Descriptions of two new species of Hemileucinae (Lepidoptera: Saturniidae) from the region of Muzo in Colombia-evidence from morphology and DNA barcodes Author Decaëns, Thibaud Laboratoire d'Écologie, ECODIV - EA 1293, Federation de Recherche SCALE, U. F. R. Sciences et Techniques, Université de Rouen, F- 76821 Mont Saint Aignan cedex, France. E-mail: thibaud. decaens @ univ-rouen. fr Muséum National d’Histoire Naturelle, Département Systématique et Evolution, USM 602, case postale n ° 50 (Entomologie), F- 75231 Paris cedex 05 Biodiversity Institute of Ontario, University of Guelph, 579 Gordon Street, N 1 G 2 W 1, Guelph, Ontario, Canada. E-mail: rrougeri @ uoguelph. ca Author Rougerie, Rodolphe text Zootaxa 2008 2008-11-28 1944 1 34 52 https://biotaxa.org/Zootaxa/article/view/zootaxa.1944.1.2 journal article 10.11646/zootaxa.1944.1.2 1175­5334 5231243 Cerodirphia zulemae Decaëns and Rougerie , new species Figs. 16–19 , 24, 28 , 32–36 and 52–54 . Type material. Holotype , male: Colombia , Boyacá department , Municipio de Quipama , Vereda Caviche , alt. 1500 m . a.s.l., 1–3.xii.2002 ; attracted to UV light, D. Bonilla and G. Lecourt leg.; genital prep. TD#141; barcode sequence BC-Dec0010/ SATWA047-06 . Deposited in INCN . Paratypes ( Figs. 17–19 ): 4 males ( 1 in collection of R . Rougerie, 2 in collection of T . Decaëns, 1 in MNHN ) and 2 female (one designated as allotype and deposited in INCN , the other in the collection of T . Decaëns), same data as the holotype; 3 males have barcode sequences attached on BOLD : BC-Dec0008/ SATWA045-06 , BC-Dec0009/ SATWA046-06 , BD-Dec0491/ STDA481-07 . TABLE 4. Diagnostic characters to distinguish L. bonillensis n. sp. from closely related species. FW = forewings; HW = hindwings (traits in this table are relative, not absolute)..

L. bonillensis n.sp. (Figs. 3–4, 7–9)	L. acutissima (Figs. 5, 10–12)	L. newmani (Figs. 6, 13–15)
FW shape	Less elongated	More elongated	More elongated
FW postmedial line	Straight and sub-apical	Curved and sub-apical	Curved and pre-apical
FW ante- and post- medial lines	Large, dark, bordered with a nar- row yellow strip	Large, dark, with narrow yel- low strip	Thin, with larger yellow strip
HW eyespot	Large diameter	Small diameter	Small diameter
HW peri-ocellar ring	Large	Narrow	Narrow
HW verso pupil	Absent	Present	Present
HW verso postme- dial line	Absent	Continue	Present but interrupted
Male genitalia saccus	Large, moderately pointed	Thin, pointed	Large, moderately pointed
Male genitalia costal lobe of the valves	Large, rounded and curved toward median axis	Medium-sized, squared and curved backward	Large, rounded and curved backward
Male genitalia phallus	Bulbus ejaculatorius crosier- shaped, three times as long as the aedeagus	Bulbus ejaculatorius apically rounded, one and a half time as long as the aedeagus	Bulbus ejaculatorius apically rounded, three times as long as the aedeagus

Diagnosis. Within the genus, Cerodirphia zulemae n. sp. belongs to the Cerodirphia speciosa Cramer (1777) group ( Fig. 22 , 47–51 ), which is characterized by the vivid pink background colour of the wings and by the presence of a characteristic “Y”-shaped discal mark on the forewing. Based on the structure of the male genitalia ( Figs. 32–36 ), C. zulemae appears to be closely related to Cerodirphia brunnea Draudt 1930 ( Figs. 23 , 42–46 ) and Cerodirphia apunctata Dias & Lemaire 1991 ( Fig. 20–21 , 37–41 , Table 5 ) ( Lemaire 2002 ). It can be distinguished easily from C. brunnea by its pink rather than brown ground colour, the more elongated hindwing, the absence of medial lines on both wings ( Figs. 16, 18–19, 23 ), and the more rounded general shape of the male genitalia ( Figs. 32–33, 42–43 ). It is difficult to separate C. zulemae from C. apunctata using only external characters. The somewhat sharper apex and more elongated hindwing ( Figs. 16, 18–21 ) are in fact subtle characters that may