Two new species of the whip-spider genus Heterophrynus (Arachnida: Amblypygi) with complementary information of four species Author Chirivi-Joya, Daniel Laboratório de Entomología, Departamento de Biología, Facultad de Ciencias, Pontificia Universidad Javeriana. Carrera 7 N °. 40 - 62, Bogotá. & dhipnosodin @ hotmail. com; https: // orcid. org / 0000 - 0002 - 1198 - 782 X Author Moreno-González, Jairo A. Departamento de Zoologia, Instituto de Biociências, Universidade de São Paulo. Rua do Matão, trav. 14, n ° 321, Cidade Universitária, São Paulo- SP, CEP: 05508 - 090. hansenochrus @ gmail. com; https: // orcid. org / 0000 - 0001 - 5980 - 1417 Author Fagua, Giovanny Laboratório de Entomología, Departamento de Biología, Facultad de Ciencias, Pontificia Universidad Javeriana. Carrera 7 N °. 40 - 62, Bogotá. & fagua @ javeriana. edu. co; https: // orcid. org / 0000 - 0003 - 1253 - 4416 text Zootaxa 2020 2020-06-25 4803 1 1 41 journal article 10.11646/zootaxa.4803.1.1 1175-5326 3907900 2D391AAF-F105-4252-A090-34E725A05315 Heterophrynus armiger Pocock Figures 4–7 , 22 (in part). Table 2 Heterophrynus armiger Pocock, 1902: 162–163 ; Pocock 1903: 220 ; Mello-Leitão 1931: 47 ; Weygoldt 1972 : fig. 10; Weygoldt 1974 : figs 1, 4; Giupponi 2002: 173 ; Weygoldt 2002: 298 , fig 16; Víquez et al. 2014: 457–459 , figs: 1–5; Armas et al. , 2015: 69 ; Álvarez et al. , 2015: 45 ; Miranda , Kury & Giupponi 2018 : table 1; Vásquez et al. 2019: 33 . Type locality: Pambilar , Ecuador . Revised material ( 2 females , 7 males ): Colombia : Cauca : Guapi municipality, Natural National Park (N.N.P.) Gorgona , Isla Gorgona ( 2°57′53″ N , 78°10′30″ W ): Village 5m , at 13 masl , inside a house, 5 March 1990 , M. L. Baena , male (IAvH without number); in a house, April 23 of 1991, J. Portilla , manual collection, male (IAvH-I 2923); between bricks and construction material, 20 October 1991 , E. Solis , manual collection, 2 males (IAvH-I 2925, IAvH-I 2924). Sendero Cerro Los Micos ( 2°58´20,5´´ N , 78° 10´38,8´´ W ): 21 October 2010 , Zoology team, female (MUSENUV 24303). Playa Palmeras ( 2°56´28,6´´ N , 78° 12´21,4´´ W ): Forest behind of a house, 28 masl , 24 February 2011 , 1 male , 1 male juvenile (MUSENUV 24304). El Poblado ( 2°57´15,8´´ N , 78°10´6,6´´ W ): 2 December 1987 , Grupo de Insectos , male (MUSENUV 24307); September 1989 , M. Baena , female (MUSENUV 24306) . Distribution. Countries: Colombia , Ecuador . Other records from: Víquez et al. (2014) : Colombia , Gorgona; Ecuador , Pambilar, Durango River. Diagnosis. Heterophrynus armiger can be differentiated from other species by present the following characters together: four teeth in the ectal portion of the basal segment of the chelicerae. Five long spines in the ventral femur, spine FIII is smaller than FII and FV, FIV is absent or like as a tubercle. Female genitalia with gonopod sclerites elongated and with laminar shape, and an acuminated apex. Seminal receptacles oval with a sclerotized area at the base ( Fig. 6D ). Similar species: H. armiger is similar to H. alces and H. awa sp. nov. , however, H. alces presents a small spine TIII and T3, which are absent in H. armiger . The female genital is completely different from the one of H. alces , which presents the sclerites of the gonopods with a rounded shape, and H. awa sp. nov. , which have sclerites of the gonopods wider at the middle region. The male genital of H. armiger presents a spine in LOL 1, which is not present in any other species ( Fig. 7 ). Complementary description. Male (IAvH-I 2924): Total length 40.2mm . Carapace, opisthosoma, and legs have a black coloration, with brown areas, pedipalps black. Measures of the male are provided in Table 2 . Carapace. Anterior margin straight without lobes, carapace surface with a widely spaced granulation, posterior margin of carapace with two lobes. Lateral and anteromedial eyes clearly visible, anteromedial ocular tubercle with black coloration ( Fig. 4A ). Sternum. Tri-segmented, segments are well sclerotized; the area around segments is also sclerotized. Tritosternum projected anteriorly, elongated, conical, with 10 setae in the basal region, four in the medial region two in distal region and two more in the apex. The second segment (tetrasternum) rounded and with four setae; two in the basal region and two in the apical region. The third segment (pentasternum) rounded, smaller than the second segment, has four setae; one on each side of the apical region, one on each side of the basal region. Metasternum longitudinally divided with two setae in each segment ( Fig. 4B ). Abdomen. Oblong, color black and brown, with soft punctuations. Carapace wider than the Abdomen ( Fig. 4C ). FIGURE 4. Heterophrynus armiger . Male (IAvH-I 2924): A. Carapace. B. Sternum. C. Dorsal opisthosoma. D. Chelicera, mesal view. E. Trochanter (Tr). F. Chelicera, ectal view. Chelicera. Presents a mesal row of three teeth on the basal segment of the chelicerae with the first in the proximal region, with two cuspids, follow by one tooth shorter in the medial portion, and the