Review of the genus Chagasia (Diptera: Culicidae: Anophelinae) Author Harbach, Ralph E. Author Howard, Theresa M. text Zootaxa 2009 2210 1 25 journal article 10.5281/zenodo.189830 7da282e9-8437-4017-9c58-bad9557a9dc1 1175-5326 189830 Chagasia ablusa Harbach , new species Chagasia fajardoi of Komp, 1936 : 66 ( Colombia , Ƥ L P, bionomics); Gast Galvis, 1943 : 5 , 8, 9, 19 ( Colombia , 3 Ƥ L);? Romeo Viamonte & Castro, 1951 : 319 , 324, Fig. 12 (Ƥ*); Gabaldon & Cova-Garcia, 1952 : 179 , 197, 198, Fig. 8H (in part,? Bolivia , Colombia ); Lane, 1953 : 139 –144 (in part, Colombia , 3* Ƥ E* L* P*); Horsfall, 1955 : 41 (distribution, L, bionomics); Stone et al ., 1959 : 10 (in part, catalogue, Colombia ); Cova-Garcia, 1961 : 181 –183 (? Venezuela , A, identification). Chagasia fajardi of Russell et al ., 1943 : 35 , 39, 42 (Ƥ L, bionomics, distribution); Knight & Stone, 1977 : 68 (in part, catalogue, Colombia ); Guimarães, 1997 : 30 (in part, catalogue, Colombia ). Diagnosis. The adults of Ch . ablusa are distinguished from those of other species of Chagasia as follows: front of anterior promontory with yellow scales contiguous and well-contrasted with white dorsocentral scales (distinction from Ch . fajardi ); acrostichal scales pale anteriorly, dark posteriorly (as in Fig. 3 B) (distinction from Ch . bathana and Ch . bonneae ); without pale scales on mesal margin of supraalar scales (distinction from Ch . fajardi ); wing dark-scaled with speckling of pale scales on proximal half of costa (distinction from Ch . bathana and Ch . bonneae ), vein R4+5 with distinct cluster of darker scales at base; hindtibia with distinct semierect clusters of dark scales ( Fig. 4 A) (distinction from Ch . fajardi and Ch . rozeboomi ); hindtarsomeres 2–5 without postbasal dark bands (distinction from Ch . bathana ), basal pale band of hindtarsomere 2 usually very long, 3.17–10.33 (mean = 5.89) length of apical dark band ( Fig. 5 D) (92% distinction from Ch . fajardi ), hindtarsomere 5 with apical dark band (distinction from Ch . bonneae ). Males are distinguished by the presence of a single stout specialised seta on the dorsomesal prominence of the gonocoxite (unique) and fine setae on the claspette (distinction from Ch . bathana and Ch . bonneae ). Larvae are distinguished by the development of certain setae: setae 5- and 7-C are long (distinction from Ch . rozeboomi ); seta 5-C is inserted anterior to the base of the antenna on a line midway between the insertions of setae 4- and 7-C, the rachis (main stem) of 5-C reaches the base of seta 4-C and the distance between the insertions of the 2 seta 5-C is less than the distance between the insertions of setae 4- and 7-C (distinctions from Ch . bathana , Ch . bonneae and Ch . rozeboomi ); setae 11- and 13-C are about the same length and shorter than the antenna, about twothirds as long (distinction from Ch . bathana , Ch . bonneae and Ch . rozeboomi ); seta 15-C is single and long (distinction from Ch . bonneae ); and seta 1-P has long aciculae that arise near the middle of each primary branch (distinction from Ch . rozeboomi ). Pupae have no diagnostic features but they differ from pupae of Ch . bonneae in lacking a ligulate process on the rim of the trumpet. Etymology. The specific name ablusa is the feminine form of the Latin adjective ablusus , which means ‘different’. Discussion. Chagasia ablusa has been misidentified as Ch . fajardi in the past based on superficial similarities of the adults. Whether or not these similarities indicate that Ch . ablusa is more closely related to Ch . fajardi than to the other species of Chagasia is a moot question. The structure of the male genitalia and the darkly scaled wings and posterior dorsocentral area suggest that Ch . ablusa , Ch . fajardi and Ch . rozeboomi are more closely related to one another than either is to Ch . bathana and Ch . bonneae . Until the larva of Ch . fajardi is known with certainty (see below), it is pointless to speculate on relationships based on larval morphology. Because Chagasia is a small homogeneous assemblage of species that exhibit a paucity of anatomical distinctions, molecular data will probably be needed to resolve phylogenetic relationships within the genus. Distribution. Colombia , Peru and? Venezuela . Published reports of Ch . fajardi in Venezuela (see above) probably refer to Ch . ablusa based on its proximity and topographic similarity to Colombia , but this requires confirmation as specimens from Venezuela were not available for study. Chagasia ablusa surely occurs in Ecuador and is likely to be present in western Bolivia . In fact, reports of Ch . fajardi in the latter country (see below) may refer to this species. Material examined. Type series: Twenty specimens (10Ƥ, 13, 13G, 3Le, 3Pe, 2L). HOLOTYPE , female (PE 288-1), with LePe on microscope slide, PERU : Junín , Satipo, Mission Cutivireni (12 S 74 W), 400 m , 10 March 1985 ( Calderone ), WRBU ACC 1131. Paratypes , 1ƤLePe (PE288-4), 13 with dissected genitalia (PE 288-105), 1L (PE 288), same data as holotype ; 5Ƥ (PE 359), same as holotype except 22 March 1985 ( Hayes, Harrison & Savage ); 1Ƥ (PE 357), same as holotype except 21 March 1985 ( Victor Lugo & others ); PE 467- 16 (1ƤLePe), 1L (PE 467a) Madre de Dios , Rio Manu, Pakitza ( 11° 55' 48" S 71° 15' 18" W ), 250 m , 31 October 1990 ( Wilkerson, Gaffigan & Mallampalli ), WRBU ACC 1445. The type series is deposited in the National Museum of Natural History, Smithsonian Institution, Washington, DC, USA . Other material examined: Sixty-one specimens, COLOMBIA , Meta , Restrepo (1Ƥ, 23, 13G), Villavicencio (15Ƥ, 53, 13G, 13Le, 14Pe, 4L); unknown locality (1Ƥ).