Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of new species. I. Subgroups steinboecki and longipes Author Makarchenko, Eugenyi A. Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far East Branch of the Russian Academy of Sciences, 100 let Vladivostoku 159, 690022 Vladivostok, Russia Author Semenchenko, Alexander A. Laboratory of Ecology and Evolutionary Biology of Aquatic Organisms, Far Eastern Federal University, Suhanova St. 8, 690950 Vladivostok, Russia Author Palatov, Dmitry M. 0000-0002-8826-9316 A. N. Severtsov Institute of Ecology and Evolution of the Russian Academy of Sciences, Leninskij prosp. 33, 119071 Moscow, Russia triops @ yandex. ru; https: // orcid. org / 0000 - 0002 - 8826 - 9316 triops@yandex.ru text Zootaxa 2022 2022-04-13 5125 5 483 512 journal article 56018 10.11646/zootaxa.5125.5.2 6a677d3b-0f91-4f11-8cf2-64f4ad2a47d2 1175-5326 6457259 39EBA798-29B1-4450-9085-A4FB6D1DB331 Diamesa moubayedi Makarchenko et Semenchenko , sp. nov. http://zoobank.org/NomenclaturalActs/ ECE88876–308D–4C72–9120–F8DECF3CD8D2 ( Figs. 39–40, 43–44 , 46–47, 52, 55 , 75 ) Diamesa sakartvella Kownacki et Kownacka ; Moubayed & Laville 1983: 222 . Type material. Holotype , adult male, LEBANON : glacial springs and small cold stream located in the upper basin of the Beirut River , Mount Sannine , N 33.9025 , E 35.783889 , 5.VIII.2005 , altitude 2000 m above sea level , leg. J. Moubayed. Paratypes : 6 adult males, the same data as holotype . Derivatio nominis. The species is named in honor of the French chironomid taxonomist Joel Moubayed who collected type material. Description Adult male (n = 5, except when otherwise stated). Total length 3.7–4.1 m . Total length/wing length 1.13–1.23. Coloration. Brown to dark brown. Head, thorax and abdomen brown to dark brown. Legs brown to dark brown. Wings grey, with brownish veins. Head. Eyes hairy, reniform. Temporal setae including 6–8 frontals, 7–9 orbitals, 5–8 verticals and 4–5 postorbitals. Frontal tubercles 28 μm height. Clypeus with 5–11 setae. Antenna with 8 flagellomeres and reduced plume of setae ( Fig. 39 ); number and length of these setae on 1–7 flagellomeres respectively (n=2): 3 (32–40 μm), 3 (56–68 μm), 3 (64–68 μm), 0, 2 (60–64 μm) 0, 0; terminal flagellomere with 5 setae, 80–88 μm long in basal part and with 2 subapical setae, 20–24 μm long. Length of 1–8 flagellomeres (μm): 100–104, 44–56, 48, 36–38, 32–44, 30–36, 30–32, 200–204; AR 0.58–0.61. Palpomere length (μm): 40–44, 92–104, 140–164, 116–132, 164–180. Palpomere 3 in distal part with sensilla capitata with diameter 24 μm. Head width/palpal length 0.90–0.97. Antennal length/palpal length 0.88–0.97. Thorax. Antepronotum with 12–14 ventrolateral setae, 52–88 μm long. Dorsocentrals 20, 72–120 μm long; prealars 5, 52–116 μm long. Scutellum with ca 20 setae. Wing. Length 3.08–3.44 mm , width 0.96–1.0 mm. Anal lobe slightly reduced, angular. Squama with 24–26 setae (in 1–2 rows), 56–112 μm long. R and R 1 with 26–27 setae, R 4+5 with 5–6 (in distal half). Costal extension 76–82 μm. RM/MCu 2.5–3.0. Legs. Spur of front tibia 38–40 µm long. Spurs of mid tibia 40–44 µm and 40–48 µm. Spurs of hind tibia 60–68 µm and 40–44 µm long. Hind tibial comb with 21–25 setae. Length (μm) and proportions of leg segments are as in Table 10 . TABLE 10. Lengths (in µm) and proportions of leg segments of Diamesa moubayedi sp. nov. , male (n=5)
