Taxonomy and DNA barcoding of the dark-winged fungus gnat genus Zygoneura Meigen (Diptera: Sciaridae) from China, with revision of the type materials Author Shah, Bismillah 0000-0002-8407-8627 Department of Forestry Protection, School of Forestry and Biotechnology, Zhejiang A & F University, 666 Wusu street, Linan, Hangzhou, Zhejiang 311300, P. R. China bismillahshah1990@yahoo.com Author Shao, Yisa 0009-0008-5493-6409 Department of Forestry Protection, School of Forestry and Biotechnology, Zhejiang A & F University, 666 Wusu street, Linan, Hangzhou, Zhejiang 311300, P. R. China 2909187740@qq.com Author Du, Hong 0009-0009-4843-4899 Department of Forestry Protection, School of Forestry and Biotechnology, Zhejiang A & F University, 666 Wusu street, Linan, Hangzhou, Zhejiang 311300, P. R. China 983468026@qq.com Author Wang, Yang 0009-0002-2897-8193 Comprehensive Service Center of Public Institutions in Qiantan Town, Jiande, Zhejiang 311602, P. R. China Author Huang, Junhao 0000-0002-5038-3535 Department of Forestry Protection, School of Forestry and Biotechnology, Zhejiang A & F University, 666 Wusu street, Linan, Hangzhou, Zhejiang 311300, P. R. China huangjh@zafu.edu.cn text Zootaxa 2024 2024-08-20 5496 3 377 400 http://dx.doi.org/10.11646/zootaxa.5496.3.5 journal article 10.11646/zootaxa.5496.3.5 1175-5326 13346901 58D78B70-604A-42F5-86D4-4395CDB17A41 Zygoneura ( Pharetratula) longa Zhang & Wu, 2010 ( Figs 8A–D ) Zygoneura ( Pharetratula ) longa Zhang & Wu, 2010 [ Zhang et al . (2010) : 43 ; Shin et al . (2014) : 562 ; Huang et al . (2020) : 360 ]. FIGURE 8. Zygoneura ( Pharetratula ) longa Zhang & Wu. i: slide, front side; ii: slide, flip side; A: habitus, lateral view; B: head, frontal view; C: hypopygium, ventral view; D: fourth antennal flagellomere. Type locality. China : Zhejiang Province , Lishui . Type Material. Holotype ( 1♂ ) in Coll. ZAFU ( China ). Material examined. Holotype [ SM00948 ], 1♂ , Zhejiang Province , Lishui , Taishun , Mt. Wuyanling nature reserve , 28°01′ N , 120°38′ E , 600 m a.s.l. 04.VIII.2005 , leg. Yi-Ping Wang. Additional description. Male. Head brown, antenna and maxillary palpus stramineous. Eye bridge four facets wide. Face with 6–8 setae. Maxillary palpus with three segments ( Fig. 8B ). Fourth antennal flagellomere 2.5 times as long as wide, body with mostly short setae and with a row of clearly elongated setae near the neck ( Fig. 8D ). Thorax pale brown. Wing membrane without macrotrichia. M 1 , M 2 , CuA 1 , CuA 2 , r-m with no setae. stM faded in the specimen. Legs ( Fig. 8A ) stramineous. Tarsal claws three-toothed. Hypopygium ( Fig. 8C ) yellowish brown. Gonocoxae nearly as long as or slightly longer than gonostyli, ventral medial margin with two distinct groups of setae. Gonostylus ( Fig. 8C ) slender, with a sub-apical and a sub-basal megasetae, inner margin slightly concave, with short fine setae. Tegmen membranous, higher than wide.Aedeagus short, slender ( Fig. 8C ). For detailed description, see Zhang et al . (2010) . Remarks. Only holotype seen, 2♂♂ paratypes [SM00129, SM00507] on loan and not examined in this study. This species resembles Z. ( Pharetratula ) subdivergens (Mohrig & Mamaev) and Z. ( Pharetratula ) divergens (Mamaev) in the subapical and sub-basal megasetae. However, it can easily be distinguished by the distinctly more slender gonostylus ( Fig. 8C ) and two unequal megasetae ( Zhang et al . 2010 ). Distribution. China : Zhejiang , Fujian ( Zhang et al . 2010 ).