Phylogeny of the old and fragmented genus Austrocoenosia Malloch reveals new evidences on the morphology and evolution of the genera Coenosia Meigen and Neodexiopsis Malloch (Diptera: Muscidae) Author Patitucci, Luciano Damian https://orcid.org/0000-0002-9252-6071 Museo Argentino de Ciencias Naturales " Bernardino Rivadavia " (MACN), Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Buenos Aires, Argentina lpatitu@yahoo.com.ar Author Mulieri, Pablo Ricardo Museo Argentino de Ciencias Naturales " Bernardino Rivadavia " (MACN), Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Buenos Aires, Argentina Author Couri, Marcia Souto Museu Nacional, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil & Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), Brazil Author Dominguez, Martha Cecilia Laboratorio de Entomologia, Instituto Argentino de Investigaciones de Zonas Aridas (IADIZA-CONICET) Mendoza, Mendoza, Argentina text Arthropod Systematics & amp; Phylogeny 2023 2023-07-21 81 611 653 http://dx.doi.org/10.3897/asp.81.e104969 journal article http://dx.doi.org/10.3897/asp.81.e104969 1864-8312-81-611 8C3E7D063B2548429F28A76926D4A741 D6555CEAD4F355FF9A3D61D126ABBDE6 Coenosia delneneo sp. nov. Male (Fig. 8A). Length. Body: 5.56-5.90 mm, wing: 4.1-4.69 mm. Head : (Fig. 8B ). Frons at vertex about one third of the head width. Frons, fronto-orbital plate, parafacial and gena black with yellow-grey pollinosity. Fronto-orbital plate with 1-3 little setulae, close to parafacialia. Antenna dark brown, apex of pedicel with yellow pollinosity; arista brown with its longest microtrichia hardly longer than its basal diameter. Palpus black. Thorax : (Fig. 8C ). Grey with light brown pollinosity. Chaetotaxy: acr s strong and biseriate; anterior presutural acr s shorter than the eaDC; dorsocentrals 1+3, eaDC is half (or more) of length of the aDC. Anepisternum with a series of 4-5 strong setae; katepisternum with 2-3 setula. Wing : Both calypters whitish hyaline with white margins. Legs : Coxae grey. Fore femur yellow with a black large spot on dorsal to posterior surface. Trochanters, femora, tibiae, and 1-4 tarsomeres yellow. Fifth tarsomere black. Fore femur with a row of strong pd setae, and a row of strong pv setae. Mid femur with a row of strong setae on ventral surface, and 5-6 setae on anterior surface; mid tibia with one ad and one pd median setae with the same length, ad seta positioned below of the pd seta. Hind femur with a complete row of ad and a complete row of av setae, two setae on anterior surface at middle, and a row of setae on ventral surface, hind tibia with 3 preapical setae (ad, dorsal, ventral). Abdomen : (Fig. 8D ). Grey without spots, tergite 6 and epand brighter than the other tergites. Sternite 5 with apical margin concave ( "U" shape) and membrane; setae concentrated on the lobes and some long and strong on apical margin (Fig. 8E ). Terminalia : Cercus with strong spines on apical middle (Fig. 8F, G ). Figure 8. Coenosia delneneo sp. nov. , male. A Lateral view, B head, frontal view, C thorax, dorsal view, D abdomen, dorsal view, E fifth sternite, F cercus, G cercus and surstylus, lateral view, H detail of cercus, distal tip (SEM), I phallic complex, lateral view, J detail of phallic complex, distal tip. Scale bar: 1 mm (A), 0.5 mm (B-E), 0.2 mm (F, G), 0.02 mm (H), 0.01 mm (J, K). Female (Fig. 9A, B). Length. Body: 5.70-6.00 mm, wing: 4.8-5.12 mm. Differs from male as follows: Legs : Mid tibia with one ad and one pd median setae, both setae with the same length and position. Ovipositor : Segments longer than wide. Tergites 7 and 8 with two long and wide sclerotized plates (Fig. 9C ). Sternites 6 and 7 with one short and thin plates; sternite 8 divided into two small and sclerotized plates each with 4-5 