Pyrenochaetopsis kuksensis (Pyrenochaetopsidaceae), a new species associated with an ornamental boxwood in the Czech Republic Author Špetík, Milan 0000-0001-7659-8852 MENDELEUM - Institute of Genetics, Mendel University in Brno, Valticka 334, 691 44, Lednice, Czech Republic. & milan. spetik @ mendelu. cz; https: // orcid. org / 0000 - 0001 - 7659 - 8852 milan.spetik@mendelu.cz Author Berraf-Tebbal, Akila 0000-0001-8517-8542 MENDELEUM - Institute of Genetics, Mendel University in Brno, Valticka 334, 691 44, Lednice, Czech Republic. & qqberraf @ mendelu. cz; https: // orcid. org / 0000 - 0001 - 8517 - 8542 qqberraf@mendelu.cz Author Pokluda, Robert 0000-0003-0492-6401 Department of Vegetable Growing, Mendel University in Brno, Valticka 334, 691 44, Lednice na Morave, Czech Republic. & robert. pokluda @ mendelu. cz; https: // orcid. org / 0000 - 0003 - 0492 - 6401 robert.pokluda@mendelu.cz Author Eichmeier, Aleš 0000-0001-7358-3903 MENDELEUM - Institute of Genetics, Mendel University in Brno, Valticka 334, 691 44, Lednice, Czech Republic. & ales. eichmeier @ mendelu. cz; https: // orcid. org / 0000 - 0001 - 7358 - 3903 ales.eichmeier@mendelu.cz text Phytotaxa 2021 2021-04-30 498 3 177 185 http://dx.doi.org/10.11646/phytotaxa.498.3.3 journal article 10.11646/phytotaxa.498.3.3 1179-3163 5424232 Pyrenochaetopsis kuksensis Spetik, Eichmeier & Berraf-Tebbal , sp. nov . ( Fig.2 ) Mycobank number: MB835803 Type:— CZECH REPUBLIC , Nachod : Kuks , castle garden ( 50°23’49.0”N 15°53’21.7” E ), isolated from the wood of Buxus sempervirens , September 2018 , M . Spetik, Holotype : BRNU 673828 , isotype BRNU 673829 , ex-type living culture CBS 146534 = MEND-F-57 Saprobic on dead wood of Buxus sempervirens . Asexual morph: Conidiomata pycnidial, brown, solitary or aggregated, semi-immersed, globose to ovoid, setose, ostiolate, unilocula. Conidiogenous cells phialidic, hyaline, discrete and integrated in septate. Conidia hyaline, aseptate, cylindrical to allantoid, slightly guttulate, (2.5–)2.9–4(–3) × (1–)1.4–2(–1.5) µm mean 3 ± 0.4 × 1.4 ± 0.2 µm. Colonies on PDA reaching 32 mm diam at 25 ° C after 10 d, margin regular, floccose, white in outer ring, changing to salmon towards the centre of the colony; reverse white. On MEA reaching 36.5 mm diam after 10 d, margin regular, floccose, erumpent, white in outer ring, changing to salmon towards the centre of the colony; reverse white. On OA reaching 44 mm diam after 10 d, margin regular, floccose, white in outer ring, grey-olivaceous, white in center; reverse grey. No growth at 10° and 37° C was observed. Etymology:— named after Kuks, where the taxon was collected. Additional specimens examined:— CZECH REPUBLIC , Nachod : Kuks , castle garden, isolated from the wood of Buxus sempervirens , September 2018 , M . Spetik, living culture MEND-F-58 .