Four new species of the subfamily Candoninae (Crustacea, Ostracoda) from freshwater habitats in Japan Author Smith, Robin James D63C5DD6-C0BB-43EA-8D2F-98134CC29188 Lake Biwa Museum, 1091 Oroshimo, Kusatsu, Shiga 525 - 0001, Japan. Email: smith @ lbm. go. jp (corresponding author) & urn: lsid: zoobank. org: author: D 63 C 5 DD 6 - C 0 BB- 43 EA- 8 D 2 F- 98134 CC 29188 Author Kamiya, Takahiro 5FB5FC58-0FBF-45DA-9FDC-7569BDA1FDF9 College of Science and Engineering, School of Natural System, University of Kanazawa, Kakuma, Kanazawa 920 - 1192, Japan. & urn: lsid: zoobank. org: author: 5 FB 5 FC 58 - 0 FBF- 45 DA- 9 FDC- 7569 BDA 1 FDF 9 text European Journal of Taxonomy 2015 2015-09-07 136 1 34 journal article 22395 10.5852/ejt.2015.136 2bc5e28e-c045-49d7-aaf2-5f5be028eb21 2118-9773 3785302 530F395F-97A9-46F1-957C-8E57B9C3ACD9 Pseudocandona becca sp. nov. urn:lsid:zoobank.org:act: F3A75D11-C834-4673-817D-E962EA9F9347 Figs 2 A–E, 3–5, 6A Pseudocandona sp. – Smith et al. 2014 : appendix S1. Diagnosis Posterior margin more inflated than anterior margin, hinge straight, slightly sloping towards anterior. Calcified inner lamella with distinctive convexity in antero-ventral region. Dorsal view with slight anterior beak. Surface of valves without pits. Second endopodal segment of male antenna sub-divided and with long male bristles (t2 and t3). Female antennal claw G2 approximately two-thirds length of G3. Mandible with 3+1+beta setae on second segment of palp, and with long gamma seta and short alpha and beta setae; beta slightly shorter than alpha. Walking leg with long d1 seta, and very short e, f and g setae. Seventh limb with five segments, and short, curved, reflexed h1 seta and long h2 and h3 setae. Outer and inner lobes (a and b respectively) of hemipenes sub-equal in length. Outer lobe (a) subquadrate distally, inner lobe (b) sub-triangular distally. Medial lobe (h) shorter than other lobes, with distinctive small, triangular to quadrate protrusion on outer edge. Etymology From the Latin beccus , meaning “beak” or “bill”, and referring to the small but characteristic, beakshaped projection on the medial lobe (h) of the hemipenes (marked with a black triangle on Fig. 5C ). Type material Holotype ♂ ( LBM 1430006264 ), dissected with appendages sealed in a glass slide and valves stored dry in a micropalaeontological cavity slide. Collected from the type locality on 5 Dec. 2010 . Allotype ♀ ( LBM 1430006265 ), dissected with appendages sealed in a glass slide and valves stored dry in a micropalaeontological cavity slide. Collected from the type locality on 5 Dec. 2010 . Paratypes 2 ♀♀ ( LBM 1430006266, LBM 1430006267), dissected with appendages sealed in a glass slide and valves stored dry in a micropalaeontological cavity slide. 1 ♂ ( LBM 1430006268), whole, stored dry in a micropalaeontological cavity slide. 1♀ ( LBM 1430006269), valves, stored dry in a micropalaeontological cavity slide. All collected from the type locality on 5 Dec. 2010 . Type locality Sunny Beach in Nishihama, Makino, Lake Biwa, Shiga Prefecture , Japan , 35º27’26.58” N , 136º03’49.5” E . Locality 6 on Fig. 1 . Other material examined 11 ♀♀ , 6 ♂♂ , collected from the type locality on 5 Dec. 2010 . Description Carapace ( Figs 2 A–E, 3A–B) length 1135–1181 µm , height 547–594 µm , female carapace slightly less high than male. Posterior margin more inflated than anterior margin, both margins unevenly curved, with maximum curvature below mid-height. Ventral margin slightly concave, dorsal margin straight, sloping down towards anterior. Dorsal view compressed, with anterior end slightly beak-shaped, posterior end slightly pointed. Calcified inner lamella wider anteriorly than posteriorly. Anterior calcified inner lamella not evenly curved but with convexity in the antero-ventral region, forming distinct wider section (marked with triangle on Fig 3A ). Carapace delicate, with smooth surface. Colour translucent white. Antennule with seven articulated segments ( Fig. 3C ). First segment large, supporting two setae on dorsal edge and two long setae on ventral-apical corner. Second and third segments quadrate, with one dorsal-apical seta each. Fourth and fifth segments each with two long dorsal-apical setae and one short