fe ti ta1 ta2 ta3 ta4 ta 5
P1 1920–2040 1840–1960 1200–1320 560–680 320–380 98–131 156–164
P 2 1720–1960 1520–1640 640–780 320–400 220–240 98–131 139–164
P3 1940–2100 1769–1960 1120–1300 600–660 340–360 123–131 164
continued
LR BV SV BR
P 1 0.64–0.70 3.78–4.41 3.0–3.17 1.1–1.2
P2 0.42–0.48 3.38–4.99 4.62–5.06 1.1
P3 0.64–0.66 3.93–4.14 3.12–3.30 1.1–1.2
Hypopygium ( Figs. 40, 43–44 , 46–47, 52, 55 ). Tergite IX densely covered with strong macrotrichia apices of which are directed anteriorly, with 7–15 setae, 32–48 µm long and with hitinized, narrow (12–14 µm) and naked anal point, 148–160 µm long, when viewed from side it is slightly curved in distal part ( Figs. 43–44 ). Laterosternite IX with 8–10 setae, 28–32 µm long. Transverse sternopodeme (TSA) triangular, 104–140 µm high, 148–184 µm wide at the base ( Fig. 52 ); TSA height/TSA width 0.80–0.89. Aedeagal lobe 164–176 µm long; phallapodeme sclerotized, 140–156 µm long. Gonocoxite 490–492 µm long; superior volsellae angular, along margin with some setae ( Fig. 55 ). Gonostylus 200–240 µm long, slightly curved, expanded in the distal half along the outer edge and with rounded–angular projection basally ( Fig. 47 ); in apical part with megaseta in form of wide terminal spine, 12 μm long and tooth the same size, next to it there is two setae approximately of the same length ( Fig. 46 ). HR 2.14–2.23. Pupa and larva unknown. Diagnosis. The male of D. moubayedi sp. nov. is closely related in the subgroup to D. zagrosica sp. nov and D. sakartvella but this species has longest body ( 3.7–4.1 mm ); transverse sternopodeme is triangular shape ( Fig. 52 ), of great height (104–140 µm) and not very wide basally (148–184 µm), the ratio of its height to width is 0.80–0.89; superior volsellae angular ( Fig. 55 ); gonostylus expanded in the distal half along the outer edge and with rounded– angular projection basally ( Fig.47 ). Total length of D. zagrosica sp. nov. 2.3–2.8 mm ; transverse sternopodeme is regular triangular shape ( Figs. 53–54 ), of medium height (84–108 µm) and not very wide basally (162–172 µm), the ratio of its height to width is 0.49–0.67; superior volsellae rounded–angular ( Fig. 56 ); gonostylus slightly expanded in the distal half along the outer edge and with small rounded projection basally ( Figs.48–49 ). Total length of D. sakartvella 2.9–3.1 mm ; t ransverse sternopodeme is wide triangular ( Figs. 50–51 ), of low height (68–88 µm) and very wide basally (172–188 µm), the ratio of its height to width is 0.36–0.50; superior volsellae rounded ( Fig. 57 ); gonostylus almost not expanded in the distal half along the outer edge and without projection basally ( Figs.45 ). Ecology. Adult males were collected in karstic springs and streams, located at an altitude of 2000 m . Water calcareous, conductivity 320–360 μS/cm, water temperature 8–10 °C during summer, about 4–8 °C in winter and spring (pers. comm. by J. Moubayed). Distribution. Known only from the type locality—karstic springs and streams of Sannyn Mount (Central Lebanon ) ( Fig. 75 ). Previously, this species, after the identification of B. Serra-Tosio (pers. comm. by J. Moubayed), was recorded for Lebanon as D. sakartvella Kownacki et Kownacka ( Moubayed & Laville 1983 ) .