Two new species of the whip-spider genus Heterophrynus (Arachnida: Amblypygi) with complementary information of four species Author Chirivi-Joya, Daniel Laboratório de Entomología, Departamento de Biología, Facultad de Ciencias, Pontificia Universidad Javeriana. Carrera 7 N °. 40 - 62, Bogotá. & dhipnosodin @ hotmail. com; https: // orcid. org / 0000 - 0002 - 1198 - 782 X Author Moreno-González, Jairo A. Departamento de Zoologia, Instituto de Biociências, Universidade de São Paulo. Rua do Matão, trav. 14, n ° 321, Cidade Universitária, São Paulo- SP, CEP: 05508 - 090. hansenochrus @ gmail. com; https: // orcid. org / 0000 - 0001 - 5980 - 1417 Author Fagua, Giovanny Laboratório de Entomología, Departamento de Biología, Facultad de Ciencias, Pontificia Universidad Javeriana. Carrera 7 N °. 40 - 62, Bogotá. & fagua @ javeriana. edu. co; https: // orcid. org / 0000 - 0003 - 1253 - 4416 text Zootaxa 2020 2020-06-25 4803 1 1 41 journal article 10.11646/zootaxa.4803.1.1 1175-5326 3907900 2D391AAF-F105-4252-A090-34E725A05315 Heterophrynus alces Pocock Figures 1–3 , 22 (in part). Table 1 Heterophrynus alces Pocock, 1902: 163–164 ; Mello-Leitão 1931: 47 ; Weygoldt 1972 : fig. 10; Weygoldt 1974 : fig. 5; Weygoldt 1977: 275–276 , figs 2a-c, 3b; Weygoldt 1977: 363–369 , fig. 1; Hammen 1986: 4 , figs 2c-d, 4c,7e-f, 11b-c, 19a-e; Quintero 1986: 211 ; Hammen 1989: 484 , figs 3c-d, 256c, 257e-f, 259b-c, 262a-e; Weygoldt 1994 : fig. 2a; Weygoldt 2002: 298 , fig 14; Giupponi 2002: 173 ; Giupponi & Kury, 2013: 330 ; Lehmann & Friedrich 2018: 171 , table 1, 3. Admetus alces (Pocock) : Caporiacco 1948: 615 . Type locality: Upper Suriname River, Suriname . Examined material ( 22 females , 25 males , 5 juveniles ): BRAZIL : Amapá : Oiapoque : Taparabó ( 4°00´50.3´´ N ; 51°41´22.8´´W ): Rain forest , over a wide trunk, 31 March 2015 , D. Chirivi , J. Murienne , manual collection at night, 1 male , 1 female , 1 juvenile ( MZUSP 74497 ); Rain forest , over a wide trunk, 31 March 2015 , D. Chirivi , J . Murienne , manual collection at night, male ( MZUSP 74496 ) . Tumucumaque ( 3°16´58.9´´ N ; 52°12´06.5´´W ): in front of Carbet Mitemps , Rain forest , over a wide trunk, 23 March 2015 , D. Chirivi , J. Murienne , manual collection at night, 2 males , 1 female ( MZUSP 74502 ) ; in front of Carbet Mitemps , Rain forest , over a wide trunk, 23 March 2015 , D. Chirivi , J. Murienne , manual collection at night, 2 male , 1 female ( MZUSP 74505 ) ; in front of Carbet Mitemps , Rain forest , over a wide trunk, 23 March 2015 , D. Chirivi , J. Murienne , manual collection at night, 2 juveniles ( MZUSP 74506 ) ; in front of Carbet Mitemps , Rain forest , over a wide trunk, 18 March 2015 , D. Chirivi , J. Murienne , manual collection at night, male ( MZUSP 74508 ) ; in front of Carbet Mitemps , Rain forest , over a wide trunk, 22 March 2015 , D. Chirivi , J. Murienne , manual collection at night, 2 males , 1 female , 1 juvenile ( MZUSP 74509 ) ; in front of Saut Maripa , Rain forest , over a wide trunk, 17 March 2015 , D. Chirivi , J. Murienne , manual collection at night, 2 males ( MZUSP 74510 ) ; in front of Saut Maripa , Rain forest , over a wide trunk, 17 March 2015 , D. Chirivi , J. Murienne , Manual collection at night, 3 females ( MZUSP 74516 ) ; in front of Saut Maripa , Rain forest , over a wide trunk, 18 March 2015 , D. Chirivi , J. Murienne , manual collection at night, 2 males ( MZUSP 74517 ) . Pará : Almeirim : Estação Jari (0°35´91.1´´S; 52°41´72.4´´W): 12–19 June 2004 , 1 female , 