ventral-apical seta. Sixth segment with three long and one short apical setae. Final segment with two long and one short setae, and aesthetasc ya. Fig. 2. A–E . Pseudocandona becca sp. nov. A . Right view of male carapace (LBM 1430006268). B . Right view of female carapace (LBM 1430006269). C . Dorsal view of female carapace, anterior to left (LBM 1430006269). D . Internal view of female left valve (LBM 1430006269). E . Internal view of female right valve (LBM 1430006269). F–I . Pseudocandona atmeta sp. nov. F . Right view of male carapace (LBM 1430006272). G . Right view of female carapace (LBM 1430006273). H . Internal view of female left valve (LBM 1430006274). I . Internal view of female right valve (LBM 1430006274). Fig. 3. Pseudocandona becca sp. nov. A . Internal view of male left valve. B . Internal view of female left valve (LBM 1430006266). C . Male antennule (LBM 1430006264). D . Male antenna (LBM 1430006264). E . Detail of male antenna (LBM 1430006264). F . Detail of female antenna (LBM 1430006266). G . Female mandibular coxa (LBM 1430006267). Scale bar: A–B = 500 µm; C–D, G = 127 µm; E–F = 66 µm. Fig. 4. Pseudocandona becca sp. nov. A . Female mandibular palp (LBM 1430006267). B . Alpha and beta setae of the mandibular palp (LBM 1430006267). C . Female maxillula (setae on endites not drawn) (LBM 1430006265). D–E . Left and right male fifth limb palps (LBM 1430006264). F . Female fifth limb (LBM 1430006265). G . Male sixth limb (LBM 1430006264). H . Male seventh limb (LBM 1430006264). Scale bar: A–C = 66 µm; D–H = 127 µm. Male antenna with second endopodite segment sub-divided ( Fig. 3 D–E). Setae t2 and t3 represented by long male bristles, both similar in morphology, each terminating with small, triangular process. Seta t4 tiny, protruding from near base of t3. Setae z2 and z3 very short, z1 represented by well-developed claw, of similar size to claw G2. Claw G1 about half length of claw G2. Claw Gm on final segment half length of claw GM. Female antennal ( Fig. 3F ) claw G2 about two-thirds length of G1, z1 stout and claw-like, about half length of G2, setae z2 and z3 very short. Claw Gm just over half length of claw GM. Exopodite of both sexes with longest seta relatively short, not reaching to end of first endopodal segment ( Fig. 3D ). Fig. 5. Pseudocandona becca sp. nov. A . Caudal ramus and female genital lobe (LBM 1430006265). B . Hemipenis (LBM 1430006264). C . Detail of lobe h of hemipenis (LBM 1430006264), black triangle marks protrusion (see text for explanation). D . Male caudal ramus (LBM 1430006264). E . Male caudal ramus attachment (LBM 1430006264). Scale bar = 127 µm. Mandibular palp ( Fig. 4A ) with four segments. Alpha seta of first segment short ( Fig. 4 A–B). Inner edge of second segment with 3+1+beta arrangement of setae; beta seta slightly shorter than alpha ( Fig. 4B ). Outer edge of second segment with two apical setae. Third segment with three long sub-apical setae on outer edge, and three long and one short setae arranged along apical edge; outer-most gamma seta, long and without obvious setules. Final segment with robust seta on outer edge, thick claw-like seta in mid-apical position and three shorter setae on inner apical edge. Six long setae on branchial plate. Mandibular coxa ( Fig. 3G ) with five well-developed teeth plus two much smaller, spine-like teeth. Maxillula ( Fig. 4C ) palp first segment with three setae on apical outer margin, and one apical seta offset towards inner edge. Second segment with stepped apical margin, with outer part more distal than inner part. Outer part of apical margin with two long and one mid-length setae, and inner part with three midlength setae. Branchial plate with morphology typical of subfamily, supporting 18–19 normals rays; number of reflexed rays not observed. Fifth limb palps of male asymmetrical ( Fig. 4 D–E). Right palp widens distally to large bulbous hookshaped end, with two sub-apical setae towards inner edge. Left palp distally narrower than right, with finger-like distal hook, and with two sub-apical setae. Fifth limb of female ( Fig. 4 F ) with long d and b setae, and one a seta. Sixth limb ( Fig. 4 G ) five-segmented, with first segment bearing long d1 seta. Setae e and f of second and