New and rare lichens of the family Megasporaceae discovered in Poland Author Szczepańska, Katarzyna 0000-0002-7752-3024 Department of Botany and Plant Ecology, Wrocław University of Environmental and Life Sciences, pl. Grunwaldzki 24 a, PL- 50 - 363 Wrocław, Poland. & katarzyna. szczepanska @ upwr. edu. pl; https: // orcid. org / 0000 - 0002 - 7752 - 3024 katarzyna.szczepanska@upwr.edu.pl Author Kukwa, Martin 0000-0003-1560-909X Department of Plant Taxonomy and Nature Conservation, Faculty of Biology, University of Gdańsk, Wita Stwosza 59, PL- 80 - 308 Gdańsk, Poland. & martin. kukwa @ ug. edu. pl; https: // orcid. org / 0000 - 0003 - 1560 - 909 X martin.kukwa@ug.edu.pl Author Guzow-Krzemińska, Beata 0000-0003-0805-7987 Department of Plant Taxonomy and Nature Conservation, Faculty of Biology, University of Gdańsk, Wita Stwosza 59, PL- 80 - 308 Gdańsk, Poland. & beata. guzow-krzeminska @ ug. edu. pl; https: // orcid. org / 0000 - 0003 - 0805 - 7987 beata.guzow-krzeminska@ug.edu.pl Author Urbaniak, Jacek 0000-0002-1300-0873 Department of Botany and Plant Ecology, Wrocław University of Environmental and Life Sciences, pl. Grunwaldzki 24 a, PL- 50 - 363 Wrocław, Poland. & jacek. urbaniak @ upwr. edu. pl; https: // orcid. org / 0000 - 0002 - 1300 - 0873 jacek.urbaniak@upwr.edu.pl text Phytotaxa 2023 2023-05-23 598 2 133 144 http://dx.doi.org/10.11646/phytotaxa.598.2.3 journal article 10.11646/phytotaxa.598.2.3 1179-3163 7961063 Aspicilia goettweigensis (Zahlbr.) Hue Nouv. Arch. Mus . Hist. Nat., Paris, 5 ser. 2(1): 112. 1910 ≡ Lecanora goettweigensis Zahlbr., Ann. Naturhist. Mus . Wien 20: 345. 1905 . Fig. 1A . Thallus lichenized, crustose, squamulose-areolate, olive-brown to olive-grey, matt. Areoles thin, flat and smooth at the margin of the thallus to thick and forming small squamules in the centre. Squamules irregular, white pruinose on the edges, partially erected and overlapping, convex and ear-shaped with medulla visible from underneath, 0.5–0.8 mm in diam. Prothallus rarely visible, fibrous, dark olive-grey. Apothecia rare, immersed, 1 to 3 per areole, 0.2–1.0 mm in diam., thalline margin same colour as the thallus, thin, white pruinose and radially incised on the inside edge, disc black, rounded, flat and matt. Hymenium colourless, 80–100 μm tall with submoniliform paraphyses (3–4 globose apical cells), epihymenium green-brown to olive-brown, N+ green intensifying, K+ yellowish brown (Caesiocinerea-green), hypothecium colourless. Asci 8-spored, ascospores hyaline, simple, narrowly ellipsoid, 18–25 × 8–12 μm. Conidia filiform, 14–20 × 1 μm. Chemistry : According to Paukov et al. (2016) , A. goettweigensis contains stictic acid (major) with norstictic and connorstictic acids (both minor) in the medulla. One Polish specimen lacked secondary substances (Hb. Szczepańska 1333), but the BLAST analyses of the nucITS rDNA sequence undoubtedly identified it as A. goettweigensis (see below). Ecology and distribution: Aspicilia goettweigensis is a rare and poorly known species that has been recorded from only a few European countries, including Austria , the Czech Republic , France , Germany , Hungary , Italy and Switzerland ( Paukov et al. 2016 ; Nimis et al. 2018 ; Nimis & Martellos 2022 ). It occurs on basic and ultramafic rocks, including basalt, serpentinite, syenite and porphyry ( Paukov et al. 2016 ). In Poland , the species has been reported from the Sudety Mts in the vicinity of the town of Jelenia Góra ( Nowak & Tobolewski 1975 ) and in the Karpaty Mts in the Orawsko-Podhalańskie Depression ( Bielczyk 2003 ). TABLE 1. GenBank accession numbers of the newly-obtained nucITS rDNA and mtSSU rDNA sequences of specimens presented in the manuscript.
GenBank no.
