Phyllactinia himalayensis, a new species on Juglans regia from Pakistan Author Zafar, Irsa 0000-0003-1173-5064 Institute of Botany, University of the Punjab, Quaid-e-Azam Campus- 54590, Lahore, Pakistan. & irsazafar 2 @ gmail. com; https: // orcid. org / 0000 - 0003 - 1173 - 5064 irsazafar2@gmail.com Author Afshan, Najam-Ul-Sehar 0000-0003-4538-3626 Institute of Botany, University of the Punjab, Quaid-e-Azam Campus- 54590, Lahore, Pakistan. & pakrust @ gmail. com; https: // orcid. org / 0000 - 0003 - 4538 - 3626 pakrust@gmail.com Author Khalid, Abdul Nasir 0000-0002-5635-8031 Institute of Botany, University of the Punjab, Quaid-e-Azam Campus- 54590, Lahore, Pakistan. & drankhalid @ gmail. com; https: // orcid. org / 0000 - 0002 - 5635 - 8031 drankhalid@gmail.com Author Liu, Shu-Yan 0000-0001-9082-1053 College of Plant Protection, Jilin Agricultural University, Changchun 130118, Jilin Province, People’s Republic of China. & liussyan @ 163. com; https: // orcid. org / 0000 - 0001 - 9082 - 1053 Author Tang, Shu-Rong 0000-0002-1071-0335 College of Plant Protection, Jilin Agricultural University, Changchun 130118, Jilin Province, People’s Republic of China. & 761764101 @ qq. com; https: // orcid. org / 0000 - 0002 - 1071 - 0335 761764101@qq.com text Phytotaxa 2022 2022-05-31 548 2 267 276 journal article 61141 10.11646/phytotaxa.548.2.9 7ad914fb-55d1-4ea5-8ec1-f359762a4d40 1179-3163 6597880 Phyllactinia himalayensis I. Zafar, Afshan & Khalid sp. nov . ( Figs 2 , 3 ) MycoBank no. 843800 Diagnosis:— Phyllactinia himalayensis is distinguished from the phylogenetically allied species Ph. betulae and Ph. linderae by its large-sized chasmothecia, up to 280 µm diam., rather shorter appendages, 68‒184 µm, small ascospores, 10‒20 × 7‒13 µm, and its distinct position in the phylogenetic analysis. Type:— PAKISTAN . Azad Jammu & Kashmir : Poonch, 33.76°N , 74.09°E , 1,006 m alt., on leaves of Juglans regia L ., 9 Dec. 2021 , I . Zafar and N . S . Afshan [AJK-07] ( LAH 37119– holotype ). GenBank accession number: ( ON 332255 ) . Etymology:— the species epithet refers to the type locality, Himalayan forest, Pakistan . Description:— MYCELIUM hypophyllous, external, effuse, evanescent.CHASMOTHECIA scattered to gregarious, black, globose to subglobose, 190‒280 µm diam. PERIDIUM CELLS conspicuous, regularly hexagonal, sometimes polygonal, 7‒20 µm diam. APPENDAGES equatorial, 6–12, stiff, 68‒184 µm long and 5‒12 µm wide, bulbous basal swelling 21‒27 × 33‒40 µm, apex pointed, 0.5‒1 times as long as the chasmothecial diam., thick-walled, up to 6 µm wide below. ASCI 5‒10, broad ellipsoid, obovoid, clavate, 55‒86 × 20‒38 µm, short stalked, content yellowish, wall up to 3 µm thick, 2‒3-spored. ASCOSPORES ellipsoid to ovoid, 10‒20 × 7‒13 µm, colourless, sometimes yellowish. Additional material examined:— PAKISTAN . Azad Jammu & Kashmir : District Bagh, 33.9735°N , 73.7918°E , 1676 m alt., on leaves of Juglans regia , 9 Oct. 2020 , I . Zafar and N.S. Afshan [AJK-30] ( LAH 37118). GenBank accession number: ( ON 332222 ) .