Triplectides Kolenati (Trichoptera: Leptoceridae) from Brazil: A new species, new records and an identification key Author Desidério, Gleison Robson 63EE23DE-6125-4167-A3AA-27E2A9829283 Instituto Nacional de Pesquisas da Amazônia (INPA), Coordenação de Pós-Graduação (COPOG), Divisão do Curso em Entomologia (DiEnt), Coordenação de Biodiversidade (CoBio), Manaus, Amazonas, Brazil. gleysonbio@gmail.com Author Pes, Ana Maria 03D29AFD-A7EE-49DA-9740-7FA0F34932AF Instituto Nacional de Pesquisas da Amazônia (INPA), Coordenação de Pós-Graduação (COPOG), Divisão do Curso em Entomologia (DiEnt), Coordenação de Biodiversidade (CoBio), Manaus, Amazonas, Brazil. anampes@gmail.com Author Barcelos-Silva, Patrik BFE5C5B5-97A2-4CBB-8133-9737579F12D2 Instituto Nacional de Pesquisas da Amazônia (INPA), Coordenação de Pós-Graduação (COPOG), Divisão do Curso em Entomologia (DiEnt), Coordenação de Biodiversidade (CoBio), Manaus, Amazonas, Brazil. patrikbarcelos@gmail.com Author Hamada, Neusa 0D02F36C-6B09-423C-95A6-6179E9808CD5 Instituto Nacional de Pesquisas da Amazônia (INPA), Coordenação de Pós-Graduação (COPOG), Divisão do Curso em Entomologia (DiEnt), Coordenação de Biodiversidade (CoBio), Manaus, Amazonas, Brazil. neusaha@gmail.com text European Journal of Taxonomy 2020 2020-07-01 677 1 11 journal article 10.5852/ejt.2020.677 0e78be95-ea62-4470-8f90-d17ce4ffd47e 2118-9773 3928391 21205634-5160-48F3-B605-649AD771C58C Triplectides nessimiani Desidério & Pes sp. nov. urn:lsid:zoobank.org:act: 0B5D5E41-9729-47F5-8108-8 CAD 58114588 Figs 1–3 Diagnosis The male of Triplectides nessimiani sp. nov. is similar to that of T . ultimus by fork I in the hindwing having a very short petiole and both having a short, subtriangular mesal lobe on the inferior appendage. However, in T . nessimiani sp. nov. the mesal lobe has a slightly acute lateral projection and 5–7 stout ventral setae in the middle region, whereas T . ultimus has bifid lateral projection and three stout ventral setae. Furthermore, the new species can easily be distinguished by the preanal appendages, which are digitate with apices rounded (slender with apices pointed in T . ultimus ), tergum X with apex obliquely truncated (rounded in T . ultimus ) and phallotremal sclerite well developed, subpentagonal-shaped in dorsal view (simple in T . ultimus ). Etymology The new species is named in honor of Prof. Dr. Jorge Luiz Nessimian (Universidade Federal do Rio de Janeiro , Brazil ) who helped collect the type specimens and in recognition of his contributions to the study of the Neotropical caddisflies. Material examined Holotype BRAZIL • ♂ ; Amazonas , Barcelos , Parque Estadual Serra do Aracá , Igarapé da Anta (#S05); 00°54′38.70″ N , 63°25′54.70″ W ; alt. 1130 m ; 26 Jul.–2 Aug. 2009 ; N. Ferreira Jr , J.L. Nessimain , F.F. Salles , A.P.M. dos Santos , U.G. Neiss and J.O. da Silva leg.; Malaise trap ; INPA-TRI 000014 . Paratype BRAZIL • 1 ♂ ; Amazonas , Barcelos , Parque Estadual Serra do Aracá , Igarapé de 1º ordem (#S03); 00°54′22.03″ N , 63°27′33.23″ W ; alt. 1110 m ; 23 Jul.–1 Aug. 2009 ; J.O. Silva , J.L. Nessimian and U.G. Neiss leg.; suspended trap ; INPA-TRI 000015 . Fig. 1. Triplectides nessimiani Desidério & Pes sp. nov. , ♂ holotype (INPA-TRI 000014). A . Dorsal habitus. B . Venation of the forewing. C . Venation of the hind wing. Scale bars = 2 mm. Fig. 2. Triplectides nessimiani Desidério & Pes sp. nov. , ♂ genitalia, holotype (INPA-TRI 000014). A . Lateral view. B . Dorsal view. C . Ventral view. D . Phallic apparatus, lateral view. E . Phallotremal sclerite, dorsal view. Scale bars = 0.2 mm. Description Adult male ( Figs 1 A–C, 2A–E) LENGTH OF FOREWING. 