A species-level taxonomic review and host associations of Glyptapanteles (Hymenoptera, Braconidae, Microgastrinae) with an emphasis on 136 new reared species from Costa Rica and Ecuador Author Arias-Penna, Diana Carolina Author Whitfield, James B. Author Janzen, Daniel H. Author Winifred Hallwachs, Author Dyer, Lee A. Author Smith, M. Alex Author Hebert, Paul D. N. Author Fernandez-Triana, Jose L. text ZooKeys 2019 890 1 685 http://dx.doi.org/10.3897/zookeys.890.35786 journal article http://dx.doi.org/10.3897/zookeys.890.35786 1313-2970-890-1 FD8F695311F64DF2950F6A387340BCE5 2691DADB7BA352BEBA377C901FC0AC97 Glyptapanteles shelbystedenfeldae Arias-Penna, sp. nov. Figs 201 , 202 Male. Body length 2.17 mm , antenna length 3.08 mm , fore wing length 3.68 mm . Type material. Holotype : COSTA RICA • 1♀ ; 00-SRNP-24000, DHJPAR0013608; Area de Conservacion Guanacaste , Guanacaste , Sector Santa Rosa , Bosque Humedo ; dry forest; Malasie ; 290 m ; 10.85145 , -85.60801 ; 24.iv.2000 ; DH Janzen & W Hallwachs leg.; ( CNC ) . Paratypes . • 1 (0 ♀ , 0 ♂ ) (0 ♀ , 1♂ ); 00-SRNP-23957, DHJPAR0013596; same data as for holotype except: 10.iv.2000 ; ( CNC ) . Other material. Malaise-trapped material. COSTA RICA : Area de Conservacion Guanacaste , Guanacaste , Sector Santa Rosa , Bosque Humedo : • 1 ( 1♀ , 0 ♂ ) (0 ♀ , 0 ♂ ); 98-SRNP-16106, DHJPAR0013373; Malaise ; dry forest; 290 m ; 10.85145 , -85.60801 ; 09.iii.1998 ; DH Janzen & W Hallwachs leg. • 1 (0 ♀ , 1♂ ) (0 ♀ , 0 ♂ ); 98-SRNP-16130, DHJPAR0013579; same data as for preceding except: 30.iii.1998 . • 1 (0 ♀ , 0 ♂ ) (0 ♀ , 1♂ ); 99-SRNP-19079, DHJPAR0013581; same data as for preceding except: 03.v.1999 . • 1 (0 ♀ , 1♂ ) (0 ♀ , 0 ♂ ); 99-SRNP-19086, DHJPAR0013583; same data as for preceding except: 03.v.1999 . • 1 (0 ♀ , 1♂ ) (0 ♀ , 0 ♂ ); 99-SRNP-19096, DHJPAR0013587; same data as for preceding except: 10.v.1999 . • 1 (0 ♀ , 1♂ ) (0 ♀ , 0 ♂ ); 00-SRNP-24025, DHJPAR0013408; same data as for preceding except: 06.iii.2000 . • 1 (0 ♀ , 0 ♂ ) (0 ♀ , 1♂ ); 00-SRNP-24006, DHJPAR0013414; same data as for preceding except: 27.iii.2000 . • 1 (0 ♀ , 1♂ ) (0 ♀ , 0 ♂ ); 00-SRNP-23961, DHJPAR0013599; same data as for preceding except: 22.v.2000 . • 1 (0 ♀ , 1♂ ) (0 ♀ , 1♂ ); 00-SRNP-23988, DHJPAR0013607; same data as for preceding except: 22.v.2000 . • 1 (0 ♀ , 1♂ ) (0 ♀ , 0 ♂ ); 07-SRNP-15057, DHJPAR0013617; same data as for preceding except: 05.vi.2007 ; AR Deans & J Rodriguez leg. Area de Conservacion Guanacaste, Guanacaste , Sector Santa Rosa , Bosque San Emilio : • 1 (0 ♀ , 1♂ ) (0 ♀ , 0 ♂ ); 00-SRNP-23869, DHJPAR0013555; Malaise; dry forest; 300 m ; 10.84389 ; -85.61384 ; 22.v.2000 ; DH Janzen & W Hallwachs leg. Diagnosis. Precoxal groove with transverse lineate sculpture ( Figs 201A, I , 202E ), medioanterior pit of metanotum circular and bisected by a median longitudinal carina ( Figs 201F , 202C ), inner margin of eyes diverging slightly at antennal sockets ( Fig. 201B ), scutellar punctation distinct throughout ( Figs 201E, F , 202B, C ), fore wing with 1 cu-a vein complete, but junction with 1-1A vein spectral, outer side of junction of r and 2RS veins not forming a stub ( Fig. 202D ), propodeum with a clearly visible median longitudinal carina ( Figs 201F , 202C ), and lateral grooves delimiting the median area on T2 clearly defined and reaching the distal edge of T2 ( Figs 201G, H ). Figure 201. Glyptapanteles shelbystedenfeldae sp. nov. female 