Two new species of Sabellariidae (Annelida, Polychaeta) from the abyss of eastern Australia Author Zhang, Jinghuai 0000-0003-4745-6462 South China Sea Environmental Monitoring Center, State Oceanic Administration, 155 Xingangxi Road, Guangzhou, P. R. China Australian Museum Research Institute, Australian Museum, 1, William Street, 2010, New South Wales, Australia. & Department of Biological Sciences, Macquarie University, North Ryde 2109, Australia & zhangjinghuai @ sohu. com; https: // orcid. org / 0000 - 0003 - 4745 - 6462 zhangjinghuai@sohu.com Author Hutchings, Pat Department of Biological Sciences, Macquarie University, North Ryde 2109, Australia Author Burghardt, Ingo 0000-0001-5251-1708 Ingo. burghardt @ austmus. gov. au; https: // orcid. org / 0000 - 0001 - 5251 - 1708 ngo.burghardt@austmus.gov.au Author Kupriyanova, Elena 0000-0003-0336-4718 Department of Biological Sciences, Macquarie University, North Ryde 2109, Australia & Elena. kupriyanova @ austmus. gov. au; https: // orcid. org / 0000 - 0003 - 0336 - 4718 lena.kupriyanova@austmus.gov.au text Zootaxa 2020 2020-08-03 4821 3 487 510 journal article 8799 10.11646/zootaxa.4821.3.4 30186aa6-d5ef-4204-907b-1b6bc37af396 1175-5326 4401032 F5D6CEBE-B8BC-4F73-85F7-EB3698A69512 Gesaia csiro n. sp. Figures 2–5 , Table 2 Material examined. Holotype with tube, St. 89 , 30º15.798´– 30º17.358´S , 153º51.522´– 153º50.628´E , 4414–4436 m , Brenke epibenthic sledge, 6 th June 2017 , AM W.49506. Paratypes : St. 88 , 30º15.840´– 30º17.202´S , 153º52.2´– 153º49.812´E , 4401–4481 m , beam trawl, 6 th June 2017 , AM W.50680, 140 specs ; AM W.50681, 12 specs, W.50681.001 (tissue sample taken). St. 89 , 30º15.798´– 30º17.358´S 153º51.522´– 153º50.628´E , 4414–4436 m , Brenke epibenthic sledge , 6 th June 2017 , AM W.52780, 4 specs ; AM W.50680.001 to AM W.50680.004, 4 dry specs, mounted on SEM stubs. Other material examined : St. 89 , 30º15.798´– 30º17.358´S 153º51.522´– 153º50.628´E , 4414–4436 m , Brenke epibenthic sledge, 6–7 th June 2017 , AM W.50678, 29 specs; AM W.50679, 11 specs (tissue sample taken); St.128 , 23°37.8667’S 154°39.5833’E , 1761–1770 m , 13 th June 201, beam trawl, AM W.50677, 7 specs (tissue sample taken). Description . Holotype white after preservation in alcohol. Body tapering from abdomen to cauda, long cauda folded back onto ventrum. Holotype 14.5 mm in body length (length of paleae 4 mm , of operculum and thorax 2.5 mm , of abdomen 6 mm and of cauda 2 mm ); 1.7 mm maximum body width ( Figs 2A , 3 B–C). FIGURE 2. Line drawings of Gesaia csiro n. sp. holotype (AM W.49506). A. Entire animal, ventral view. B. Anterior end, lateral view. C. Diagramatic presentation of arrangement of paleae on each opercular lobe. D. Anus. Abbreviations: br, branchiae; ca, cauda; ip, inner paleae; mo, medial organ; nec, neuropodial cirrus; nh, nuchal hooks; op, outer paleae; opa, opercular papillae; pa, palp; s, segment; tf, tentacular filaments. FIGURE 3. Photos of Gesaia csiro n. sp. holotype (AM W.49506). A. Anterior end with tube (anterior parts broken), lateral view. B. Entire body, lateral view. C. Entire body, ventral view. D–E. Close-up view of nuchal hooks. F. Anus. Abbreviations: br1, branchia of chaetiger 2; ca, cauda; nh, nuchal hooks; op, outer paleae; opa, opercular papilla; s, segment; tf, tentacular filaments. Operculum completely divided into two elongated lobes with distal ends perpendicular to longitudinal axis ( Figs 3 B–C, 4A). Operculum with 22 pairs of outer paleae, arranged in a circle around opercular lobes ( Fig. 2C ); outer paleae golden, slightly flattened, elongated, ending in fine tips rolled inward ( Figs 2A, C , 3 A–C, 4B). Thecae ornamented, arranged diagonally; basal thecae complete, with slightly serrated distal edges ( Fig. 5A ); thecae of middle part complete, distal edges slightly-expanded with weakly serrated margins ( Fig. 5B ) and with short irregular distal fringes ( Fig. 5C ); thecae of distal part with elongated tips, and margins with heavily serrated margins ( Fig. 5D ). Two inner paleae on each opercular lobe; these paleae straight, circular in cross-section, golden, with smooth surface and tapering tips ( Figs 2C , 4A ). Six