Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of new species. I. Subgroups steinboecki and longipes Author Makarchenko, Eugenyi A. Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far East Branch of the Russian Academy of Sciences, 100 let Vladivostoku 159, 690022 Vladivostok, Russia Author Semenchenko, Alexander A. Laboratory of Ecology and Evolutionary Biology of Aquatic Organisms, Far Eastern Federal University, Suhanova St. 8, 690950 Vladivostok, Russia Author Palatov, Dmitry M. 0000-0002-8826-9316 A. N. Severtsov Institute of Ecology and Evolution of the Russian Academy of Sciences, Leninskij prosp. 33, 119071 Moscow, Russia triops @ yandex. ru; https: // orcid. org / 0000 - 0002 - 8826 - 9316 triops@yandex.ru text Zootaxa 2022 2022-04-13 5125 5 483 512 journal article 56018 10.11646/zootaxa.5125.5.2 6a677d3b-0f91-4f11-8cf2-64f4ad2a47d2 1175-5326 6457259 39EBA798-29B1-4450-9085-A4FB6D1DB331 Diamesa zagrosica Makarchenko et Semenchenko , sp. nov. http://zoobank.org/NomenclaturalActs/ 678CF541-8E78-4AC4-BE01-B73D857EA04E ( Figs. 37, 41 , 48–49, 53–54, 56 , 76 ) Type material. Holotype , adult male, IRAN : Zagros Mountains , Lorestan Province , Aligudarz County , Dalooni Preserve Park , Dare Daei River in the alpine zone of Zagros ridge, altitude 2244 m above sea level , N 33.186067 , E 49.510117 , 13.VI.2019 , leg. D. Palatov. Paratypes : 4 adult males, the same data as holotype ; 6 adult males, the same data as holotype, except Dez (Sezar) River , 1 km to North of Sepid Dasht Town , alt. 1022 m above sea level , N 33.226117 , E 48.885667 , 13.VI.2019 , leg. D. Palatov. Derivatio nominis. The species is named as zagrosica after the type locality in Zagros Mountains of Lorestan Province , Iran . Description Adult male (n = 5, except when otherwise stated). Total length 2.3–2.8 mm . Total length/wing length 0.89–0.90. Coloration. Dark brown to black. Head, thorax and abdomen dark brown. Legs light brown to brown. Wings dark grey, with brownish veins. Head ( Fig. 37 ). Eyes hairy, reniform. Temporal setae including 6–7 frontals, 10–12 orbitals, 7–13 verticals and 8–9 postorbitals. Clypeus with 9 setae. Antenna with 8 flagellomeres and reduced plume of setae; number and length of these setae on 1–7 flagellomeres respectively: 1–3 (32 μm), 3 (32–36 μm), 3 (32–36 μm), 0, 2 (48–52 μm), 0; terminal flagellomere with 6 setae, 72–88 μm long in basal part and with 2 subapical setae, 28–32 μm long. Length of 1–8 flagellomeres (μm): 80–96, 44–48, 40–44, 28–36, 28–32, 22–24, 22–24,160–164; AR 0.56–0.59. Palpomere length (μm): 44–52, 64–68, 96–100, 96–108, 136–140. Palpomere 3 in distal part with sensilla capitata with diameter 20–22 μm. Head width/palpal length 1.03–1.06. Antennal length/palpal length 0.94–0.97. Thorax. Antepronotum with 12–13 ventrolateral setae, 64–100 μm long. Dorsocentrals 17–20, 80–140 μm long (2 rows in front); prealars 7–10, 92–104 μm long. Scutellum with ca 20 setae. Wing. Length 2.56–2.68 mm , width 0.80–0.84 mm . Anal lobe rounded. Squama with 13–16 setae, 20–48 μm long. R and R 1 with 31–37 setae, R 4+5 with 6–9 (in distal part). RM/MCu 2.0–2.5. Legs. Spur of front tibia 28–36 µm long. Spurs of mid tibia 36–40 µm and 40 µm long. Spurs of hind tibia 68–74 µm and 44 µm long. Hind tibial comb with 17 setae. Length (μm) and proportions of leg segments are as in Table 8 . FIGURES 45–57. Some details of male’s hypopygium of Diamesa sakartvella Kownacki et Kownacka (45, 50–51, 57) , D. moubayedi sp. nov. (46–47, 52, 55) , D. zagrosica sp. nov. (48–49, 53–54, 56) . 45–49, gonostylus; 46, apex of gonostylus; 50–54, transverse sternapodeme; 55–57, superior volsellae. TABLE 8. Lengths (in µm) and proportions of leg segments of Diamesa zagrosica sp. nov. , male (n=5)