represent intra-specific variation ( Lemaire 2002 ). The strongest diagnostic characters thus are to be found in the male genitalia morphology (see discussion). C. zulemae differs mainly from C. apunctata by the morphology of the sclerites of the eighth abdominal segment ( Figs. 24–25, 28–29 ) and the larger and more sclerotized cornuti of the vesica ( Figs. 35–36, 40–41 ). Description. Male ( Figs. 16, 18–19 ). Wingspan: 71–80 mm . Head : orange, labial palpi of the same colour, antennae light orange. Thorax : With orange erected scales; leg vestiture a mixture of short appressed light brown scales and hair-like orange scales. Forewing length 37–42 mm , elongated with straight outer margin, rounded apex; ground colour light pink brown, with a clear pink fringe on outer margin; venation sustained by a thin web of brown scales. Classical “Y”-shaped mark extending on veins from the origin of CuA1 and bifurcating to the apical corner of the discal cell and to the first third of vein M3; this mark white, heavily sustained with dark grey scales along vein M3 and the transverse discal vein between M2 and M3. Hindwing with same colour pattern as forewing, with a wider extension of the areas covered by pink scales on the dorsal side; zone between vein Sc+R and the costal margin almost entirely covered with white scales on both sides of the wing; discal markings absent; baso–median dorsal area extensively covered by brownish orange hair–like scales. Ventral side same colour as dorsal with similar patterns except for the discal spot of the forewings rep- resented by a small rectangular area of light scales. Abdomen : Dorsal side black with white intersegmental transversal strips; anal tuft and ventral side orange. Posterior margin of eighth sternum ( Fig. 24 ) enlarged and highly sclerotized, minutely dentate on its external quarter and showing a small smooth concavity on each side of a short median spine; eighth tergum ( Fig. 28 ) triangular, its posterior margin tapering to a small and thin process. FIGURES 16–23. Wing patterns of Cerodirphia zulemae n. sp. and closely related species. 16. Male Holotype, dorsal vew; 17. Female Allotype, dorsal view; 18. Male paratype, dorsal view; 19. Idem, ventral view; 20. Male of Cerodirphia apunctata , dorsal view (French Guyana, crossroad RN2-Cacao, 25 i 1999, leg. R. Rougerie, ex larva–emerged 30 vii 1999, genitalia prep. S–RR#57); 21. Male of C. apunctata , dorsal view (Venezuela, Bolivar, road El Dorado – Sta Elena, km18, la Escalera, 1400m asl, 7–20 xi 1990, leg. P. Bleuzen, genitalia prep. C. Lemaire # 5718). 22. Male of Cerodirphia speciosa , dorsal view (French Guyana, Cayenne, i 1972, leg. J.J. de Granville, genitalia prep RR#295); 23. Male of Cerodirphia brunnea , dorsal view (Bolivia, La Paz, Nor Yungas, Carrasco, 1450m asl, xi 1990, leg. T. Decaëns & G. Lecourt). FIGURES 24–31. Genitalia patterns of Cerodirphia zulemae n. sp. and closely related species. 24. 7 th tergite of the Holotype of C. zulemae , dorsal view (genitalia prep. TD#141); 25. Same structure, Cerodirphia apunctata (genitalia prep. S–RR#57); 26. Same structure, Cerodirphia brunnea (genitalia prep. TD#174); 27. Same structure, Cerodirphia speciosa (genitalia prep. RR#295); 28. 7 th sternite of the Holotype of C. zulemae , ventral view; 29. Same structure, C. apunctata ; 30. Same structure, C. brunnea ; 31. Same structure, C. speciosa . Male genitalia ( Figs. 32–36 ). Uncus ( Figs. 32–34 ) narrow, its posterior half setose, downcurved and highly sclerotized; apical part narrow then widening as a small plate with a curving rim. Gnathos present and merged with the transtilla, the median plate of which is broad, heavily sclerotized and anteriorly connected to the juxta ( Fig. 33 ); its lateral arms merged basally with the valvae, highly setose, oriented backward and resembling small valvae. Valva short and thick ( Fig. 34 ), produced as small