Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of new species. I. Subgroups steinboecki and longipes Author Makarchenko, Eugenyi A. Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far East Branch of the Russian Academy of Sciences, 100 let Vladivostoku 159, 690022 Vladivostok, Russia Author Semenchenko, Alexander A. Laboratory of Ecology and Evolutionary Biology of Aquatic Organisms, Far Eastern Federal University, Suhanova St. 8, 690950 Vladivostok, Russia Author Palatov, Dmitry M. 0000-0002-8826-9316 A. N. Severtsov Institute of Ecology and Evolution of the Russian Academy of Sciences, Leninskij prosp. 33, 119071 Moscow, Russia triops @ yandex. ru; https: // orcid. org / 0000 - 0002 - 8826 - 9316 triops@yandex.ru text Zootaxa 2022 2022-04-13 5125 5 483 512 journal article 56018 10.11646/zootaxa.5125.5.2 6a677d3b-0f91-4f11-8cf2-64f4ad2a47d2 1175-5326 6457259 39EBA798-29B1-4450-9085-A4FB6D1DB331 Diamesa dragani Makarchenko et Semenchenko , sp. nov. http://zoobank.org/NomenclaturalActs/ B3C5EFC4-7AE3-4419-B2AF-DD40873B467B ( Figs. 1–8 , 30 , 68 ) Type material. Holotype , adult male, RUSSIA : Republic of Khakassia , Tashtypskyi District , Sayan Mountains , unnamed stream of Bolshoi On River basin, altitude 2063 m above sea level , 4.X.2020 , N 51.714667 , E 89.884500 , leg. S. Dragan. Paratypes : 6 adult males, the same data as holotype . Derivatio nominis. The species is named in honor of Dr. Dragan Sergey Viktorovich from Katanov Khakas State University (Abakan, Republic of Khakassia , Russia ) who collected type material in Sayan Mountains. Description Adult male (n = 7, except when otherwise stated). Total length 2.1–2.5 mm . Total length/wing length 0.81–1.20. Coloration. Dark brown to black. Head, thorax and abdomen dark brown to black. Legs black. Wings grey, with brownish veins. Head ( Figs. 1–2 ). Eyes hairy, reniform. Temporal setae including 6–7 frontals, 3–6 orbitals, 5–10 verticals and 3–4 postorbitals. Clypeus with 4–6 setae.Antenna with 7 flagellomeres and reduced plume of setae ( Fig. 2 ); number and length of these setae on 1–6 flagellomeres respectively: 1–2 (26–28 μm), 1–2 (18–32 μm), 3 (32–44 μm), 1–2 (24–32 μm), 2 (40–44 μm), 4 (40–48 μm); terminal flagellomere with 2–4 setae, 32–48 μm long in basal part and with 2 subapical setae, 20–24 μm long. Length of 1–7 flagellomeres (μm): 96–100, 40–48, 32–36, 24–28, 24–28, 20–24, 120–128; AR 0.48–0.52. Palpomere length (μm): 28–40, 68–72, 84–92, 64–68, 88–100. Palpomere 3 in distal part with sensilla capitata with diameter 12–15 μm. Head width/palpal length 1.0–1.29. Antennal length/palpal length 1.07–1.09. Thorax. Antepronotum with 8–11 ventrolateral setae, 44–52 μm long. Dorsocentrals 4–6, 56–72 μm long; prealars 5–6, 48–60 μm long. Scutellum with 18–20 setae. AAII with 4–5 setae, 36 μm long. Wing ( Fig. 30 ). Length 2.16–2.72 mm , width 0.85–0.88 mm . Costal extension 60–76 µm long. Anal lobe rounded-angular. Squama with 3–7 setae, 32–44 μm long. R and R 1 with 7–12 setae, R 4+5 with 13–15. RM/MCu 1.8–2.0. Legs. Spur of front tibia 36–40 µm long. Spurs of mid tibia 36–44 µm and 40–44 µm long. Spurs of hind tibia 64–72 µm and 40–44 µm long. Hind tibial comb with 16–20 setae. Length (μm) and proportions of leg segments are as in Table 1 . FIGURES 1–8. Diamesa dragani sp. nov. , male. 1, head; 2, antenna; 3, tergite IX; 4, hypopygium in dorsal view; 5, gonocoxite and gonostylus; 6, apex of gonostylus; 7, anal point in lateral view; 8, transverse sternapodeme, phallapodeme and aedeagal lobe. ATB—anterolateral anal tergal bands, MTB—median anal tergal band, PTB—posterolateral anal tergal band. TABLE 1. Lengths (in µm) and proportions of leg segments of Diamesa dragani sp. nov. , male (n=5)

