A new species of Diadumene (Actiniaria: Diadumenidae) from the subtropical coast of Brazil Author Beneti, Julia S. Author Stampar, Sérgio N. Author Maronna, Maximiliano M. Author Morandini, André Carrara Author Silveira, Fábio Lang Da text Zootaxa 2015 4021 1 156 168 journal article 10.11646/zootaxa.4021.1.6 ebb70852-4885-4768-94f3-dd3d2df35c99 1175-5326 232124 CDBF1C6B-C24E-4321-8699-F390339D0F30 Diadumene paranaensis n. sp. ( Figures 2–4 ) Type material. Holotype specimen : MZUSP 1530, polyp without reproductive tissue, 6 mm height, 3 mm width, collected 25 October 2010 , at the Yacht Club of Paranaguá, Paranaguá Bay, on mussel, depth of 1-2 m , salinity 26 psu, temperature 20° C. Preserved in 4% FSW. Paratype specimens : MZUSP 1531, one specimen without reproductive tissue, same data as holotype . MZUSP 1532, six specimens without reproductive tissue, same data as holotype . MZUSP 1534, three specimens without reproductive tissue, collected 23 October 2009 , preserved two days after collection in 4% FSW. Additional specimens : MZUSP 1533, one specimen without reproductive tissue, collected 21 August 2009 , preserved two days after collection in 4% FSW. MZUSP 1535, three specimens without reproductive tissue, collected 23 October 2009 , preserved two days after collection in 100% ethanol. MZUSP 1536, three specimens without reproductive tissue, collected 22 February 2010 , preserved two days after collection in 4% FSW. MZUSP 1537, six specimens without reproductive tissue, collected 23 August 2010 , preserved two days after collection in 4% FSW. MZUSP 1909, transverse histological sections of specimen collected 23 October 2009 . MZUSP 1910, longitudinal histological sections of specimen collected 23 October 2009 . MZUSP 1911, transverse histological sections of specimen collected 22 February 2010 . MZUSP 1912, longitudinal histological sections of specimen collected 22 February 2010 . MZUSP 1913, transverse histological sections of specimen collected 24 May 2010 . MZUSP 1914, longitudinal histological sections of specimen collected 24 May 2010 . AMNH 6121, six specimens without reproductive tissue, collected 24 May 2010 , preserved one week after collection in 4% FSW. AMNH 6122, seven specimens without reproductive tissue, collected 24 May 2010 , preserved one week after collection in 4% FSW. AMNH 6123, eleven specimens without reproductive tissue, collected 21 August 2010 , preserved three days after collection in 4% FSW. AMNH 6124, eleven specimens without reproductive tissue, collected 21 August 2010 , preserved three days after collection in 4% FSW. AMNH 6125, longitudinal histological sections of specimen collected 22 February 2010 . AMNH 6126, longitudinal histological sections of specimen collected 21 August 2010 . AMNH 6127, transverse histological sections of specimen collected 24 May 2010 . AMNH 6128, transverse histological sections of specimen collected 21 August 2010 . TABLE 3. Size and distribution of cnidae of Diadumene paranaensis n. sp. Measurements in micrometers (µm), expressed as range of minimum and maximum values, mean size and standard deviation. L/W = mean ratio of length and width of the capsules measured; N = number of capsules measured; P = specimens that showed a particular type of cnida/ specimens analyzed; F = frequency of each type of cnida (+ + + = very common; + + = common; + = not common).