2 males ( MPEG 00172 ) ; 22 June 2005 , T . Gardner, A . Ribeiro , female ( MPEG 00171 ) . FRENCH GUIANA : Carbet Mitemps : Oiapoque River ( 3°17´50,59´´N , 52°10´22,01´´W ): Rain forest , over a wide trunk, 20 March 2015 , D. Chirivi , J. Murienne , manual collection at night, 1 male , 1 female ( MZUSP 74500 ) ; Rain forest , over a wide trunk, 25 March 2015 , D. Chirivi , J. Murienne , manual collection at night, 1 male , 2 females ( MZUSP 74501 ) ; Rain forest , over a wide trunk, 24 March 2015 , D. Chirivi , J. Murienne , manual collection at night, 1 male , 1 female ( MZUSP 74507 ) ; Rain forest , over a wide trunk, 24 March 2015 , D. Chirivi , J. Murienne , manual collection at night, 1 male , 3 females ( MZUSP 74511 ) ; Rain forest , over a wide trunk, 26 March 2015 , D. Chirivi , J. Murienne , manual collection at night, 1 female ( MZUSP 74512 ) ; Rain forest , over a wide trunk, 26 March 2015 , D. Chirivi , J. Murienne , manual collection at night, 2 males ( MZUSP 74514 ) . Saut Maripa : Oiapoque River ( 3°18´54.8´´N ; 52°11´37.5´´W ): Rain forest , over a wide trunk, 14 March 2015 , D. Chirivi , J. Murienne , manual collection at night, female ( MZUSP 74499 ) ; Rain forest , over a wide trunk, 14 March 2015 , D. Chirivi , J. Murienne , manual collection at night, 2 females ( MZUSP 74498 ) ; Rain forest , over a wide trunk, 15 March 2015 , D. Chirivi , J. Murienne , manual collection at night, 2 males ( MZUSP 74503 ) ; Rain forest , over a wide trunk, 15 March 2015 , D. Chirivi , J. Murienne , manual collection at night, 1 female , 1 male ( MZUSP 74504 ) . Trois Palétuviers : Oiapoque River : Rain forest , over a wide trunk, 30 March 2015 , D. Chirivi , J. Murienne , manual collection at night, juvenile ( MZUSP 74513 ) ; Rain forest , over a wide trunk, 29 March 2015 , D. Chirivi , J. Murienne , manual collection at night, 1 male , 1 female ( MZUSP 74515 ) . Distribution: Countries: Brazil , French Guyana , Guyana , Surinam . Other records from: Weygoldt (2002) ; Weygoldt (1977) : Brasil , Amapá , Serra do Navío; Hammen (1986) : Surinam , Saramacca river. Diagnosis. Heterophrynus alces can be differenced from other species by presents the following characters together: three teeth in the ectal portion of the basal segment of the chelicerae. Six long spines in the ventral femur, spine FIII is smaller than FII and FV; it presents a small spine TIII and T3. Female genitalia with a rounded basal area and a distal portion curved. Seminal receptacles with a sclerotized area, which reach the apical part ( Fig. 3D ). The male genital of H. alces differs from other evaluated species by presenting a LoL 1 shorter than the LaM and the Pi. The LoL 2 is smooth and, in some regions, covered by minute projections, while, in the other species the LoL 2 is wrinkled and without projections ( Fig. 3A, B, C ). Similar species: No other species present all these characters together, the most similar are H. armiger and H. awa sp. nov. , the only character shared with these species is that all present the spine FIII smaller than FII and FV. Complementary description. Female (MZUSP 74499): Total length 23mm . Carapace, opisthosoma, and legs have a brown coloration, with yellow areas, pedipalps brown and red. Measures of the female are provided in Table 1 . Carapace. Anterior margin straight without lobes, carapace surface with a widely spaced granulation, posterior margin of carapace with a concave medial region. Lateral and anteromedial eyes clearly visible, anteromedial