third bigger than others placed in the distal region ( Fig. 4D ). The ectal row of the basal segment of the chelicerae have four conspicuous teeth, two with acuminated apex placed in the proximal and medial portions (tooth 1 and 2), and two with acuminated apex placed on the medial portion (tooth 3 and 4) ( Fig. 4F ). Mobile finger of the chelicerae with five teeth. FIGURE 5. Heterophrynus armiger . Male (IAvH-I 2924): A. Ventral femur (Fv). B. Dorsal femur (Fd). C. Ventral patella (Pv) . D. Dorsal patella (Pd) . E. Ventral tibia (Tv). F. Dorsal tibia (Td). Pedipalp. Trochanter : Prolateral face with four spines; spines Tr1 and Tr3 placed in the medial region, Tr2 placed near to ventral margin. Tr3 and Tr4 with subequal lengths. Spines lengths: Tr2>Tr1>Tr4≥Tr3. Dorsal oblique series of six setal tubercles. The dorsomedial area without spines but with one tubercle ( Fig. 4E ). Femur : Ventral face with five major spines, there are three tubercles between FII–FIII, and FIII–FV, a small spine and 12 tubercles, between FV–FVI, ten tubercles more distally than FVI, FIV absent. Spine lengths: FI>FII>FV>FVI>FIII ( Fig. 5A ). Dorsal face with five major spines, there are two tubercles between F2–F3, a very small spine with two tubercles at each side between F3–F4, a very small spine with three tubercles more proximal and two more distally between F4–F5, and more than ten tubercles more distally than F5. F1 and F2 are joined at the base, F3 clearly separated from F2. Spine lengths: F3>F4>F1=F2>F5 ( Fig. 5B ). Patella : Ventral face with five major spines, there are three tubercles between PV–PIV, three between PIV–PIII, four between PIII–PII and between PII–PI, PI wider than the other spines, PVI is absent. Spine lengths: PIV>PII>PV>PI>PIII ( Fig. 5C ). Dorsal face with five major spines, seven tubercles between P6–P5; P7, and P2 are absent. Spine lengths: P3=P4≥P5>P6>P1 ( Fig. 5D ). Tibia : Ventral face with two major spines. Spine lengths: TII>TI ( Fig. 5E ). Dorsal face with two major spines, with one tubercle between T2–T3. Spine lengths: T2>T1 ( Fig. 5F ). Tarsus-metatarsus: Internal face with the secondary line of dorsomedial bristles complete, the suture between the tarsus and the apotele is not visible. Legs . Femora brown. Femora lengths: I>III>II>IV ( Table 2 ). Leg I: Tibia with 31 segments and tarsus with 70 segments in the right and left leg. Leg IV: Basitibia with three segments. FIGURE 6. Male genital: Heterophrynus armiger . (IAvH-I 2924): A. ventral view. B. Detail of ornamentation of LoL 1 and LoL 2. C. Dorsal view. Fi = fistula ; LaM = lamina medialis ; LoD = lobus dorsalis ; LoL 1= lobus lateralis primus ; LoL 2 = lobus lateralis secundus ; Pi = processus internus of gonopod. D. Female genital (MUSENUV 24303), black arrow indicates the sclerotized area of the seminal receptacle. Basitibia-distitibia lengths: BT1>DT>BT3>BT2. Basitarsus shorter than telotarsus. Tarsus tetramerous. Female genitalia. (MUSENUV 24303): Genital operculum pentagonal, reaching the second segment of the opisthosoma, seminal receptacles with an oval shape, completely separated at the medial portion, dorsal surface sclerotized at the proximal region, with brown coloration, medial and distal portion white. Sclerites with laminar shape, distal portion a little curved and thinner than the basal portion, with acuminated apex, sclerites placed parallel, longer than the width of the seminal receptacle, sclerites cover almost the total length of the seminal receptacle ( Fig. 6D ). Variation. Variation in measures and segments of the basitibia IV, tibia I and tarsus I, are provided in Table 2 . Sternum . The number of setae over all segments of the sternum was variable. Pedipalp . Trochanter : line of setal tubercles vary between five and six. Femur : Ventral face: number of tubercles between spines are variable, the small spine between FV and FVI, and tubercles can be more conspicuous. In the specimen MUSENUV 24306, there is an FIV with the same size of FIII. Dorsal face: tubercles between spines are variable, can be present a very small spine between F4 and F5. Patella . Ventral face: number and size of the tubercles are variable, PVI can be present as a small spine. Dorsal face: number of tubercles are variable, P2 can be present. Tibia. Ventral and Dorsal face: number of tubercles between spines are variable. Legs. leg I: Number of segments of tibia vary between 31 and 32 and tarsus between 66 and 71 ( Table 2 ). Male. Observed Males were bigger than females ( Table 2 ); Spination pattern same as females. Genital operculum has an oval posterior margin, expanded to the third segment of the opisthosoma. Male genitalia . (IAvH-I 2924): LaM longer than Pi. LoL 1 longer than LoL 2, LoL 1 is wider, densely covered with minute conical projections, placed close together with the base not visible; LoL 2 is wrinkled and does not present projections. In both LoL 1, there is present a spine sticking out from the projections. In dorsal view, the LoD are elongated and do not cover LoL1 and LoL 2 ( Fig. 6A, B, C ; 7 A-B).