fe	ti	ta 1	ta 2	ta 3	ta 4	ta 5
P1	1427–1476	1312–1345	918–943	476	279–295	98–107		123–131
P2	1410–1423	1197–1214	374–590	287–295	197–210	98–107		115–131
P3	1492–1509	1394–1443	1017	508–525	295–312	98–107		123–131
continued
LR	BV	SV	BR
P 1	0.68–0.72		3.65–3.83	2.90–3.07	1.5–2.2
P2	0.47–0.49		4.43–4.59	4.42–4.57	1.1–1.2
P3	0.70–0.73		3.72–3.75	2.84–2.90	1.1–1.2

Hypopygium ( Figs. 41 , 48–49, 53–54, 56 ). Tergite IX densely covered with strong macrotrichia apices of which are directed anteriorly, with 11–13 setae, 12–22 µm long (laterally setae longer) and with narrow (8–12 µm), weakly chitinized and naked anal point, 120–140 µm long ( Fig. 41 ). Laterosternite IX with 9–16 setae, 24–28 µm long. Transverse sternopodeme (TSA) regular triangular, 84–108 µm high, 162–172 µm wide at the base ( Figs. 53–54 ); TSA height/TSA width 0.49–0.67. Aedeagal lobe 136–168 µm long; phallapodeme sclerotized, 108–112 µm long. Gonocoxite 352–380 µm long; superior volsellae rounded–angular, along margin with 4–6 setae, 28–36 µm long ( Fig. 56 ). Gonostylus 148–168 µm long, weakly curved, slightly expanded in the distal half along the outer edge ( Figs. 48–49 ); in apical part with megaseta in form of wide terminal spine, 8–12 μm long and tooth the same size, next to it there is two setae approximately of the same length. HR 2.15–2.26. Pupa and larva unknown. Diagnosis. The male of D. zagrosica sp. nov. is closely related in the subgroup to the D. sakartvella and D. moubayedi sp. nov. from which it can be distinguished by shape and structure of transverse sternopodeme, inferior volsellae and gonostylus. Transverse sternopodeme of D. zagrosica sp. nov. is regular triangular shape ( Figs. 53–54 ), of medium height (84–108 µm) and not very wide basally (162–172 µm), the ratio of its height to width is 0.49–0.67; superior volsellae rounded–angular ( Fig. 56 ); gonostylus slightly expanded in the distal half along the outer edge and with small rounded projection basally ( Figs.48–49 ). Also, male of this species has shortest legs, femur of front leg 1427–1476 µm long. Transverse sternopodeme of D. sakartvella sp. nov. is wide triangular ( Figs. 50–51 ), of low height (68–88 µm) and very wide basally (172–188 µm), the ratio of its height to width is 0.36–0.50; superior volsellae rounded ( Fig. 57 ); gonostylus almost not expanded in the distal half along the outer edge and without projection basally ( Figs.45 ); femur of front leg 1607–1960 µm long. Transverse sternopodeme of D. moubayedi sp. nov. is triangular shape ( Fig. 52 ), of great height (104–140 µm) and not very wide basally (148–184 µm), the ratio of its height to width is 0.80–0.89; superior volsellae angular ( Fig. 55 ); gonostylus expanded in the distal half along the outer edge and with rounded–angular projection basally ( Figs. 40 , 47 ); femur of front leg 1920–2040 µm long. Ecology. Adult males were collected from stones and boulders in high mountain river, located at an altitude of 2244 m , at a flow rate of 0.4–0.9 m /s, with water temperatures ca 4.8°C. Distribution. Known only from the type locality—Zagros Mountains in Iran ( Fig. 76 ).