third segments respectively, both less than half length of next respective segment. Fourth segment with short g seta. Fifth segment with short h1 and h3 seta and well-developed claw h2. Seventh limb ( Fig. 4 H ) with five segments, but division between third and fourth segments weak. First segment with long d1, d2 and dp setae. Second and third segments with no setae. Fourth segment with long g seta. Final segment with two long setae (h2 and h3) and one short, reflexed, hooked setae (h1). Caudal ramus ( Fig. 5 A , D ) with inflated proximal end, tapering distally, slightly curved. Claw Gp approximately 90% length of claw Ga. Seta sa very short. Seta sp short, about 30% length of claw Gp. Male sexual organ ( Figs 5 B , 6 A ) outer lobe (a) tongue-like, elongate with rounded end and striations towards distal edge. Inner lobe (b) wide, slightly sub-triangular, protruding just beyong outer lobe. Medial lobe (h) shorter than both outer and inner lobes, unevenly rounded with triangular to sub-quadrate process on outer edge ( Fig. 5 C ). M-process with rounded proximal part tapering to thin, straight central part, and then expanding to large, elongate, roughly triangular distal part; inner edge with indentation towards base of distal part. Bursa copulatrix (e) elongate and irregular in shape, with distal-most part lobe-like and bent off-axis. Female genital lobe ( Fig. 5 A ) protruding, sub-triangular to rounded. Remarks Smith et al. ( 2014 ) reported that this species, as Pseudocandona sp., has sperm ranging from 424 to 475 µm in length. The combination of the calcified inner lamella with a distinctive convexity in the antero-ventral region of the carapace, the reflexed h1 seta on the seventh limb, and especially the morphology of the hemipenes clearly separate this species from its congeners. The lateral view of the carapace is similar to that of Pseudocandona renoensis ( Gutentag & Benson, 1962 ) and Pseudocandona delormei ( Karanovic, 2006 ) . Pseudocandona renoensis was described from Pleistocene deposits in Kanas, USA ( Gutentag & Benson 1962 ), but later living specimens were reported from Canada ( Delorme 1970 ). The lateral view of the female carapace of P. renoensis is similar to that of the female of Pseudocandona becca sp. nov. , but the male is higher and more rounded posteriorly than the male of Pseudocandona becca sp. nov. Delorme’s ( 1970 ) figure of P. renoensis shows a similar distinctive convexity in the antero-ventral region of the calcified inner lamella of the carapace to that of Pseudocandona becca sp. nov. , but this feature is missing in Gutentag & Benson’s ( 1962 ) original description of P. renoensis . Fig. 6. A . Pseudocandona becca sp. nov. hemipenis (LBM 1430006264). B . Pseudocandona atmeta sp. nov. hemipenis (LBM 1430006270). C . Pseudocandona falcula sp. nov. hemipenis (LBM 1430006275). D . Pseudocandona tenuirostris Hiruta & Mawatari, 2013 hemipenis. E . Undulacandona colymba sp. nov. hemipenis (LBM 1430006279). Pseudocandona delormei was erected by Karanovic ( 2006 ) based on photographs of Pseudocandona hartwigi (G.W. Müller, 1900) and Pseudocandona sarsi (Hartwig, 1899) in Delorme (1970) . Delorme’s specimens of Pseudocandona sarsi were designated by Karanovic ( 2006 ) as the type material of P. delormei , even though she was unable to trace this material. Karanovic’s (2006) opinion that the specimens of Pseudocandona hartwigi and Pseudocandona sarsi figured by Delorme (1970) are the same species is probably incorrect, as the lateral views of the valves of both species are different in Delorme’s figures. Pseudocandona hartwigi figured by Delorme (1970) has an distinctive convexity in the antero-ventral region of the calcified inner lamella, similar to that of Pseudocandona becca sp. nov. (absent in Pseudocandona sarsi and other reports of Pseudocandona hartwigi ), but the carapace in lateral view is noticeably higher than that of Pseudocandona becca sp. nov. and the hemipenes are also differently shaped. Ecology and distribution So far, this species is only known from the type locality. The species was found by digging a small, shallow hole in the sand at the water’s edge (psammon environment) of the beach at Makino in the north-western part of Lake Biwa.