Review of subfamily Prodiamesinae (Diptera: Chironomidae) from the Russian Far East and bordering territory Author Makarchenko, Eugenyi A. 0000-0003-2765-8729 Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far East Branch of the Russian Academy of Sciences, 100 let Vladivostoku 159, 690022 Vladivostok, Russia & makarchenko @ biosoil. ru; http: // orcid. org / 0000 - 0003 - 2765 - 8729 makarchenko@biosoil.ru Author Semenchenko, Alexander A. 0000-0001-7207-9529 Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far East Branch of the Russian Academy of Sciences, 100 let Vladivostoku 159, 690022 Vladivostok, Russia & semenchenko _ alexander @ mail. ru; https: // orcid. org / 0000 - 0001 - 7207 - 9529 semenchenko_alexander@mail.ru text Zootaxa 2023 2023-07-31 5323 1 1 26 http://dx.doi.org/10.11646/zootaxa.5323.1.1 journal article 10.11646/zootaxa.5323.1.1 1175-5326 8203829 AABE0C7B-9A90-4CF9-9707-936FF912853D Monodiamesa fontinalis Makarchenko et Semenchenko , sp. nov. http://zoobank.org/NomenclaturalActs/ 0F1A8DAF-C37A-46EB-9F0F-415F08589B7E ( Figs. 1–2, 4, 6 ) Type material. Holotype : adult male, Russia : Russian Far East , Jewish Autonomous Region , Obluchie District , Fedotkin Spring of Bidzhan River basin ( Amur River basin), 48.644139 N , 131.621685 E , 5.IV.2014 , leg. E. Makarchenko. Paratypes : 1 adult male, the same data as holotype ; 3 larvae of fourth instar, the same data as holotype, except Kolgaevskyi Spring, 48.645945 N , 131.622981 E , 8.IV.2014 , leg. E. Makarchenko. Derivatio nominis. The name of the new species comes from the Latin fontinalis , which means spring. Description Adult male (n = 1). Total length 6.4 mm . Total length/wing length 1.57. Coloration. Head, thorax, legs, and abdomen brown to dark brown; antennae light brown; wings greyish. Head. Eyes bare, with long parallel-sided dorsomedial extention. Temporal setae including 4 coronals, 8–14 preoculars, 14–22 verticals, and 7–11 postorbitals. Clypeus with 23–24 setae. Antenna with 13 flagellomeres and developed plume; terminal flagellomere without subapical setae; AR 2.0. Palpomeres lengths (in μm): 60; 100; 184; 180; 212. Palpomeres 1–5 length/head width 0.83. Thorax. Antepronotum with 5–6 lateral setae. Acrostichals absent, dorsocentrals 13, prealars 7, scutellars ca 60. Wing. Length 4.08 mm , width 0.92 mm . R and R 1 with 10; R 4+5 with 1 seta in distal part. Costa extension 115 μm long. RM length/MCu length 1.22–1.3. Anal lobe developed, outline rounded. Squama with 32 setae 105–150 μm long. VR 1.03. FIGURES 1–7. Adult males of Monodiamesa fontinalis sp. nov. (1–2, 4, 6) and M. bathyphila Kieffer (3, 5, 7). 1, 3, hypopygium in dorsal view; 2, hypopygium in ventral view; 4–7, median volsellae. Scale bar is 50 μm. Legs. Spur of fore tibia 96 μm long; spurs of mid tibia 72 μm and 60 μm; of hind tibia 96 μm and 72 μm long. Hind tibial comb with 12 setae. Lengths and proportions of leg segments as in Table 1 . Hypopygium ( Figs. 1–2, 4, 6 ). Tergite IX with 14–16 setae (from one side), 20–28 μm long and anal point 64–68 μm long, which with apical seta 20–24 μm long ( Fig. 1 ). Laterosternite IX with 9–14 setae. Gonostylus 168–176 μm long, covered with short setae, with megaseta 20 μm long and 1–2 setae nearest, 20–24 μm long ( Figs. 1–2 ). Gonocoxite 436–440 μm long, shape of superior and inferior volsellae as in Figs 1–2, 4, 6 . Length of superior volsella 84–96 μm, inferior volsella 88–100 μm. Median volsella 116–120 μm long, without pedestal for seta, in basal part with 3–4 setae ( Figs.4, 6 ). HR 2.5–2.6. TABLE 1. Lengths (in µm) and proportions of leg segments of Monodiamesa fontinalis sp. nov. (n=1).

fe	ti	ta1	ta2	ta3	ta4	ta 5	LR	BV	SV
P 1	1378	1673	1378	672	492	344	230	0.82	2.55	2.21
P2	1427	1574	771	459	361	279	197	0.49	2.91	3.89
P3	1480	1800	1080	640	492	295	230	0.60	2.63	3.04

Pupa unknown. Larva can be separated from M. bathyphila only by molecular data. Diagnosis. Male of M. fontinalis sp. nov. is very closely related to M. bathyphila from which it differs only in a few characters, namely, indexes of the antennae and legs (AR 2.0, LR 1 0.82), fewer dorsocentrals (13), longer Superior volsella (84–96 μm) and Median volsella (116–120 μm), Median volsella without pedestal for seta ( Table 2 ). In male M. bathyphila AR 2.76–3.34, LR 1 0.74–0.77, dorsocentrals 18–22, Superior volsella 40–60 μm long, Median volsella 60–112 μm long and with pedestal ( Figs. 5, 7 , Table 2 ). Distribution. Known only from type locality in Amur River basin and from South Korea (see below in part on DNA barcoding).