First record and DNA Barcoding of Oman cownose ray, Rhinoptera jayakari Boulenger, 1895 from Andaman Sea, India Author Pradeep, Hosahalli Divakar Author Swapnil, Shivdas Shirke Author Nashad, Musaliyarakam Author Venu, Sasidharan Author Ranjan, Kumar Ravi Author Sumitha, Gopalakrishnan Author Devi, Sukham Monalisha Author Farejiya, Mahesh Kumar text Zoosystema 2018 2018-02-27 40 4 67 74 journal article 10.5252/zoosystema2018v40a4 dcfa8eba-6c43-4f9b-ab56-80c7ebda8574 1638-9387 3764144 urn:lsid:zoobank.org:pub:30D5CE6B-8578-4822-90C1-B51E9937845A Rhinoptera jayakari Boulenger, 1895 Rhinoptera jayakari Boulenger, 1895: 1520 -1523 . MATERIAL EXAMINED. — MUS / FSI /PB/EB/09/2016, 1 ♂ Rhinoptera jayakari of Disc Width (DW) 494 mm and weight 2.9 kg ( Fig 2 A ), collected from Junglighat fishing landing centre, Port Blair , South Andaman . The specimen was caught by a motorized fishing boat with longline gear at a depth of 30-50 m in the Andaman and Nicobar waters of the Andaman Sea. DESCRIPTION OF SPECIMEN FROM THE ANDAMAN SEA Disc length 1.5 in disc width; near straight anterior margin, posterior margin concave, apices acutely pointed ( Fig. 2 ); disc mostly naked, except for small and stellate-based prickles dorsally on snout and inter orbital space ( Fig. 3B ); trunk thick and broad; head relatively broad and its width between posterior margin of spiracles 5.3 in disc width; upper part of the snout bilobed, separated almost at level of mouth from a lower, forward-projecting and also the bilobed part by a continuous deep groove around front of head; eyes moderately large, lateral on head and just anteromedial to spiracles ( Fig. 2B ), eyes 1.8 in spiracle length; mouth transverse, straight, and narrow ( Fig. 3A ), preoral distance 8.4 in disc width; anterior nasal flaps long medially expanded and fused into a broad, elongated fringed nasal curtain overlapping mouth; nostrils slightly anterior to mouth, connected by broad nasoral grooves with mouth ( Fig. 3A ); tail short, 1.4 in disc length; base of tail with relatively high, rounded dorsal fin originating over pelvic-fin insertions; posterior margin of the dorsalfin almost upright; a serrate spine on tail behind dorsal fin ( Fig. 3D ), its base beneath dorsal-fin inner margin; teeth flat and pavement-like, in 9 series in upper jaw ( Fig. 3C ), 3 medial rows of broad teeth and 3-5 rows of narrower diamond-shaped teeth on each side. Colour Dorsal surface of disc dark olivaceous, ventral surface of disc white, tail dark olivaceous gradually becoming black distally. DNA barcoding The obtained partial sequence of COI gene of was submitted to the NCBI database with an accession number KY574494 . FIG. 2. — A , Dorsal view of Rhinoptera jayakari Boulenger, 1895 ; B , antero-lateral view of the head with upper and lower lobes. Size of specimen: 494 mm DW. TABLE 1. — Morphometric details of Rhinoptera jayakari Boulenger, 1895 (in mm and % Disc Width [DW]) in comparison with previous records.