fe	ti	ta1	ta2	ta3	ta4	ta 5
P1	1394–1689	1230–1394	738–787	312–344	180–197	82–98	115–131
P 2	1279–1542	984–1132	410–459	197–213	131–148	66–98	98–115
P3	1394–1689	1214–1410	754–787	402–443	213–230	98	115–131
continued
LR	BV	SV	BR
P 1	0.56–0.60	4.46–5.25	3.56–3.92	1.5–1.7
P2	0.40–0.43	4.96–5.60	5.31–5.83	1.5–1.7
P3	0.53–0.62	3.87–4.29	3.49–4.11	1.7–2.0

Hypopygium ( Figs. 3–8 ). Tergite IX with 8–14 setae, 16–24 µm long and with narrow (4–6 µm), pale, and naked anal point, 120–126 µm long which in lateral view apically curved upwards ( Fig. 7 ); posterior edge of tergite IX almost straight, anal-lateral angles are rounded and slightly projecting ( Fig. 3 ); anal tergal bands X-type; median tergal band 16–20 µm long. Laterosternite IX with 7–12 setae, 20–24 µm long. Transverse sternopodeme (TSA) triangular, peaked ( Figs. 4, 8 ), 120–128 µm high, 156–176 µm wide at the base; TSA height/TSA width 0.70–0.77. Aedeagal lobe as in Fig. 8 , weakly chitinized, apically with small digitiform process; phallapodeme sclerotized, 210–232 µm long. Gonocoxite 400–443 µm long, in middle part of dorso-internal position with small flattened transverse lobe, 98–100 µm long and 28–32 µm wide, directed backwards, and with large longitudinal lobe in middle, anterior outer margin of which is rounded or rounded-angular and slightly projecting ( Fig. 5 ); Gonostylus 197–236 µm long, slightly curved, megaseta in form of wide terminal spine 8–12 μm long, next to it there is tooth and two setae of 12–16 length µm long ( Fig. 6 ). HR 2.01–2.25. Pupa and larva unknown. Diagnosis. The male of D. dragani sp. nov. is most closely related in the subgroup to the D. maisaraensis sp. nov. and D. marinskiyi sp. nov. , which have anal tergal bands X-type, but the new species is a little shorter than others ( 2.1–2.5 mm ), antenna with 7 flagellomeres, AR 0.48–0.52, dorsocentrals 4–6, scutellars 18–20, femur of front leg 1394–1689 µm long, median tergal band 16–20 µm long, anal point 230–126 µm long. The males of D. maisaraensis sp. nov. and D. marinskiyi sp. nov. have the following characteristics respectively: total length 2.7–3.2 mm and 2.5–2.7 mm , antenna with 8 flagellomeres, AR 0.39–0.42 and 0.32–0.39, dorsocentrals 6–8 and 8–10, scutellars 39–50 and 44–46, femur of front leg length 1880–1960 µm and 1624–1476 µm, median tergal band length 28–32 µm and 20–24 µm, anal point length 100–120 µm and 104–116 µm. Ecology. Adult males were collected from stones and boulders in mountain stream, located at an altitude of 2063 m , at a flow rate of 0.6 m /s, with water temperatures 0.4°C, pH 7.92, general mineralization 0.02 g /l, electrical conductivity 0.03 mS (pers. comm. by S.V. Dragan). Distribution. Known only from the type locality in Sayan Mountains ( Fig. 68 ).