾	‾	‾	‾
Xylose	‾	‾	+	+	+	W
Glycerol	+	‾	‾	‾	‾	‾

bridge that consists of GlyRL-LysRL-Lys. C16: 0, C18: 19 cis and C18: 0 are predominant fatty acids. Aerobic to faculta- tively anaerobic. Chemoorganotrophic; metabolism is respiratory and weakly fermentative. Acid, but no gas, is produced from glucose and other carbohydrates. DNA G+C content is 36–40 % ( HPLC , T m, Bd). Some strains are pathogenic for mammals and birds. The 16S rRNA gene-directed primer pair (forward, 5'-TGATGCCATA- GAAACTGGTA-3'; reverse, 5'-CTGTATCCGCCATAAC- TA-3') specifically amplifies the DNA of members of the genus Erysipelothrix . Belongs phylogenetically to the Firmicutes . Type genus is Erysipelothrix (Migula 1900) Buchanan 1918, 55.