Paraeutypella guizhouensis gen. et sp. nov. and Diatrypella longiasca sp. nov. (Diatrypaceae) from China Author Dissanayake, Lakmali S. Engineering Research Centre of the Utilization for Characteristic Bio-Pharmaceutical Resources in Southwest, Ministry of Education, Guizhou University, Guiyang, Guizhou Province 550025, China Author Wijayawardene, Nalin N. https://orcid.org/0000-0003-0522-5498 Center for Yunnan Plateau Biological Resources Protection and Utilization, College of Biological Resource and Food Engineering, Qujing Normal University, Qujing, Yunnan 655011, China & State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550014, China Author Dayarathne, Monika C. Department of Plant Pathology, Agriculture College, Guizhou University, Guiyang, Guizhou Province, 550025, China Author Samarakoon, Milan C. Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai, 57100, Thailand Author Dai, Dong-Qin Center for Yunnan Plateau Biological Resources Protection and Utilization, College of Biological Resource and Food Engineering, Qujing Normal University, Qujing, Yunnan 655011, China Author Hyde, Kevin D. Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai, 57100, Thailand Author Kang, Ji-Chuan Engineering Research Centre of the Utilization for Characteristic Bio-Pharmaceutical Resources in Southwest, Ministry of Education, Guizhou University, Guiyang, Guizhou Province 550025, China jckang@gzu.edu.cn text Biodiversity Data Journal 2021 2021-03-26 9 63864 63864 http://dx.doi.org/10.3897/BDJ.9.e63864 journal article http://dx.doi.org/10.3897/BDJ.9.e63864 1314-2828-9-e63864 85D1EDC701FF5024B7B6F37CCC1A5DC2 Paraeutypella citricola L.S. Dissan., Wijayaw., J.C. Kang & K.D. Hyde comb. nov. Paraeutypella citricola Speg., in Anales del Museo Nacional de Buenos Aires 6: 245, (1898) = Eutypella citricola Syd. & P. Syd., Hedwigia 49: 80 (1909), nom. illegit., Art. 53.1 Materials Type status: Holotype . Record Level: institutionID: LPS-2120 Type status: Paratype . Occurrence: recordedBy: Nalin N. Wijayawardene ; Taxon: kingdom: Fungi; phylum: Ascomycota; class: Sordariomycetes; order: Xylariales; family: Diatrypaceae; genus: Paraeutypella; specificEpithet: citricola; Location: country: China ; county: Guiyang; locationAccordingTo: Guizhou University Garden (South); Identification: identifiedBy: L.S.Dissanayake ; Event: year: 2019; month: October; day: 5; habitat: on a dead branch of Acer sp.; Record Level: type: paratype ; institutionID: HMAS 290660, HMAS 290659; collectionID: culture KUMCC 20-0024, KUMCC 20-0023; institutionCode: Chinese Academy of Science , Kunming and Chinese Academy of Science Herbarium ; collectionCode: Kunming Institute of Botany Culture Collection Description Saprobic on dead twigs of Acer palmatum (Fig. 4 ). Sexual morph : Stromata immersed in bark of dead branches, erumpent, solitary or aggregated, circular to irregular in shape, superficial, carbonaceous. Ascomata 410-430 x 430-470 μm (x̅ = 420 x 450 µm , n = 10), perithecial, with groups of 4-6 perithecia arranged in a valsoid configuration, black, subglobose, clustered, immersed in ascostroma with ostiolar neck. Necks 360-390 μm long (x̅ = 375 µm , n= 10), papillate, sulcate, central ostiolar canal filled with paraphyses. Peridium 27-40 μm wide, composed of two layers of textura angularis; inner layer cells hyaline, outer layer cells dark brown to black. Hamathecium composed of 3-7 μm wide (x̅ = 5 µm , n= 10), hyaline, paraphyses arising from base of perithecia, composed of long, narrow, unbranched, septate, guttulate, narrowing and apically truncate. Asci 70-75 x 5-8 μm (x̅ = 72.5 x 6.5 μm , n = 20), 8-spored, unitunicate, thin-walled, clavate to cylindrical clavate, long pedicellate (40-50 μm ), J- apical ring. Ascospores 7-10 x 2-3 μm (x̅ = 8.5 x 2.5 μm , n = 30), overlapping biseriate, allantoid, hyaline to light brown, smooth, aseptate, usually with guttules. Asexual morph : Undetermined. Culture characteristics - Colonies on PDA, reaching 21 mm diam. after 2 weeks at 20-25oC, medium dense, circular to slightly irregular, slightly raised, cottony surface; colony from above: at first white, becoming buff; from below: yellowish-white at margin, yellow to brown at centre; mycelium yellowish. Notes Eutypella citricola was described by Spegazzini (1898) from Citrus in Argentina and has since been reported to cause dieback on various woody plants in warm temperate and tropical regions ( Farr and Rossman 2020 ). Eutypella citricola strains have previously been isolated from hosts such as Citrus limon , C. sinensis , C. paradisi , Salix spp., Schinus molle , Ulmus procera and Vitis vinifera ( Trouillas et al. 2011 , Mehrabi et al. 2016 ). In our study, we provide additional information for P. citricola from dead stems of Acer ( Sapindaceae ) in China. In morphology, our collection (HMAS 290660) resembles Eutypella , thus having pustulate stromata with stout, converging ostiolar necks and asci with eight spores. According to phylogenetic analysis, KUMCC 20-0024 closely groups with a collection of E. citricola (IRAN 2349C), which was collected on dead branches of Salix sp. ( Salicaceae ) in Gilan Province, Iran ( Mehrabi et al. 2016 ) (Fig. 1 ). However, the IRAN 2349C strain is slightly different from our strain in having stromata with groups of 6-25 perithecia in a valsoid configuration and short ostiolar necks (100-300 µm ), while our collection comprises stromata with groups of 4-6 perithecia in a valsoid configuration with a longer neck (356-385 μm ). Based on phylogenetic analysis, both strains grouped in Paraeutypella sensu stricto (Fig. 1 ). Hence, the name Eutypella citricola is placed in Paraeutypella as P. citricola . Paraeutypella guizhouensis , the type of Paraeutypella , morphologically resembles P. citricola both having immersed stromata, perithecia each with a long ostiolar neck and allantoid, aseptate ascospores with an oil droplet at each end. However, Paraeutypella citricola differs from P. guizhouensis by the number of perithecia within a stroma (4-6 vs. 6-12). A comparison of the ITS 1.0% (6/576) and β-tubulin 1.2% (5/406) between KUMCC 20-0024 and IRAN 2340C, ITS 1.0% (6/576) and β-tubulin 1.0% (5/406) between KUMCC 20-0024 and HVGRF01, HVVIT07 has been made.