A new species and a new record of the genus Phaeophyscia Moberg (Lecanorales, Physciaceae) from Pakistan supported by phenotypic and molecular phylogenetic analyses Author Niazi, Abdul Rehman Fungal Biology and Systematics Laboratory, Institute of Botany, University of the Punjab, Quaid-e-Azam Campus- 54590, Lahore (Pakistan) Author Afshan, Najam-ul-Sehar Fungal Biology and Systematics Laboratory, Institute of Botany, University of the Punjab, Quaid-e-Azam Campus- 54590, Lahore (Pakistan) Author Naseer, Arooj Fungal Biology and Systematics Laboratory, Institute of Botany, University of the Punjab, Quaid-e-Azam Campus- 54590, Lahore (Pakistan) Author Nadeem, Muhammad Fungal Biology and Systematics Laboratory, Institute of Botany, University of the Punjab, Quaid-e-Azam Campus- 54590, Lahore (Pakistan) Author Iftikhar, Fatima Fungal Biology and Systematics Laboratory, Institute of Botany, University of the Punjab, Quaid-e-Azam Campus- 54590, Lahore (Pakistan) Author Fayyaz, Iram Fungal Biology and Systematics Laboratory, Institute of Botany, University of the Punjab, Quaid-e-Azam Campus- 54590, Lahore (Pakistan) iramfiaz04@gmail.com Author Ashraf, Asma Fungal Biology and Systematics Laboratory, Institute of Botany, University of the Punjab, Quaid-e-Azam Campus- 54590, Lahore (Pakistan) Author Imtiaz, Sawera Fungal Biology and Systematics Laboratory, Institute of Botany, University of the Punjab, Quaid-e-Azam Campus- 54590, Lahore (Pakistan) Author Fatima, Shahzadi Qamar Fungal Biology and Systematics Laboratory, Institute of Botany, University of the Punjab, Quaid-e-Azam Campus- 54590, Lahore (Pakistan) Author Khalid, Abdul Nasir Fungal Biology and Systematics Laboratory, Institute of Botany, University of the Punjab, Quaid-e-Azam Campus- 54590, Lahore (Pakistan) text Cryptogamie, Mycologie 2023 2023-04-19 20 4 51 59 http://dx.doi.org/10.11646/zootaxa.4878.3.2 journal article 255745 10.5252/cryptogamie-mycologie2023v44a4 e40386a3-58a0-4a6b-9e6d-e4ebcabfb8e1 1776-100X 7867201 Phaeophyscia microspora Aptroot & Schumm ( Fig. 3 ) Virtuelles Herbarium de Flechtengattungen Hyperphyscia , Paeophyscia , Physcia und Physconia : 193 ( Schumm & Aptroot 2019 ). SPECIMENS EXAMINED . — Pakistan . Margalla Hills, Islamabad, 1604 m alt., on tree bark, 33°41’35”N , 73°03’50”E , 09.IV.2019 , A. Ashraf, K. Habib & M. Usman, T5-02 (LAH[LAH37622]; GenBank[OQ024193]); Azad Jammu and Kashmir, District Bagh, 33.9259°N , 73.7810°E , 4734 m alt., on tree bark, 10.XI.2021 , A. Naseer, B-04 (LAH[LAH37624]; GenBank[OQ073895]); Azad Jammu and Kashmir, District Bagh, 33°55’33.24”N , 73°46’51.6”E , 4734 m alt., on tree bark, 12.XI.2021 , A. Naseer, B-05 (LAH[LAH37625]; GenBank[OQ073896]); Khyber Pakhtunkhwa , Kaghan Valley , Shogran , 34°64’N , 73°46’E , 2362 m alt., on bark , 24.VIII.2021 , A. R. Niazi & M. Nadeem , SN-1 ( LAH [ LAH37618 ]; GenBank[ OP933725 ]); Khyber Pakhtunkhwa , Kaghan Valley , Siri Paya , 33°71’N , 78°08’E , 3000 m alt., on tree bark , 26.VIII.2022 , A. R. Niazi & M. Nadeem , BK-14 ( LAH [ LAH37617 ]; GenBank[ OP933722 ]). CHEMISTRY . — Thallus K-, C-, KC-, P-; no lichen substance detected by TLC. HABITAT AND DISTRIBUTION . — Pakistani collections of this species are from humid to moist temperate, coniferous forest and scrub forest. The specimens were found on tree bark. The maximum daily temperature of the Himalaya region varies around 30-32°C during the summer, the average winter temperature is 4°C, while Margala Hills have an average maximum temperature of 34.3°C and minimum of 3.4°C with an average rainfall of 1200 mm per year. DESCRIPTION Thallus Foliose, epiphloeodal, rust grey to pale grey, remaining unchanged when wet, loosely attached to substratum, very variable in size, 2-4 cm in diam. Lobes Densely, dichotomously or irregularly branched, without pruina, lobe tips creamy white, flat to strongly convex, imbricate, usually upturned near the tips, 0.5-1.5 mm wide. Soralia and isidia Absent. Cortex Dark brown, paraplectenchymatous, 18-23 µm thick. Algal layer 28-34 µm thick. Photobiont cells Globose, 8-13 µm in diam. Medulla White. FIG . 3. — Phaeophyscia microspora Aptroot & Schumm : A , foliose thallus of type specimen (holo-, LAH[LAH37622]); B , apothecia; C , lobe with rhizines; D , section of an apothecium; E , ascus; F , ascospores. Scale bars: A, 1 cm; B, 3 mm; C, 1.5 mm; D, 80 μm; E, 13 μm; F, 8 μm. Lower surface Black, usually becoming pale to dark grey towards lobe tips, rhizinate. Rhizines Dense, black, simple, 1.3-2.5 mm long. Lower cortex Paraplectenchymatous, dark brown, 18-30 µm thick. Apothecia Abundant, usually present, 1.5-3 mm in diam., stipitate to very shortly stipitate, the margin entire or becoming regularly crenate, with prominent cortical hairs, epruinose or slightly pruinose. Disc Charcoal black, shiny, flat to strongly concave or sometimes convex. Margins Prominent, creamy to pale white. Epihymenium Light brown to orange, 18-24 µm. Hymenium Hyaline to creamy, 70-90 µm. Hypothecium Creamy white, 22-28 µm. Ascus Cylindrical to clavate, 45-55 ×12-14 µm. Ascospores Dark brown, ellipsoid, Physcia - type , 16-20 ×7.5-9 µm. Paraphyses Hyaline, branched and anastomosing, 1.7-2.8µm thick, wider at the apex, 3-4 µm thick. Pycnidia Not found.