ocular tubercle with black coloration ( Fig. 1A ). Sternum. Tri-segmented, segments are well sclerotized; the area around segments is also sclerotized. Tritosternum projected anteriorly, elongated, conical, with 16 setae in the basal region, two in the medial region, five in the distal region and two more in the apex. The second segment (tetrasternum) rounded and with six setae; four in the basal region and two in the apical region. The third segment (pentasternum) rounded, smaller than the second segment, has four setae; one on each side of the apical region, and one on each side of the basal region. Metasternum longitudinally divided with two setae in each segment ( Fig. 1B ). Abdomen. Oblong, color yellow and brown, with soft punctuations. Presents patches of light coloration at both sides of each tergite. Carapace wider than the Abdomen ( Fig. 1C ). FIGURE 1. Heterophrynus alces . Female (MZUSP 74499): A. Carapace. B. Sternum. C. Dorsal opisthosoma. D. Chelicera, mesal view. E. Trochanter (Tr). F. Chelicera, ectal view. TABLE 1. Measurements (mm) of the body, pedipalp segments, and femora of the legs in six specimens of Heterophrynus alces . Empty cells correspond to missing segment or data.

MZUSP 74499		MZUSP 74508		MZUSP 74501		MZUSP 74501		MPEG 00172		MPEG 00172
Sex	Female	Male	Female	Female	Female	Male
Total length
(with/without chelicerae)	26.2/24.4	30.7/28.3	27.8/26	25.5/23.7	18.4/17.3	24.1/22
Prosoma
Maximum width	14.8	15.6	13.5	12.7	10.1	11.8
Maximum length	11.6	12.2	10.3	9.7	7.0	8.4
Opisthosoma
Maximum width	9	11.6	10.5	9	8.1	9.9
Maximum length	15.7	20	18.3	16.4	10.9	16.2
Right	Left	Right	Left	Right	Left	Right	Left	Right	Left	Right	Left
Pedipalp lengths
Trochanter	5	5	5.4	5.4	4	4	4.1	4.1	2.9	2.9	3.8	3.8
Femur	11.7	11.7	13.6	13.6	10.5	10.5	9.2	9.2	6.5	6.1	9.6	9.5
Patella	12.6	12.5	14.6	14.6	11.7	11.2	10	10	8.3	8.4	10	10
Tibia	7	7	6.9	7.3	6.3	6.3	4.9	4.9	3.5	3.4	6.2	6.2
Tarsus-metatarsus	5.4	5.4	5.8	5.8	4.9	4.9	4.4	4.1	2.9	3.0	4.2	4.2
Femur length
Leg I	28.5	29.3	33.7	30.5	25.8	25.8	23	21.1	17.1	16.4	24.1	23.9
Leg II	21.9	21.6	25.3	25.4	19.6	19.4	17.7	17.8	12.2	11.3	18.4	18.1
Leg III	21.8	21.8	25.2	24.4	19.6	19.6	17.9	17.7	10.9	11.0	18	18.4
Leg IV	19.7	19.3	21.8	21.7	17.8	17.8	16.2	15.7	11.1	11.3	16.3	16.4
No. segments tibia I	31	31	31	47	31	31	31	31	31
No. segments tarsus I	68	68	72	80	71	72	64	71	68
No. segments basitibia IV	3	3	3	3	3	3	3	3	3	3	3	3

Chelicera. Presents a mesal row of three teeth on the basal segment of the chelicerae, with the first in the proximal region, with two cuspids follow by one tooth shorter in the medial portion, and the third bigger than others placed in the distal region ( Fig. 1D ). The ectal row of the basal segment of the chelicerae have three conspicuous teeth, two with acuminated apex placed in the proximal and medial portions (tooth 1 and 2), and one with rounded apex placed on the medial portion (tooth 3) ( Fig. 1F ). Mobile finger of the chelicerae with five teeth. FIGURE 2. Heterophrynus alces . Female (MZUSP 74499): A. Ventral femur (Fv). B. Dorsal femur (Fd). C. Ventral patella (Pv) . D. Dorsal patella (Pd) . E. Ventral tibia (Tv). F. Dorsal tibia (Td). Pedipalp. Trochanter : Prolateral face with four spines; spines Tr1 and Tr3 placed in the medial region, Tr2 placed near to ventral margin. Tr3 and Tr4 with subequal lengths. Spines lengths: Tr2>Tr1>Tr3≥Tr4. Dorsal oblique series of six setal tubercles. The dorsomedial area without spines but with one tubercle ( Fig. 1E ). Femur : Ventral face with six major