98-SRNP-16106 DHJPAR0013373 A Habitus B, D Head B Frontal view D Dorsal view C Head, pronotum, propleuron, lateral view E Mesonotum, dorsal view F Scutellum, metanotum, propodeum, dorsal view G T1-3, dorsal view H, J Metasoma H Dorsal view J Lateral view I Mesosoma, lateral view. Figure 202. Glyptapanteles shelbystedenfeldae sp. nov. male 00-SRNP-23869 DHJPAR0013555, 00-SRNP-24020 DHJPAR0013403 A Habitus B Mesonotum, dorsal view C Scutellum, metanotum, propodeum, dorsal view D Fore and hind wings E Mesosoma, lateral view F Metasoma, lateral view. Coloration ( Fig. 202 A-F ). General body coloration black except scape yellow-brown/reddish distally with a brown band; pedicel yellow-brown/reddish; first four-five proximal antennal flagellomeres dorsally lighter (light brown) than ventrally (dark brown), remaining flagellomeres dark brown on both sides; labrum, mandible, and tegulae yellow; propleuron distally with a tiny dot, dorsal furrow of pronotum, and epicnemial ridge with yellow-brown/reddish tints; glossa, maxillary and labial palps pale yellow/ivory. Eyes and ocelli silver. Fore and middle legs dark yellow except brown claws; hind legs dark yellow except black coxae, femora distally brown, and tibiae with both ends brown. Petiole on T1 light brown, contours darkened and sublateral areas ivory/pale yellow; T2 with median and adjacent areas brown, and lateral ends ivory/pale yellow; T3 brown, but 1/3 proximal of lateral ends ivory/pale yellow; T4 and beyond completely brown; distally each tergum with a narrow yellowish translucent band. In lateral view, T1-3 ivory/pale yellow; T4 and beyond yellow with brown tints. S1-2 ivory/pale yellow; S3 yellow, but medially brown; S4 and beyond brown. Description. Head ( Fig. 202A ). Head rounded with pubescence long and dense. Proximal three antennal flagellomeres longer than wide (0.19:0.07, 0.20:0.07, 0.19:0.07), distal antennal flagellomere longer than penultimate (0.13:0.05, 0.10:0.05), antenna longer than body (3.08, 2.17); antennal scrobes-frons shallow. Face convex with dense fine punctations, interspaces wavy and longitudinal median carina present. Frons smooth. Temple wide, punctate-lacunose and interspaces wavy. Inner margin of eyes diverging slightly at antennal sockets; in lateral view, eye anteriorly convex and posteriorly straight. POL subequal in length with OOL (0.09, 0.10). Malar suture present. Median area between lateral ocelli slightly depressed. Vertex laterally pointed or nearly so and dorsally wide. Mesosoma ( Fig. 202 A-C , E ). Mesosoma dorsoventrally convex. Mesoscutum proximally convex and distally flat, punctation distinct throughout, interspaces wavy/lacunose. Scutellum triangular, apex sloped and fused with BS , scutellar punctation distinct throughout, in profile scutellum flat and on same plane as mesoscutum, phragma of the scutellum partially exposed; BS not overlapping the MPM ; ATS demilune with a little, complete and parallel carinae; dorsal ATS groove with semicircular/parallel carinae. Transscutal articulation with small and heterogeneous foveae, area just behind transscutal articulation smooth, shiny and depressed centrally. Metanotum with BM wider than PFM (clearly differentiated); MPM circular and bisected by a median longitudinal carina; AFM with a small lobe and not as well delineated as PFM ; PFM thick, smooth and with lateral ends rounded; ATM proximally with a groove with some sculpturing and distally