8.12–8.22 mm (n = 2). Fig. 3. Distribution of Triplectides species treated in this study and general view of the streams where Triplectides nessimiani Desidério & Pes sp. nov. was collected. A . Distribution map of T . neblinus , T . nessimiani Desidério & Pes sp. nov. and T . nevadus . B . Igarapé da Anta, Amazonas, Brazil, stream where T . nessimiani Desidério & Pes sp. nov. holotype was collected. C . First order stream, Amazonas, Brazil, collection site of T . nessimiani Desidério & Pes sp. nov. paratype. COLOR. General color brown (in alcohol). Antennae with scape and pedicel golden-brown, flagellum pale-yellow ( Fig. 1A ) and palps brown. Forewing pale-brown ( Fig. 1A ), with small pale spots; with forks I and V present; discoidal cell about as broad as apical cells, nearly parallel-sided distally; cross vein s long, curved; crossvein r -m curved ( Fig. 1B ). Hind wing with forks I, III and V present; fork I with very short petiole ( Fig. 1C ). Legs pale-brown. Tibial spur formula 2:2:4. MALE GENITALIA ( Fig. 2 A–E). Segment IX, in lateral view, narrow, annular, with tergum IX produced posteriorly, anterior margin nearly straight, posterior margin produced medially ( Fig. 2A ); tergum IX with sclerotized posterior margin sharply rounded to slightly angulate and with small mesal notch ( Fig. 2B ), bearing rounded dorsomesal membranous process (absent in male paratype ). Preanal appendages setose, about ¾ length of tergum X; in dorsal view, digitate, apices rounded ( Fig. 2B ). Tergum X, in lateral view, with basal half less sclerotized than apical half, tall at base, narrowing apically ( Fig. 2A ), with ventral margin straight, apex rounded; in dorsal view, bearing short apical and lateral setae; apex obliquely truncated, with deep, V-shaped apicomesal incision extending anteriorly one-half the length of the segment ( Fig. 2B ). Inferior appendages long, surpassing tergum X, bearing long setae ( Fig. 2A ); each with 1 st article, in lateral view, enlarged basally, slightly constricted at mid-length ( Fig. 2A ); apicodorsal lobe digitate, long, extending beyond second article, with long setae ( Fig. 2A, C ); basoventral lobe digitate, long, apex rounded, bearing long setae ( Fig. 2A, C ); mesal lobe, in ventral view, shorter than basoventral lobe, subtriangular, bearing a row of 5–7 stout ventral setae basally, with two longitudinal striae, divided into long, blunt apical projection and slightly acute lateral projection, apex concave ( Fig. 2C ); 2 nd article short, slender, with pointed apex, directed apicomesad ( Fig. 2C ). Phallic apparatus elongate, complex; in lateral view, basal portion of phallobase subtriangular with ventral trough, middle portion curved and slender, apex with pair of wide, semimembranous flanges directed dorsolaterad; phallic membranes non-prominent, striate ( Fig. 2D ); phallotremal sclerite well developed, subpentagonal-shaped in dorsal view, with acute, thin median tip ( Fig. 2E ). Female and immature stages Unknown. Bionomics The males of T . nessimiani sp. nov. were collected in 1 st and 3 rd order streams with altitudes ranging from 1110 to 1130 m a.s.l. located in the Serra do Aracá mountain range, northern part of the Brazilian Amazon ( Fig. 3A ). The predominant vegetation is highland savanna ( Fig. 3B ), the water in the streams was black and acidic (pH 4.3–4.5) with low conductivity (10–20 µScm-1). At the sites where specimens were collected water temperatures of 21–22ºC were recorded and the streams were 1–5 m wide, 0.30–3 m deep, with rocky bottoms characterized by boulders and slow-flowing water ( Fig. 3 B– C). Distribution ( Fig. 3A ) Brazil (Amazonas state).