tapering lobe at the apex; the base merged with the juxta ( Fig. 33 ), the latter forming a sclerotized ring fused with the surrounding structures. Saccus short, anterior margin rounded. Aedeagus ( Figs. 35–36 ) short, posterior part longitudinally divided in two highly sclerotized parts; caecum penis enlarged. Vesica evaginating ventrally at the longitudinal division of aedeagus, bearing two cornuti: a large and highly sclerotized square tooth and a very small apical spike. Female ( Fig. 17 ). Wingspan: 98 mm . Very similar to male but larger. Head : Dark yellow–orange, labial palpi concolourour with head, antennae light orange. Thorax : Orange brown; legs light orange. Forewing length 55 mm , elongate, with slightly convex border, rounded apex; above ground colour pink, scattered with dark orange brown scales, the pink colour being more vivid near the margins; venation marked with brown scales; discal markings as described for the male; proximal area covered with pink–orange hair–like scales near the anal border. Hindwing with same colour pattern but devoid of discal mark and with a wider extension of the pink areas. Ventral side with similar colour patterns as the dorsal one; forewing discal mark represented by a grey strip bordered of lighter scales; hindwings with a rectangular discal spot of light scales; area delimited by Sc+R and the costal margin dark brown externally bordered with a fringe of white hair–like scales. Abdomen : Dorsal side black with white intersegmental transversal strips; anal tuft and ventral side dark orange brown. FIGURES 32–51. Male genitalia patterns of Cerodirphia zulemae n. sp. and closely related species. 32. Genitalia of the Holotype of C. zulemae , dorsal view (genitalia prep. TD#141); 33. Idem, ventral view; 34. Idem, lateral view; 35. Idem, lateral view of the edeagus; 36. idem, dorsal view of the edeagus; 37. Genitalia of Cerodirphia apunctata , dorsal view (genitalia prep. S–RR#57); 38. Idem, ventral view; 39. Idem, lateral view; 40. Idem, lateral view of the edeagus; 41. idem, dorsal view of the edeagus; 42. Genitalia of Cerodirphia brunnea , dorsal view (genitalia prep. TD#174); 43. Idem, ventral view; 44. Idem, lateral view; 45. Idem, lateral view of the edeagus; 46. idem, dorsal view of the edeagus; 47. Genitalia of Cerodirphia speciosa , dorsal view (genitalia prep. RR#295); 48. Idem, ventral view; 49. Idem, lateral view; 50. Idem, lateral view of the edeagus; 51. idem, dorsal view of the edeagus. Female genitalia ( Fig. 52–54 ). Papillae anales setose; anterior and posterior apophyses subequal in length. Vaginal plate laterally connected to the eighth tergum, broad, with merged lamella postvaginalis; the latter ( Fig. 53 ) composed of a pair of small sclerotized lobes and a median shield-like ( Lemaire 2002: 750 ) structure bearing a small triangular notch. Tergum eight medially interrupted. Ostium bursae barely visible. Ductus bursae short, broad and heavily sclerotized. Corpus bursae rounded, its surface smooth. Distribution and biology. This species is known only from the type locality at an elevation between 1500 and 2000 m a.s.l. As for L. bonillensis , its exact relationship with the three biogeographical regions that contribute to the Saturniidae fauna of the mountains of Muzo ( Decaëns et al. 2007 ) will require more data to be defined precisely. The presence of both species on the other side of the Oriental Cordillera (which goes down to the Eastern Plains) also requires investigation. For example, a female of Cerodirphia from the Amazonian Piedmont of the Caqueta department ( Racheli & Vinciguerra 2005 ) should be compared carefully to C. zulemae . Immature stages and biology are unknown Etymology. This species is dedicated to the wife of the senior author, acknowledging her patience and collaboration during all the collecting trips made by D. Bonilla, L. D. Ramirez and T. Decaëns in Colombia .