SMF SMF KAUMM SMF SMF KAUMM
34988 34989 36 Present 34988 34989 36 Present
Yemen Jizan Jizan specimen Yemen Jizan Jizan specimen
%DW %DW %DW %DW mm Internasal width, 9.4 9.9 9.3 9.72 48
Disc width 727 684 771 494 anteriorly (between
bases of nasal curtain)
Total length, from medial 100.8 108.2 91.4 111.13 549
Internasal width, 11.3 11.7 11.2 11.74 58
point of snout
posteriorly (between
Tail length 44 45.3 36.5 47.77 236
posterior out ends of
Disc length (direct) 61.5 66.7 62.1 64.57 319
nostrils)
(snout to rear edge of
Width 1 st gill slit 2.8 2.8 2.6 2.83 14
pectoral fin along axis)
Width 5 st gill slit 1.9 1.9 1.7 1.82 9
Disc thickness 11.7 11.7 11.9 11.34 56
Distance between 1 st gill 18.2 18.5 18.8 18.62 92
Disc, anterior margin of 49.1 53 51 55.87 276
slits
spiracle to pectoral
Distance between 5 st gill 12.3 13 12.6 12.75 63
insertion
slits
Disc, snout to maximum 36.9 37.4 36.4 38.87 192
Pelvic fin, length 13.8 13.5 11.5 13.66 67.5
width
Pelvic fin. Base width 6.3 6.7 5.8 6.56 32.4
Head length 26.7 28.2 26.1 26.52 131
Tail width, axil of pelvic fin 3.5 3.5 3.2 3.36 16.6
Snout-preobital 11.2 10.9 11.5 10.73 53
Tail width, base of sting 2 1.9 1.8 1.82 9
Snout preoral 11 11.5 10.8 11.94 59
Tail height, base of sting 2.3 2.1 2.2 2.15 10.3
Snout-prenasal 11.9 11.4 12 11.34 56
Spine length broken 7.3 7.1 0.00broken
Lower-flap length 12.8 12.4 12.8 12.35 61
Eye Diameter 2.3 2.3 2.2 2.23 11 Snout to origin of cloaca 52.4 58.8 53.6 58.50 289
Inter-eye width 17.5 17.7 17 17.61 87 Cloaca origin to sting 11.5 13.1 11.4 12.45 61.5
Orbit and spiracle length 8.5 9.4 8.5 9.31 46
Cloaca origin to tail tip 48.8 49.1 40 48.79 241
Spiracle length 4.2 4.2 4 4.05 20
Interspiracular width, 15.5 16.2 15.6 15.99 79 Cloaca length 3.6 5.1 2.7 4.86 24
anteriorly Clasper, post cloacal 14.6 13.5 13.4 13.36 66
Interspiracular width, 18.2 19.1 18 19.03 94 length (from posterior
posteriorly edge of cloaca to tip
Mouth width, between 8.4 8.8 8.5 8.70 43 of clasper)
edges of teeth Clasper, length from 12.9 13.1 13 12.75 63
Mouth width, between 13.1 13.1 13.2 13.16 65 pelvic axil (from
folders insertion of pelvic fin
Nostril length 1.5 Nasal curtain width 14.7 1.3 15.3 1.4 14.6 1.21 15.38 6 76 to tip of clasper) Dorsal-fin base length 5.8 7 6.6 6.48 32
Dorsal-fin height 2.3 5.3 5.3 5.16 25.5
Nasal curtain length 4.7 4.85 4.4 4.86 24 Teeth rows on upper jaw 7 9 9 9
FIG. 3. — Rhinoptera jayakari Boulenger, 1895 : A , underside of the head; B , location of prickles on dorsal surface of the head; C , nine series of teeth, upper jaw; D , dorsal fin along with spine. Size of specimen: 494 mm DW. The partial sequence of mitochondria COI gene produced a mean value of 623 nucleotide base pairs. BLAST result shows a 99 % match with the existing COI sequences of R. jayakari from India (GenBank: KF 899679.1, KF 899683.1, and KM 0703015.1) in the NCBI database. It also forms a distinctive clade in Neighbour Joining (NJ) phylogenetic tree inferred from mitochondrial COI gene sequences ( Fig. 4 ). The pair wise genetic distances values (K2P) based on COI sequences are given in Table 2 . The interspecies distance ranged from 0.002 to 0.008 and the genetic distance within the genus Rhinoptera ranged from 0.002 to 0.093.