smooth. Propodeum with a clearly visible median longitudinal carina, proximal half curved rather coarse sculpture and distal half rugose; distal edge of propodeum with a flange at each side and without stubs; propodeal spiracle distally framed by a short concave carina; nucha surrounded by very short radiating carinae. Pronotum with a distinct dorsal furrow, dorsally with a well-defined smooth band; both dorsal and ventral furrows and central area of pronotum with sculpture. Propleuron rugose and dorsally with a carina. Metasternum convex. Contour of mesopleuron convex; precoxal groove deep with transverse lineate sculpture; epicnemial ridge convex, teardrop-shaped. Legs ( Fig. 202A, F ). Ventral margin of fore telotarsus entire without seta, fore telotarsus almost same width throughout and longer than fourth tarsomere (0.10, 0.06). Medially hind coxa smooth, dorsally with scattered punctation and ventrally with dense punctation, dorsal outer depression present. Inner spur of hind tibia longer than outer spur (0.23, 0.17), entire surface of hind tibia with dense strong spines clearly differentiated by color and length. Hind telotarsus as equal in length as fourth tarsomere (0.11, 0.11). Wings ( Fig. 202D ). Fore wing with r vein curved; 2RS vein slightly concave; r and 2RS veins forming a weak, even curve at their junction and outer side of junction not forming a stub; 2M vein slightly curved/swollen; distally fore wing [where spectral veins are] with microtrichiae more densely concentrated than the rest of the wing; anal cell 1/3 proximally lacking microtrichiae; subbasal cell with a small smooth area; vein 2CUa absent and vein 2CUb spectral; vein 2 cu-a absent; vein 2-1A proximally tubular and distally spectral, although sometimes difficult to see; tubular vein 1 cu-a curved and complete, but junction with 1-1A vein spectral. Hind wing with vannal lobe narrow, subdistally and subproximally straightened, and setae evenly scattered in the margin. Metasoma ( Fig. 202A, F ). Metasoma laterally compressed. Petiole on T1 finely sculptured only distally, evenly narrowing distally (length 0.31, maximum width 0.15, minimum width 0.04) and with scattered pubescence concentrated in the first distal third. Lateral grooves delimiting the median area on T2 clearly defined and reaching the distal edge of T2 (length median area 0.11, length T2 0.11), edges of median area polished and lateral grooves deep, median area broader than long (length 0.11, maximum width 0.14, minimum width 0.04); T2 with scattered pubescence only distally. T3 longer than T2 (0.19, 0.11) and with scattered pubescence throughout. Pubescence on hypopygium dense. Cocoons. Unknown. Comments. The sculpture on body are dense ( Fig. 201A, F ), the hind coxae is stout, and both sexes with stout and short bodies. Female ( Fig. 201 A-J ). The metasoma is missing in the only preserved female; however, there is a photographic record. In female, the distal corners of mesoscutum are yellow-brown/reddish. Etymology. Shelby E. Stedenfeld as graduate student at the Kentucky University, Lexington, KY, USA , was interested in the taxonomy and systematics of Ichneumonidae and their roles in biological control. Distribution. The adult parasitoids were collected in Costa Rica , ACG, Sector Santa Rosa (Bosque Humedo and Bosque San Emilio), during May 1999 , March-May 2000, and June 2007 at 290 m in dry forest. Biology. Unknown. Host. Unknown.