Unraveling Capnodiaceae species in Northern Thailand Author Haituk, Supitchakorn Thungdee Sukanya Author Withee, Patchareeya 0000-0002-4412-6209 Department of Entomology and Plant Pathology, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand & patchareeya _ with @ cmu. ac. th; https: // orcid. org / 0000 - 0002 - 4412 - 6209 patchareeya_with@cmu.ac.th Author Cheewangkoon, Ratchadawan 0000-0001-8576-3696 Department of Entomology and Plant Pathology, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand & ratchadawan. c @ cmu. ac. th; https: // orcid. org / 0000 - 0001 - 8576 - 3696 ratchadawan.c@cmu.ac.th Author Suwannarach, Nakarin 0000-0002-2653-1913 Research Center of Microbial Diversity and Sustainable Utilization, Chiang Mai University, Chiang Mai 50200, Thailand & suwan _ 461 @ hotmail. com; https: // orcid. org / 0000 - 0002 - 2653 - 1913 suwan_461@hotmail.com Author Marasinghe, Diana S. 0000-0002-3805-5280 Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand & dianasandamali 91 @ gmail. com; https: // orcid. org / 0000 - 0002 - 3805 - 5280 dianasandamali91@gmail.com Author Hongsanan, Sinang 0000-0003-0550-3152 Research Center of Microbial Diversity and Sustainable Utilization, Chiang Mai University, Chiang Mai 50200, Thailand & Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Science and Oceanography, Shenzhen University, Shenzhen 518060, People’s Republic of China & Office of the Research Administration, Chiang Mai University, Chiang Mai 50200, Thailand & sinang 333 @ gmail. com; https: // orcid. org / 0000 - 0003 - 0550 - 3152 sinang333@gmail.com text Phytotaxa 2023 2023-10-13 620 2 143 156 http://dx.doi.org/10.11646/phytotaxa.620.2.2 journal article 273140 10.11646/phytotaxa.620.2.2 e54f2cb4-c263-4ffc-af17-b75af0d2ec45 1179-3163 10011254 Capnodium gamsii Abdollahz. & Crous , in Abdollahzadeh et al ., Stud. Mycol. 95: 395 (2020) Index Fungorum number: IF833152 ( FIGURE 3 ) Colonies saprobic sooty mold-like, growing on living leaves of Manihot esculenta and Broussonetia papyrifera ( FIGURE 3a, b ). Mycelium developing on the surface of the plant leaves, hyaline to brown in color, branching, thin-walled, septate, and constricted at the septa ( FIGURE 3i ). Sexual morph : Not observed. Asexual morph : Pycnidia 220–360 × 20–55 μm ( x = 258 × 38 μm, n = 10) on M. esculenta , and 223–280 × 25–34 μm ( x = 230 × 30 μm, n = 10) on B. papyrifera , superficial, slender or flask-shaped, simple, occur singly or in groups, medium to dark brown, sometimes slightly greenish, synnematous in structure ( FIGURE 3d–f ). Ostiole surrounded by hyaline hyphae ( FIGURE 3h ), upper cylindrical region tapering to apex. Conidia 4.2–6.6 × 2.5–3.6 μm ( x = 5.9 × 3.0 μm, n = 15) on M. esculenta , and 4.4–5.0 × 2.1–2.6 μm ( x = 4.7 × 2.4 μm, n = 10) on B. papyrifera , oblong to ellipsoid, aseptate, hyaline, with 2-guttules ( FIGURE 3k, i ). Material examined: THAILAND , Payao Province, on living leaf of Manihot esculenta ( Solanaceae ), 29 October 2022 , N Suwannarach SDBR-CMUPTT01.1 = CMUB40025, living culture in SDBR-CMU479 ; Payao Province, on living leaf of Broussonetia papyrifera (Paper mulberry), 29 October 2022 , N Suwannarach SDBR-CMUBPP01.1 = CMUB40024, living culture in SDBR-CMU476 . FIGURE 3 Capnodium gamsii ( a, d, g, l = morphological characters on Manihot esculenta , CMUB 40025; b, e, f, h–k, = morphological characters on Broussonetia papyrifera , CMUB 40024). a, b Sooty mold on the surface of leaves. c Appearance of conidiomata on the host surface. d–f Squash mount of pycnidia. g Pycnidia wall. h Ostiole surrounded by hyaline hyphae. i Network-like hyphae. j Germinating conidia. k, l Conidia. Scale bars: d–f = 50 µm, k–h = 10 µm, i–j = 20 µm. Notes: Our sooty mold collections, SDBR-CMU476 and SDBR-CMU479, were found on living leaves of Broussonetia papyrifera , and Manihot esculenta , respectively. We found a few carcasses of aphids on both plant species. In our phylogenetic analyses ( FIGURE 1 ), both strains grouped with other strains of Capnodium gamsii (CBS 892.73, CBS 146153, CBS 146154, CBS 146155, CBS 146156, and MFLUCC10-0066) (76% ML/ 0.97 PP). The morphological characteristics of SDBR-CMU476 and SDBR-CMU479 are identical to Ca . gamsii . However, SDBR-CMU479 has shorter pycnidia (220–360 × 20–55 μm vs. 210–420 × 22–35 μm) and larger conidia (4.2–6.6 × 2.5–3.6 μm vs. 3.6–8.1 × 1.6–2.9 μm) than those of the type material of Ca . gamsii described by Abdollahzadeh et al. (2020) . Furthermore, SDBR-CMU476 has smaller conidia compared to the type material (4.4–5.0 × 2.1–2.6 μm vs. 3.6–8.1 × 1.6–2.9 μm) ( Abdollahzadeh et al. 2020 ). In addition, our collection, CMUB40025 has a darker brown pycnidia, and CMUB40024 has a slightly greenish to brown pycnidia, while the type material has medium to dark brown pycnidia. Based on the morphology and phylogeny, we identified our collection as Ca . gamsii , and this is the first report of Ca . gamsii from Broussonetia papyrifera and Manihot esculenta .