Species Isolate no. Locality Voucher specimens nucITS mtSSU
Aspicilia goettweigensis 150 Poland, Sudeckie Foothills K. Szczepańska 1092 OP602297
Aspicilia goettweigensis 337 Poland, Sudety Mts K. Szczepańska 1318 OP602304
Aspicilia goettweigensis 339 Poland, Sudety Mts K. Szczepańska 1320 OP602306
Aspicilia goettweigensis 350 Poland, Zachodniosudeckie Foothills K. Szczepańska 1333 OP602307
Aspicilia verrucigera 73 Poland, Kaszubskie Lakeland M. Kukwa 2916 OP602295
Aspicilia verrucigera 19950 Poland, Bory Tucholskie M. Kukwa 19950 OP700455 OP737751
Aspicilia verrucigera 19956a Poland, Bory Tucholskie M. Kukwa 19956a OP700456 OP737752
Sagedia mastrucata 151 Poland, Sudety Mts K. Szczepańska 1095 OP602298
Sagedia mastrucata 154 Poland, Izerskie Foothills K. Szczepańska 1099 OP602299
Sagedia mastrucata 160 Poland, Izerskie Foothills K. Szczepańska 1106 OP602300
Sagedia mastrucata 166 Poland, Sudety Mts K. Szczepańska 1116 OP602301
Sagedia mastrucata 176 Poland, Sudety Mts K. Szczepańska 1186 OP602302
Sagedia mastrucata 226 Poland, Izerskie Foothills K. Szczepańska 978 OP602303
Sagedia mastrucata 338 Poland, Sudety Mts, K. Szczepańska 1319 OP602305
Sagedia zonata F13 Poland, Sudety Mts K. Szczepańska 41 OP602290
Sagedia zonata 44 Poland, Karpaty Mts KRAM L-42336 OP602291
Sagedia zonata 48 Poland, Karpaty Mts KRAM L-42339 OP602292
Sagedia zonata 59 Poland, Karpaty Mts J. Kiszka s.n. (KRAP) OP602293
Sagedia zonata 60 Poland, Karpaty Mts J. Kiszka, R. Kościelniak s.n. (KRAP) OP602294
Sagedia zonata 132 Poland, Sudety Mts K. Szczepańska 924 OP602296
All of the new A. goettweigensis localities presented in this paper are located in the area of low mountains in Lower Silesia (south-western Poland ). An additional record was found after revision of the Stein’s specimen stored in the WRSL herbarium, which was originally identified as Aspicilia gibbosa (Ach.) Körb. All of the recorded specimens were found growing on silicate rocks in exposed locations, influenced by high insolation and rather low moisture at altitudes of up to 500 m . Notes: Aspicilia goettweigensis can be confused with other members of the genus, especially A. laevata (Ach.) Arnold and A. verrucigera Hue , due to the very similar sizes of the ascospores and conidia, and the secondary chemistry. The most important distinguishing feature of A. goettweigensis , however, is the appearance of the thallus, which consists of characteristic overlapping and convex squamules. In the literature, these squamules are described as hollow, cracking into “popcorn-like tufts”, and showing white medulla in well-developed specimens ( Paukov et al . 2016 ). In addition, unlike A. goettweigensis , A. laevata prefers shady and moist habitats, close to water or located in forests ( Wirth 1995 , Fletcher et al . 2009 ). TABLE 2. The most similar sequences obtained from BLAST analyses of the newly generated nucITS rDNA sequences from specimens analysed in this work. GenBank accession numbers, localities, voucher data and references are given.
Species Locality Voucher specimens References GenBank no. nucITS
Aspicilia goettweigensis Austria J. Vondrák 14026 (PRA, topotype) Paukov et al . 2016 KX159289
Aspicilia goettweigensis Russia AGP20120513-03 (UPS,UFU) Paukov et al . 2016 KX159292
Aspicilia goettweigensis Czech Republic Frolov 51 (herb. I. Frolov, dupl. UPS, UFU) Paukov et al . 2016 KX159293
Aspicilia goettweigensis Czech Republic J. Vondrák 23397 (PRA) Vondrák et al. 2022 OK332934
Aspicilia goettweigensis Czech Republic J. Vondrák 23713 (PRA) Vondrák et al. 2022 OK332935
Aspicilia verrucigera Czech Republic J. Vondrák 24214 (PRA) Vondrák et al. 2022 OK332936
Aspicilia verrucigera Sweden Tibell 22669 (UPS) Nordin et al . 2007 EU057939
Sagedia aff. mastrucata Czech Republic Z. Palice 23193 (PRA) Urbanavichus et al. 2020 MK778583
Sagedia aff. mastrucata Czech Republic Malíček 14741 Malíček 2022 OP730575
Sagedia zonata Sweden Nordin 6219 (UPS) Nordin et al . 2007 EU057943
Sagedia zonata Sweden Nordin 5486 (UPS) Nordin et al . 2007 EU057944
Sagedia zonata Sweden Nordin 5461 (UPS) Nordin et al . 2007 EU057945
Sagedia zonata Norway Owe-Larsson 8942 (UPS) Nordin et al . 2007 EU057946
Sagedia zonata Sweden Owe-Larsson H-254a (UPS) Nordin et al . 2007 EU057947
Sagedia zonata Sweden Nordin 5998 (UPS) Nordin et al . 2007 EU057949
Sagedia zonata Sweden Nordin 5932 (UPS) Nordin et al . 2007 EU057950
Sagedia zonata Sweden Nordin 6035 (UPS) Nordin et al . 2007 EU057951
Sagedia zonata Sweden Nordin 6006 (UPS) Nordin et al . 2007 EU057952
Sagedia zonata Sweden Nordin 5949 (UPS) Nordin et al . 2007 EU057953
The nucITS marker was sequenced from four specimens ( Table 1 ), and the BLAST analyses showed 98% to 100% identity with the sequences of A. goettweigensis deposited in GenBank ( Table 2 , data for A. goettweigensis ). Sequences of the best hits originated from Russia and the Czech Republic (KX159292 and KX159293, respectively). Material examined: POLAND . Dolnośląskie province : Sudety Mts , Jeleniogórska Basin , Łomnickie Hills , Witosza Hill near Staniszów village , elev. 484 m , on granite rock, March 1882 , B. Stein ( WRSL-5791 ); Sudety Mts , Opawskie Mts , Karolinki rocks near Jarnołtówek village , elev. 400 m , on natural outcrop of siliceous rocks in an oak grove, 18 February 2020 , K. Szczepańska 1318 , 1320 ( Hb. Szczepańska ); Sudeckie Foothills , Strzegomskie Hills , Krzyżowa Hill , elev. 360 m , on basalt rock, 19 May 2013 , K. Szczepańska 1092 , 1093 ( Hb. Szczepańska ); Sudeckie Foothills , Kaczawskie Foothills , Organy Wielisławskie quarry in Sędziszowa village , elev. c. 300 m , on porphyry rocks, 8 May 2020 , K. Szczepańska 1333 ( Hb. Szczepańska ) .