setae on distal margin (Fig. 9D ). Three spermathecae, oval shaped, with a small prominence at the opposite end of the insertion of the tracheola (Fig. 9E, F ). Figure 9. Coenosia delneneo sp. nov. , female. A Lateral view, B head, frontal view, C ovipositor, dorsal view, D ovipositor, ventral view, E spermathecae, F details of spermathecae (SEM). Scale bar: 1 mm (A), 0.5 mm (B-D), 0.2 mm (E), 0.05 mm (F). Sixth and seventh sternites - red arrows, spermathecae small prominence - white arrow. Type material. Holotype . Male; MACN [pinned]. Original labels: "Arg. Neuquen , Alumine / Rio Alumine 896 m / 39°14 '26.03'' S 70°54 '43.93'' W / JAN2013 Olea, Mulieri & Patitucci leg." print. on white paper; "MACN-En / 34728" print. on white paper.; "Holotype" print. on red paper, black frame. - Paratypes . One male; MACN [pinned]. Original labels: "Arg. Neuquen , Alumine / Rio Alumine 896 m / 39°14 '26.03'' S 70°54 '43.93'' W / JAN2013 Olea, Mulieri & Patitucci leg." print. on white paper; "MACN-En / 34729" print. on white paper.; "Paratype" print. on red paper, black frame. Seven females; MACN [pinned]. Original labels: "Arg. Neuquen , Alumine / Rio Alumine 896 m / 39°14 '26.03'' S 70°54 '43.93'' W / JAN2013 Olea, Mulieri & Patitucci leg." print. on white paper; "MACN-En / 34721 [to] 37427" print. on white paper.; "Paratype" print. on red paper, black frame. Two males and one female; MLP [pinned]. Original labels: "Arg. Neuquen , PN Lanin / Huechulafquen 1070 m (transecta) / 39°47 '33.25'' S 71°12 '56.26'' W / 13-XII-2013 Mulieri leg." print. on white paper, "Paratype" print. on red paper, black frame. One male and 2 females; IFML [pinned]. Original labels: "Arg. Neuquen , Ruta 13 / Pampa de Lonco Luan / 1522 m a.s.l. transecta / -38.897919 -70.890376 / 09.II.2018 Patitucci leg." print. on white paper, "Paratype" print. on red paper, black frame. Additional material examined. ARGENTINA - Rio Negro province • 1 female , 1male ; El Bolson , Piltriquitron ; -41.972377 , -71.478712 ; 1190 m a.s.l. ; 12 Jan 2012 ; Mulieri & Patitucci leg.; MACN . - Neuquen province • 2 females ; Arroyo Guyapa , Ruta 13, -38.859349 , -70.472570 ; 1240 m a.s.l. ; 07 Feb 2018 ; Mulieri & Patitucci leg.; MACN • 1 male ; Primeros Pinos , Portezuelo La Atravezada ; -38.891693 , -70.65219 2; 1876 m a.s.l. ; 08 Feb 2018 ; Mulieri leg.; MACN • 3 females ; Ruta 23; -39.809776 , -71.030665 ; 16 Dec 2013 ; Mulieri & Patitucci leg.; MACN . Distribution (Fig. 12B). ARGENTINA: Neuquen , Rio Negro. Etymology. The name "delneneo" refers to an expression in Spanish to the vulgar name (neneo) of the plant Mulinum spinosum Pers., where several specimens were collected. Remarks. Coenosia delneneo sp. nov. is distinguished from its congeners by the following combination of characters: gena height greater than the width of the postpedicel, thorax and abdomen gray without vitta or spots, fore femur yellow with a black large spot on dorsal to posterior surface, fifth tarsomere black, dorsocentrals 1+3, with an extra short presutural pair which is half (or more) of the length of the presutural seta, hind tibia with one long ad seta, a long pd seta, and one fine av setae, and cercus longer than broad and with strong spines. Male: tergite 6 and epandrium visible and brighter, distiph with a brush of fine hair on ventral surface. Female: ovipositor in tergites 6 with two long and wide sclerotized plates and two square strongly sclerotized plates, and epiproct with "boomerang" shape. In Stein's key (1911), C. delneneo comes close to Coenosia pilitibia Stein 1911 , but differs in the chaetotaxy of the mid tibia. In Malloch' key (1934), C. delneneo comes close to C. ignobilis Stein, 1911, but the coloration of the fore femur and the shape of the abdomen can separate it from the latter.