Systematics of the freshwater leech genus Hirudinaria Whitman, 1886 (Arhynchobdellida, Hirudinidae) from northeastern Thailand Author Tubtimon, Jaruwan Author Jeratthitikul, Ekgachai Author Sutcharit, Chirasak Author Kongim, Bangon Author Panha, Somsak text ZooKeys 2014 452 15 33 http://dx.doi.org/10.3897/zookeys.452.7528 journal article http://dx.doi.org/10.3897/zookeys.452.7528 1313-2970-452-15 0EC15CFA330840848804D6F927DEF0D0 Taxon classification Animalia Arhynchobdellida Hirudinidae Hirudinaria javanica (Wahlberg, 1856) Figs 2A, 3A, 4A, 5 A-C Sanguisuga javanica Wahlberg, 1856: 233. Type locality: Samarang, Java [Semarang, Central Java, Indonesia]. Hirudinaria javanica - Whitman 1886 : 373-376, pl. 20, fig. 56. Limnatis (Poecilobdella) javanica - Blanchard 1897 : 349-351, text figure 7. Limnatis javanica - Kaburaki 1921 : 711. Hirudinaria javanica - Moore 1927 : 210-218, figs 50-52. Material examined. Ban Donsala, Na Wa, Nakhon Phanom: CUMZ 3402 (17 specimens), 3404 (18 specimens; Figs 3A, 4A, 5 A-C ), 3429 (9 specimens). Ban Nongwang, Tao Ngoi, Sakon Nakhon: CUMZ 3413 (9 specimens). Ban Janpen, Tao Ngoi, Sakon Nakhon: CUMZ 3415 (16 specimens). Ban Nonghai, Khamchaee, Mukdahan: CUMZ 3422 (4 specimens), 3424 (9 specimens; Figs 2A, 6 A-B ). Chaturaphak Phiman, Roi Et: CUMZ 3419 (1specimen). Figure 4. Illustrations of the reproductive system of A Hirudinaria javanica CUMZ 3404 from Nakhon Phanom, B Hirudinaria manillensis CUMZ 3403 from Nakhon Phanom, and C Hirudinaria sp. CUMZ 3405 from Nakhon Phanom. Abbreviations are: ag = albumin gland, at = atrium, cod = common oviduct, eb = ejaculatory bulb, ep = epididymis, g = ganglion, o = ovary, ps = penis sheath, vas = vas deferens, vc = vagina sac, vd = vagina duct. Figure 5. SEM images of the jaws of A-C Hirudinaria javanica CUMZ 3404 from Nakhon Phanom D-F Hirudinaria manillensis CUMZ 3403 from Nakhon Phanom, and G-I Hirudinaria sp. CUMZ 3405 from Nakhon Phanom. (A, D, G) overall jaw, (B, E, H) each jaw characteristic, and (C, F, I) salivary papillae. Figure 6. Meiotic and mitotic metaphase chromosome spreads of A, B Hirudinaria javanica (n = 13, 2n = 26) CUMZ 3424 from Mukdahan C, D Hirudinaria manillensis (n = 12, 2n = 24) CUMZ 3407 from Mahasarakham, and E, F Hirudinaria sp. (n = 14, 2n = 28) CUMZ 3406 from Nakhon Phanom. Description. In preserved specimens body length 41-184 mm, width 5-16 mm. In live specimens, dorsal side olive green, dark green or yellow brown. Middle dorsal line distinct, black, continuous, parallel with two series of black spots on both sides, two faint black stripes present on each side. Body margin yellow with one ordered series of black spots. Ventral side green without marker. Jaw trignathous, approximately 134 teeth. Number of salivary papillae, both small and large, is 43 glands (Fig. 5 A-C ). Gonopores separated by seven annuli. Male reproductive system located in middle of body between somites XI and XIII. Ejaculatory bulbs short and small. Ejaculatory ducts long, connect with atrium side in somite XI. Atrium short, small, pear-shaped with unclear penis sheath. Vas deferens straight, runs along almost entire body, with 11 testisac pairs (12 pairs in some specimens). Nerve cord runs along body length on right side of atrium. Ovisacs stout, albumin gland not well developed, common oviduct short, opens into female bursa. Vagina caecum short, ovate in shape, no vaginal stalk (Fig. 4A).