Tissue / Type of cnida	Range of length x width	Mean±SD	L/W	N	P	F
Tentacles
Spirocyst	10.28–17.85 x 2.65–4.72	14.54±2.89 x 3.28±1.01	4.52	50	2/8	+ + +
Atrich	25.8–34.2 x 7.2–11.4	31.02±2.14 x 9.94±1.09	3.22	30	1/8	+ +
Holotrich	21.6–27.6 x 4.8–7.2	25.34±2.01 x 6.16±0.76	4.28	40	2/8	+ +
Basitrich	7.2–21.2 x 1.2–3.6	14.03±2.72 x 2.33±0.44	6.21	80	8/8	+ + +
Microbasic p-amastigophore	19.8–26.4 x 3.6–6.0	22.75±1,58 x 4.68±0.58	4.94	60	8/8	+ + +
Macrobasic p-amastigophore	36.0–46.2 x 6.0–8.4	41.01±2.46 x 7.29±0.66	5.68	40	8/8	+ +
Pharynx
Basitrich	16.8–21.28 x 1.2–3.7	19.67±1.07 x 2.67±0.71	8.04	30	5/5	+
Microbasic p-amastigophore	15–22.8 x 3–4.2	17.84±1.65 x 3.64±0.35	4.94	39	5/5	+ +
Capitulum
Basitrich	9.6–13.5 x 1.2–2.4	10.85±1.38 x 2.04±0.53	5.63	26	5/5	+
Microbasic p-amastigophore	15.1–23.4 x 2.4–4.8	17.8±2.04 x 3.60±0.74	5.11	18	5/5	+
Scapus
Basitrich	15.2–20.7 x 2.4–4.2	18.14±1.22 x 3.04±0.34	6.03	59	5/5	+ +
Microbasic p-amastigophore	9.9–15.6 x 3.0–4.8	12.78±1.65 x 4.05±0.53	3.23	14	5/5	+
Filament
Basitrich	10.8–19.2 x 1.2–3.0	15.50±2.19 x1.86±0.51	8.10	39	6/6	+ + +
Microbasic p-amastigophore	39.0–51.6 x 6.0–8.4	46.46±1.28 x 7.07±0.51	6.63	37	6/6	+ +
Microbasic p-amastigophore	7.2–12.9 x 2.8–4.8	11.20±2.09 x 4.06±0.78	2.81	62	6/6	+ +
Microbasic p-amastigophore	12.0–22.8 x 3.0–4.8	16.16±2.33 x 3.77±0.43	4.34	40	6/6	+ +
Acontia
Basitrich	10.2–19.2 x 1.2–3.6	15.50±2.01 x 1.96±0.52	8.35	71	8/8	+ + +
Microbasic p-amastigophore	35.9–57.5 x 5.1–7.4	43.61±4.17 x 6.07±0.64	7.17	60	8/8	+ +
Microbasic p-mastigophore	41.1–57.0 x 5.4–8.4	46.59±3.34 x 6.56±0.78	7.31	85	8/8	+
Microbasic p-mastigophore	19.2–26.4 x 3.0–6.0	22.42±2.71 x 4.42±0.82	5.21	18	2/8	+

Comparative material : Diadumene cincta MNRJ 3326, 8 specimens , collected 21 November 1981 , Ouwerkerk, Province of Zeeland, The Netherlands , preserved in formaldehyde solution, leg. det. M.S.S. Lavayele. Diadumene kameruniensis RMNH . COEL 19755, 2 specimens , collection date unknown, Nigeria , Port Harcourt, preserved in formaldehyde solution. Diadumene kameruniensis RMNH . COEL 32120, data unknown, preserved in ethanol, det. Carlgren. Diadumene leucolena RMNH . COEL 11472, 3 specimens , collected 20 August 1973 , Curaçao , Spaanse water, leg. det. J.C. den Hartog. Diadumene lineata RMNH . COEL 23784, 7 specimens , collected 13 October 1990 , Veerse Meer, The Netherlands , preserved in formaldehyde solution, leg. M. Faase, det. J.C. den Hartog (1995). Type locality. Yacht Club of Paranaguá, Paranaguá Bay, Paraná state, southern Brazil ( 25°30'53"S , 48°29'58"W ). Etymology . The specific name paranaensis refers to the state of Paraná, in Brazil , the type locality of the species. Diagnosis . Clonal species of Diadumene ; polyp with cinclides arranged in longitudinal rows, macrobasic pamastigophores in the tentacles, and microbasic p-amastigophores in the acontia.