Dyella dinghuensis sp. nov. and Dyella choica sp. nov., isolated from forest soil Author Ou, Fang-hong Author Gao, Zeng-hong Author Chen, Mei-hong Author Bi, Jie-yi Author Qiu, Li-hong text International Journal of Systematic and Evolutionary Microbiology 2019 2019-05-01 69 5 1496 1503 http://dx.doi.org/10.1099/ijsem.0.003356 journal article 20536 10.1099/ijsem.0.003356 00bec356-cfff-4cf1-b6c4-510525aa49ca 1466-5034 6223905 DESCRIPTION OF DYELLA CHOICA SP. NOV. Dyella choica [cho′ i.ca. L. fem. adj. choica (from Gr. adj. choicos ) of earth]. Cells are Gram-stain-negative, aerobic, non-motile, and rod-shaped (0.4–0.6×0.7–2.1 µm). Colonies are circular, convex with clear margin and yellow-coloured after 4 days on R2 A agar. Growth was observed on trypticase soy agar, R2 A agar and Luria – Bertani agar, but not MacConkey agar. Growth on R2 A agar occurs at 12–37 Ǫ C, pH 4.5–6.5 and in the presence of 0–1.0 % (w/v) NaCl. Optimum growth occurs at 28 Ǫ C, pH 6.0 and without NaCl supplement. Catalase-positive and oxidase-positive. Nitrate is not reduced to nitrite. Casein is hydrolysed, but gelatin, starch and Tween 80 are not. H 2 S and indole are not produced. The following substrates can be used as sole carbon sources for growth: capric acid, D- arabinose, D- galactose, D- glucose, D- mannose, maltose, sucrose, trehalose, turanose, D- lyxose, D- fucose, glycogen, inulin, N -acetyl-D- glucosamine and xylitol. The following substrates are not utilized for growth: aesculin ferric citrate, amygdalin, arbutin, capric acid, D- adonitol, D- arabitol, cellobiose, D- fructose, D- fucose, D- gentiobiose, lactose, D- lyxose, D- mannitol, melezitose, melibiose, raffinose, D- ribose, D- sorbitol, D- tagatose, dulcitol, erythritol, glycerol, inositol, L- arabinose, L- arabitol, L- rhamnose, L- sorbose, L- xylose, malic acid, methyl Oi -D- glucopyranoside, methyl Oi -D- mannopyranoside, methyl β -D- xylopyranoside, phenylacetic acid, potassium 2-ketogluconate, potassium 5- ketogluconate, potassium gluconate, salicin and starch ortrisodium citrate. The following enzyme activities are positive: alkaline phosphatase, acidphosphatase, arginine dihydrolase, Oi -galactosidase, Oi -glucosidase, Oi -mannosidase, Oi - fucosidase, β -galactosidase, β -glucosidase, cystine arylamidase, esterase (C8), leucine arylamidase, N -acetyl- β -glucosaminidase and valine arylamidase. Esterase (C4) is weakly positive. The following enzyme activities are negative: a - chymotrypsin, lipase (C14), naphthol-AS-BI-phosphohydrolase and trypsin. Ubiquinone-8 is the respiratory quinone, and iso-C 15: 0 , iso-C 16: 0 , iso-C 17: 1 Ɯ 9 c are the major cellular fatty acids (>10 %). The G +C content of the genomic DNA is 61.7 mol%. The major polar lipids consist of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified aminophospholipid, two unidentified aminolipid and one unidentified phospholipid. The type strain, 4 M-K27 T (=GDMCC 1.1189 T =LMG 30267 T ), was isolated from forest soil sampled at Dinghushan Biosphere Reserve , Guangdong Province , PR China . The 16S rRNA gene sequence and whole genome sequence of strain DHOA06 T have been deposited in DDBJ/EMBL/ GenBank under accession numbers KY433857 and RYYV00000000, respectively.