spines, there are two tubercles between FII–FIII, and FIII–FV, a small spine between FV–FVI, with two tubercles at each side, a small spine between FVI–FVII and three tubercles more distally than FVII. Spine lengths: FI>FII>FV>FVI>FIII>FVII ( Fig. 2A ). Dorsal face with five major spines, there are two tubercles between F3–F4, five between F4–F5, and five more distally than F5, F1 and F2 are joined at the base, F3 clearly separated from F2. Spine lengths: F3>F4>F1=F2>F5 ( Fig. 2B ). Patella : Ventral face with five major spines, there are six tubercles between PV–PIV, one between PIV–PIII, two between PIII–PII and between PII–PI, PI wider than the other spines, PVI absent. Spine lengths: PIV>PV>PII>PI>PIII ( Fig. 2C ). Dorsal face with six major spines, two tubercles between P6–P5, P7 is absent. Spine lengths: P3>P4=P5>P1>P2>P6 ( Fig. 2D ). Tibia : Ventral face with two major spines, and one very small spine TIII, with one tubercle between TII–TIII. Spine lengths: TII>TI>TIII ( Fig. 2E ). Dorsal face with two major spines, and one very small spine T3, with one tubercle between T2–T3. Spine lengths: T2>T1>T3 ( Fig. 2F ). Tarsus-metatarsus: Internal face with the secondary line of dorsomedial bristles complete, the suture between the tarsus and the apotele is not visible. Legs . Femora brown. Femora lengths: I>II>III>IV ( Table 1 ). Leg I: Tibia with 31 segments and tarsus with 68 segments in the right and left legs. Leg IV: Basitibia with three segments. Basitibia-distitibia lengths: BT1>DT>BT3>BT2. Basitarsus shorter than telotarsus. Tarsus tetramerous. FIGURE 3. Male genital: Heterophrynus alces . (MZUSP 74504): A. ventral view. B. Detail of ornamentation of LoL 1 and LoL 2. C. Dorsal view. Fi = fistula ; LaM = lamina medialis ; LoD = lobus dorsalis ; LoL 1= lobus lateralis primus; LoL 2 = lobus lateralis secundus; Pi = processus internus of gonopod. D. Female genital (MZUSP 74499), black arrow indicates the sclerotized area of the seminal receptacle. Female genitalia. Genital operculum pentagonal, reaching the second segment of opisthosoma, seminal receptacles with a rounded shape, completely separated at the medial portion, dorsal surface sclerotized at the proximal and medial region reaching a portion of the distal region, with orange coloration; distal portion white. Sclerites with a rounded base, medial and distal portion curved and thinner than the basal portion, with rounded apex; sclerites placed parallel, shorter than the width of the seminal receptacle, sclerites cover just a half of the total length of the seminal receptacle ( Fig. 3D ). Variation. Variation in measures and segments of the basitibia IV, tibia I and tarsus I, are provided in Table 1 . Sternum . The number of setae over all segments of the sternum was variable. Pedipalp . Trochanter : line of setal tubercles vary between four and eight. Patella . Ventral face: can be present small spines at each side of PV. Dorsal face: can be present small spines at each side of P6. Male. Observed Males have a similar size with the females ( Table 1 ), Spination pattern same as females. Genital operculum has an oval posterior margin, expanded to the third segment of the opisthosoma. Male genitalia . (MZUSP 74504) : LaM as long as Pi. LoL 1 longer than LoL 2, LoL 1 is wider, densely covered with minute projections, placed close together with the base not visible; LoL 2 smooth and with few projections, the base is visible. In dorsal view, the LoD are rounded covering LoL1 and LoL 2 ( Fig. 3A, B, C ).