Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of seven new species Author Guglya, Yuliia text Zootaxa 2021 2021-08-03 5014 1 1 158 http://dx.doi.org/10.11646/zootaxa.5014.1.1 journal article 10.11646/zootaxa.5014.1.1 1175-5326 5158589 63EEF5A6-EAE0-438F-87BC-AF5806BD3641 Phytomyza veronicicola Hering ( Figs. 278–283 , 674–679 ) Material examined: Ukraine : Kharkiv Region : near Petrivske , 49°10’N , 36°58’E , 17.vi.2019 , 17.vi.2020 , 3– 16.vii.2020 and 24.vii.2011 , Yu. Guglya , ex Veronica longifolia ( 6♂ 3♀ ) ; Sumy Region : Kuzemin , The National Nature Park ”Getmanskyi”, 50°08’N , 34°40’E , 22.v.2010 and 29.v.2011 , Yu. Guglya ( 3♂ ) . Hosts. Plantaginaceae : Veronica L. ( Benavent-Corai et al . 2005 ). Mine. The larva initially forms an upper surface secondary blotch mine ( Fig. 278 ), later moving to the underside of the leaf and feeding as an epidermal miner ( Fig. 279 ). The fully developed mine appears silvery-green from the underside and yellowish from the upper side of the leaf. Pupation takes place within the mine on the underside of the leaf ( Fig. 280 ). Puparium. ( Figs. 281–283 ) Beige, translucent, with brown anterior and posterior spiracles, 2.0 mm long, with distinct segmentation; surface finely wrinkled except for wide bands of minute spines. Posterior spiracles set on short, stout, conical protuberances and entirely separate; with 13 sessile bulbs set arranged in an open ring. Anal plate not protruding above the surface of the puparium viewed from the side and directed posteriorly. Cephalopharyngeal skeleton. ( Fig. 674 ) Mouthhooks equal in size, each bearing two accessory teeth. Intermediate sclerite long, slightly curved, with small, sharp protuberance located ventrally; sclerite 1.24× as long as maximum height of mouthhook. The mouthhook and intermediate sclerite are strongly sclerotized and the pharyngeal sclerite much less so. The ventral cornu bears a narrow “closed” window located posteriorly. Indentation index 83. Female head. ( Figs. 675, 676 ) Dark yellow, with only antenna, palpus and postgena black; orbit projecting above eye in profile; 2 orb s, 1 fr s; lunule of medium height, broad, semicircular, not reaching the level of fr s; pped large, rounded; arista distinctly widened only in basal third; gena medially 0.37× as high as maximum height of eye. Female genitalia. ( Figs. 677–679 ) Capsule of spermatheca medium-sized, 0.27× as high as height of anterior part of oviscape. Spermathecae equal in size, dark brown, oval, higher than wide, with surface corrugated. Internal duct invagination trapezoid, 0.68× as deep as height of spermatheca. Spermathecal duct weakly sclerotized. Ventral receptacle compressed S-shaped, with well sclerotized tail that is bowl-shaped basally. Body of receptacle cylindrical with slightly curved, stout basal connecting tube, strongly sclerotized, 0.3× as wide as capsule of spermatheca; with narrow opening located on high cylindrical projection, 0.75× as wide as maximum diameter of spherical part of body. Distribution. The Czech Republic , Germany , Hungary , Lithuania , Poland , Switzerland , Turkey ( Papp & Černý 2019 ). Ukraine (first record). Comments. Superficially P. veronicicola is similar to P. crassiseta Zetterstedt , which also develops in leaf mines on Veronica species. Spencer (1976) and Papp & Černý (2019) noted a lanceolate arista as a distinctive fea- ture of P. crassiseta , but in specimens from Ukraine only three females have a lanceolate arista and others have only thickened ones, indistinguishable from the basally thickened arista of P. veronicicola . However, in all specimens of P. veronicicola areoles of the frontal setae are yellow and those of the orbital setae are slightly darkened ( Fig. 675 ), whereas all P. crassiseta have a black spot at the base of all fronto-orbital setae ( Figs. 673 ). Thus, colour of the areole is an additional reliable diagnostic feature.