Three new species of Teunia (Cryptococcaceae, Tremellales) identified through phenotypic and phylogenetic analyses Author Guo, Qi-Chao https://orcid.org/0009-0002-9245-479X School of Life Science and Agricultural Engineering, Nanyang Normal University, Nanyang 473061, China Author Liu, Shan https://orcid.org/0009-0003-2845-1495 School of Life Science and Agricultural Engineering, Nanyang Normal University, Nanyang 473061, China Author Qiao, Ya-Zhuo https://orcid.org/0009-0000-9074-2443 School of Life Science and Agricultural Engineering, Nanyang Normal University, Nanyang 473061, China Author Hui, Feng-Li 0000-0001-7928-3055 School of Life Science and Agricultural Engineering, Nanyang Normal University, Nanyang 473061, China text MycoKeys 2024 2024-05-15 105 139 153 journal article 296098 10.3897/mycokeys.105.120534 9f0d4a25-9118-4e26-97a5-ec33b6259f22 Teunia qingyuanensis Y. Z. Qiao & F. L. Hui sp. nov. Fig. 3 B Etymology. Referring to the locality, Qingyuan Mountain, where the type strain was collected. Typus . China . Fujian Prov. Quanzhou City , Qingyuan Mountain ; in rotting wood ; Mar 2022 ; W. T. Hu & S. B. Chu ; NYNU 22475 ( holotype GDMCC 2.294 T , culture ex-type PYCC 9929 ; GenBank Nos: OP 269841 , OP 269842 , PP 236728 ) . Description. On YM agar after seven days at 20 ° C, the streak culture was cream, mucoid and smooth, with an entire margin. After seven days in YM broth at 20 ° C, single cells were ovoid to ellipsoidal, 3–7 × 4–7.5 μm, budding polar. After one month at 20 ° C, sediment was present. In Dalmau plate culture on CMA , no hyphae or pseudohyphae were formed. Sexual structures were not observed on PDA , CMA or V 8 agar. Glucose fermentation was absent. Glucose, inulin, sucrose, raffinose, melibiose, galactose, lactose, trehalose, maltose, melezitose, cellobiose, salicin, L-sorbose (weak), L-rhamnose, D-xylose, L-arabinose, D-arabinose, 5 - keto-D-gluconate, D-ribose, ethanol, glycerol, ribitol, galactitol, D-mannitol, D-glucitol, myo-inositol, DL-lactate, succinate, D-gluconate, 2 - keto-D-gluconate, D-glucuronate, and glucono- 1, 5 - lactone were assimilated as carbon sources; methanol, erythritol, and D-glucosamine were not assimilated. Ethylamine and L-lysine were assimilated as nitrogen sources; nitrate, nitrite, and cadaverine were not assimilated. Maximum growth temperature was 30 ° C. Growth in vitamin-free medium was positive. Growth on 50 % (w / w) glucose-yeast extract agar was negative. Starch-like substances were not produced. Urease activity and Diazonium Blue B reaction were positive. Note. In the molecular analysis (Fig. 2 ), T. qingyuanensis sp. nov. formed a distinct clade together with T. cuniculi . They can be differentiated by the ability of T. qingyuanensis sp. nov. to grow in vitamin-free medium and to assimilate raffinose, melibiose, and DL-Lactate.