pairs of opercular papillae, arranged spirally from nuchal hooks to ventral-inner distal end of operculum ( Figs 2 A–B, 4A–C). A pair of cylindrical nuchal hooks, conspicuously curved without limbation ( Figs 2B , 3B , D–E, 4A). A small medial organ at dorsal junction of opercular lobes ( Fig. 2C ). Eyes absent. Three pairs of tentacular filaments ( Figs 2A , 3C , 4C ). Buccal flaps absent ( Fig. 4C ). Pair of fine long palps extended beyond operculum ( Figs 2A , 4C ). Segment 1 (thoracic) with a pair of long tapering neuropodial cirri and capillary chaetae ( Figs 2 A–B; 4C). Segment 2 (thoracic) with a pair of triangular lateral lobes between dorsal branchia and neuropodia with capillary chaetae, notopodia absent ( Figs 2B , 4A ). Eleven pairs of dorsal tapering branchiae present on chaetigers 2–12, largest ones on chaetigers 3–6, subsequently reducing posteriorly in size ( Figs 2B , 4A ). Segments 3–4 respectively with one ventral lobe, without neuropodial cirri ( Figs 2 A–B, 3B–C, 4A). Segments 3–6 (parathoracic) with two types of chaetae on noto- and neuropodia arranged in transverse rows: notopodia with 5–6 long robust lanceolate chaetae interspersed with few fine capillaries ( Figs 2 A–B, 4A; 5G); neuropodia with about four fine lanceolate chaetae interspersed with several fine capillaries ( Fig. 5 E–F). Notopodial lanceolate chaetae much stouter than neuropodial ones ( Figs 2 A–B, 3B, 5E–G). Notopodial lanceolate chaetae on segment 6 directed more posteriorly than those on segments 3–5. Abdominal notopodial tori decreasing in size posteriorly, with number of uncini per row decreasing respectively. Each uncinus with two longitudinal rows of teeth ( Fig. 5H ). Neuropodia bearing long fine capillaries with fringes of thecae ( Figs 2 A–B, 3B–C). FIGURE 4. SEM of Gesaia csiro n. sp. (AM W.50680.004). A. Anterior end, dorso-lateral view. B. Anterior end, ventral view. C. Close-up view of buccal cavity. Abbreviations: bo, buccal organ; br, branchiae; ip, inner paleae; nec, neuropodial cirrus; nh, nuchal hooks; op, outer paleae; opa, opercular papillae; pa, palp; s, segment; tf, tentacular filaments. TABLE 2. Diagnostic characters of species of Gesaia .

Species	Type locality, depth	Surface of inner paleae	Distal fringe of distal thecae of outer paleae	Distal margin of distal thecae of outer paleae
Gesaia elegans ( Fauvel, 1911 )	Off Madeira, East Atlantic Ocean,1968 m	spiral edged surface of distal- middle portion	present, unexpanded, short	complete, not splayed, spiral
G. fauchaldi Kirtley, 1994	Bermuda Rise, Northwest Atlantic Sea, 4749 m	smooth	present, long and short irregular, expanded	hemithecate, not splayed, horizontal
G. fossae Kirtley, 1994	Mozambique Channel, South Indian Ocean, 4983–5000 m	smooth	present, long, expanded	complete, splayed, horizontal
G. hartmanae Kirtley, 1994	Off South Sandwich Island, Southern Ocean, 2452 m	smooth	present, unexpanded, short	complete, not splayed, horizontal
G. hessi Kirtley, 1994	Drake Passage, Antarctica, Southern Ocean, 4758 m	distal thecal margins, poorly marked	present, expanded, long	complete, not splayed, spiral
G. lanai Kirtley, 1994	East off Pernambuco, Brazil, South Atlantic Ocean,776–805 m	smooth	present, unexpanded, short	hemithecate, splayed, horizontal
G. ryani Kirtley, 1994	Between Prince Edward Island and Crozet Island, South Indian Ocean, 1622 m	smooth (distal thecal margins poorly expressed)	absent	complete, not splayed, horizontal
G. vityazia Kirtley, 1994	Kurile-Kamchatka Trench, Northwest Pacific Ocean, 5790 m	smooth	present, expanded, long	hemithecate, not splayed, horizontal
G. csiro n. sp.	Eastern Australia, South Pacific Ocean, 4414–4436 m	smooth	present, expanded, long irregular	complete, not splayed, horizontal

