Unraveling Capnodiaceae species in Northern Thailand Author Haituk, Supitchakorn Thungdee Sukanya Author Withee, Patchareeya 0000-0002-4412-6209 Department of Entomology and Plant Pathology, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand & patchareeya _ with @ cmu. ac. th; https: // orcid. org / 0000 - 0002 - 4412 - 6209 patchareeya_with@cmu.ac.th Author Cheewangkoon, Ratchadawan 0000-0001-8576-3696 Department of Entomology and Plant Pathology, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand & ratchadawan. c @ cmu. ac. th; https: // orcid. org / 0000 - 0001 - 8576 - 3696 ratchadawan.c@cmu.ac.th Author Suwannarach, Nakarin 0000-0002-2653-1913 Research Center of Microbial Diversity and Sustainable Utilization, Chiang Mai University, Chiang Mai 50200, Thailand & suwan _ 461 @ hotmail. com; https: // orcid. org / 0000 - 0002 - 2653 - 1913 suwan_461@hotmail.com Author Marasinghe, Diana S. 0000-0002-3805-5280 Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand & dianasandamali 91 @ gmail. com; https: // orcid. org / 0000 - 0002 - 3805 - 5280 dianasandamali91@gmail.com Author Hongsanan, Sinang 0000-0003-0550-3152 Research Center of Microbial Diversity and Sustainable Utilization, Chiang Mai University, Chiang Mai 50200, Thailand & Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Science and Oceanography, Shenzhen University, Shenzhen 518060, People’s Republic of China & Office of the Research Administration, Chiang Mai University, Chiang Mai 50200, Thailand & sinang 333 @ gmail. com; https: // orcid. org / 0000 - 0003 - 0550 - 3152 sinang333@gmail.com text Phytotaxa 2023 2023-10-13 620 2 143 156 http://dx.doi.org/10.11646/phytotaxa.620.2.2 journal article 273140 10.11646/phytotaxa.620.2.2 e54f2cb4-c263-4ffc-af17-b75af0d2ec45 1179-3163 10011254 Phragmocapnias betle (Syd., P. Syd. & E.J. Butler) Theiss. & Syd., Annls mycol. 15(6): 480 (1918) [1917] Index Fungorum number: IF151279; FIGURE 5 Colonies saprobic , sooty mold-like, growing on leaves of Rosa × damascena ( Rosaceae ) ( FIGURE 5a, b ). Thallus , dark mycelium growing cover leave surface, black, pelliculose. Mycelium superficial or immersed, hyaline to brown, branched, hyphae smooth, thin-walled, septate ( FIGURE 5g ). Ascomata 90–100 high × up to 120 μm diam. ( x = 117 × 98 μm, n = 5), scattered, subglobose to broadly ellipsoidal, firmly attached to the radiating basal hyphae, pale brown, thick-walled, with ostiole when mature. Peridium 14–18 μm wide, pale to dark brown, cells arranged in a textura angularis ( FIGURE 5f ). Asci 38–48 × 17–27 μm ( x = 45 × 23 μm, n = 7), 8-spored, bitunicate, broadly clavate, with short pedicle, ( FIGURE 5h, i ). Ascospores 20–23× 5–6.5 μm ( x = 22 × 6 μm, n = 10), hyaline, 4–5-septate, fasciculate, cylindric clavate, ends rounded, upper cell slightly wider than the lower cell, smooth-walled, some surrounded by tiny sheath ( FIGURE 5j ). Material examined: THAILAND , Chiang Mai Province , on living leaves of Rosa × damascena ( Rosaceae ), 10 October 2022 , S Hongsanan SDBR-CMURS02.1/1= CMUB40027, living culture in SDBR-CMU480 Note: Our sooty mold collection (SDBR-CMU480) was found on living leaves of Rosa × damascena ( Rosaceae ). We could not identify the primary insect responsible for producing the sugar excretions that serve as nutrition resources for the sooty mold. Instead, we observed 1–2 black garden ants moving around the leaves. In the phylogenetic analysis ( FIGURE 1 ), this strain grouped with other strains of Phragmocapnias betle (CPC 17762, CPC 20476, CPC 21379, and MFLUCC10-0053), with 88% ML/ 1.00 PP support.The morphological characteristics of SDBR-CMU480 are identical to those epitype specimens of Ph. betle described by Chomnunti et al. (2011) . However, our strain SDBR-CMU480 has larger ascomata (90–100 × up to 120 μm diam. vs. 82–93 × 84–105 μm diam.). The size of asci and ascospores in our strain resemble those of the epitype specimen (asci: 38–48 × 17–27 μm vs. 43–53 × 13–33 μm, ascospores: 20–23× 5–6.5 μm vs. 20–24 × 4.8–5.8 μm, Chomnunti et al. 2011 ). Based on the morphological characteristics and phylogenetic analyses, we identified our collection as Ph. betle and this is the first report of Ca. coartatum from Rosa × damascena ( Rosaceae ).