FIGURE 5. SEM micrographs of Gesaia csiro n. sp. chaetae (AM W.50680.004). A–D. Close-up view of proximal (A), middle (B), middle distal (C), and distalmost parts (D) of outer paleae. E. Neuropodium from segment 4. F. Close-up view of neurochaetae from segment 4. G. Notopodial lanceolate chaetae from parathoracic segment. H. Abdominal notopodial uncini. Cauda fine long, about two third length of abdomen ( Figs 2A , 3 B–C). Anus flat with three appendages on each side ( Figs 2D , 3F ). Tube made of fine sand. Variability . The paratypes are 19.6–30.1 mm in length (measured without cauda), 1.7–2.3 mm in width, have 21–23 chaetigers (only counting specimens with cauda), 15–24 pairs of outer paleae, 2–3 pairs of inner paleae, 5–8 pairs of opercular papillae, and 3–6 pairs of tentacular filaments. Several specimens from AM W.58081 have two pairs of nuchal hooks, an anterior pair of small hooks and a pair of large hooks posteriorly, which suggests that these hooks may be regenerating . Remarks. Gesaia csiro n. sp. differs from congeners by a combination of characters such as smooth surface of inner paleae, distal thecae of outer paleae with long, irregular and expanded distal fringe, distal margin of distal thecae of outer paleae circled and nearly horizontal to the longitudinal axis of blade ( Table 2 ). Gesaia csiro n. sp. has smooth surface of inner paleae and thus differs from G. elegans Fauvel, 1911 , G. hessi Kirtley, 1994 and G. ryani Kirtley, 1994 that are characterized by poorly ornamented surfaces of inner paleae. Gesaia csiro n. sp. has long expanded distal fringe of distal thecae of outer paleae, while G. ryani lacks the distal fringe altogether, and in G. hartmanae Kirtley, 1994 and in G. lanai Kirtley, 1994 this distal fringe is short and unexpanded. Gesaia csiro n. sp. can be distinguished from G. fauchaldi Kirtley, 1994 , G. fossae Kirtley, 1994 , and G. vityazia Kirtley, 1994 by the distal margin of distal thecae of outer paleae: the margin of distal thecae is complete, not splayed and horizontal in G. csiro n. sp. , but G. fauchaldi and G. vityazia have hemithecate distal margins, while G. fossae has complete, splayed distal margin. Kirtley (1994) described seven new Gesaia species from the upper bathyal to lower abyssal zone of the Pacific, Atlantic, South Indian and Southern oceans. Since Kirtley (1994) and until this study, no new species of Gesaia were described, but most of the descriptions of Kirtley (1994) are based entirely on the morphology of the paleae, with few body characters described. Specimens of Gesaia are easily broken at segment 7, and it is difficult to extract entire animals from their tubes as the posterior part of the body is fragile and breaks off. While the characters of the paleae do not get distorted during preservation and are useful to identify these species, a comprehensive review of the genus based on freshly collected material with entire body described would be very useful. Distribution . Off the east coast of Australia from 30º15.798´– 30º17.358´S . The species is known only from type locality ( Fig. 1 ). Habitat. Depths 4401–4481 m . Tubes built of fine sand forming colonies on substrate. Etymology . The species was named after the Commonwealth Scientific and Industrial Research Organisation (CSIRO) that manages and operates R/V “Investigator” as the Marine National Facility. Genus Phalacrostemma Marenzeller, 1895 Type species. Phalacrostemma cidariophilum Marenzeller, 1895 , by monotypy. Type locality. Off Pelagossa Island, Adriatic Sea. Diagnosis. (after Capa & Hutchings 2019 ). Operculum with lobes completely fused to each other and distal disc perpendicular to operculum. Eight to 10 pairs of long and conical papillae around opercular lobes. Tentacular filaments absent or present along margins of buccal cavity. Buccal flaps present (or secondarily absent). One pair of palps similar in length to operculum. Median organ at dorsal junction of opercular lobes. Outer paleae arranged in a spiral, with straight cylindrical or slightly flattened blades with ornamented thecae. Few (two to eight) pairs of inner opercular paleae arranged in a short line on dorsal half of inner margin of opercular lobes, with straight cylindrical or slightly flattened blades with smooth margins. Two to five pairs of nuchal hooks with limbation on concave margin. Neuropodia of segment 1 with one to three pairs of cirri. Thoracic branchiae present. Four parathoracic segments. Parathoracic notopodia with lanceolate chaetae interspersed with capillaries, neuropodia with fine lanceolate chaetae interspersed with capillaries.