Minimalist revision and description of 403 new species in 11 subfamilies of Costa Rican braconid parasitoid wasps, including host records for 219 species Author Sharkey, Michael J. https://orcid.org/0000-0001-6201-7340 The Hymenoptera Institute, 116 Franklin Ave., Redlands, CA, 92373, USA msharkey@uky.edu Author Janzen, Daniel H. Department of Biology, University of Pennsylvania, Philadelphia, PA 19104 - 6018, USA Author Hallwachs, Winnie Department of Biology, University of Pennsylvania, Philadelphia, PA 19104 - 6018, USA Author Chapman, Eric G. Department of Entomology, University of Kentucky, Lexington, KY 40546 - 0091, USA Author Smith, M. Alex https://orcid.org/0000-0002-8650-2575 Department of Integrative Biology, University of Guelph and Biodiversity Institute of Ontario, Guelph, Canada Author Dapkey, Tanya Academy of Natural Sciences, 1900 Benjamin Franklin Parkway, Philadelphia, PA 19103, USA Author Brown, Allison Academy of Natural Sciences, 1900 Benjamin Franklin Parkway, Philadelphia, PA 19103, USA Author Ratnasingham, Sujeevan Academy of Natural Sciences, 1900 Benjamin Franklin Parkway, Philadelphia, PA 19103, USA Author Naik, Suresh Academy of Natural Sciences, 1900 Benjamin Franklin Parkway, Philadelphia, PA 19103, USA Author Manjunath, Ramya Academy of Natural Sciences, 1900 Benjamin Franklin Parkway, Philadelphia, PA 19103, USA Author Perez, Kate Academy of Natural Sciences, 1900 Benjamin Franklin Parkway, Philadelphia, PA 19103, USA Author Milton, Megan Academy of Natural Sciences, 1900 Benjamin Franklin Parkway, Philadelphia, PA 19103, USA Author Hebert, Paul Academy of Natural Sciences, 1900 Benjamin Franklin Parkway, Philadelphia, PA 19103, USA Author Shaw, Scott R. Department of Ecosystem Science, University of Wyoming, 1000 East University Avenue, Laramie, Wyoming 82071, USA Author Kittel, Rebecca N. https://orcid.org/0000-0003-0032-5764 Museum Wiesbaden, Hessisches Landesmuseum fuer Kunst und Natur, Friedrich-Ebert-Allee 2, 65185 Wiesbaden, Germany Author Solis, M. Alma https://orcid.org/0000-0001-6379-1004 Systematic Entomology Laboratory, Beltsville Agriculture Research Center, Agricultural Research Service, U. S. Department of Agriculture, c / o National Museum Natural History, MRC 168, Smithsonian Institution, P. O. Box 37012, Washington, DC, 20013 - 7012, USA Author Metz, Mark A. Systematic Entomology Laboratory, Beltsville Agriculture Research Center, Agricultural Research Service, U. S. Department of Agriculture, c / o National Museum Natural History, MRC 168, Smithsonian Institution, P. O. Box 37012, Washington, DC, 20013 - 7012, USA Author Goldstein, Paul Z. Systematic Entomology Laboratory, Beltsville Agriculture Research Center, Agricultural Research Service, U. S. Department of Agriculture, c / o National Museum Natural History, MRC 168, Smithsonian Institution, P. O. Box 37012, Washington, DC, 20013 - 7012, USA Author Brown, John W. Division of Entomology, PO Box 37012 12. National Museum of Natural History E 515 MRC 127, Washington, DC 20013 - 7012, USA Author Quicke, Donald L. J. Department of Biology, Faculty of Life Sciences, Chulalongkorn University, Bangkok, Thailand Author Achterberg, C. van https://orcid.org/0000-0002-6495-4853 Naturalis Biodiversity Center, Postbus 9517, 2300 RA Leiden, The Netherlands Author Brown, Brian V. https://orcid.org/0000-0001-6367-6057 Department of Entomology, Natural History Museum of Los Angeles County, 900 Exposition Boulevard, Los Angeles, CA, 90007, USA Author Burns, John M. Division of Entomology, PO Box 37012 12. National Museum of Natural History E 515 MRC 127, Washington, DC 20013 - 7012, USA text ZooKeys 2021 2021-02-02 1013 1 665 http://dx.doi.org/10.3897/zookeys.1013.55600 journal article http://dx.doi.org/10.3897/zookeys.1013.55600 1313-2970-1013-1 CFDCEFBB523040339D46E302F66E9886 E4329863A39E5EEBA395938413BDD579 Bracon tihisiaboshartae Sharkey sp. nov. Figures 72 , 73 Diagnostics. BOLD:AAA5367. Consensus barcode. TGTATTATATTTTTTATTTGGAATATGAGCYGGAATAATTGGTTTATCAATAAGTTTAATTATTCGTTTAGAATTAGGRATACCAGGTAGTTTAYTAGGTAATGATCAAATTTATAATAGTATAGTTACAGCKCATGCTTTTATTATAATTTTTTTTATAGTTATACCAGTAATATTAGGAGGWTTTGGTAATTGATTAGTTCCTTTAATATTAGGTGCTCCTGATATAGCTTTYCCTCGAATAAATAATATAAGATTTTGATTATTAATTCCTTCATTAATTTTATTATTATTAAGAAGAATTTTAAATGTTGGTGTAGGRACAGGCTGAACTATTTATCCTCCTTTATCTTCTATAATAGGTCATAGAGGWATATCTGTRGATTTATCTATTTTYTCTTTACATTTAGCTGGTATTTCTTCTATTATAGGATCGATTAATTTTATTACAACAATTTTAAATATACATTTATTAATATTAAAATTAGATCAATTAACTTTATTTATTTGATCAATTTTTATTACAACTATTTTATTATTATTATCTTTACCTGTATTAGCAGGAGCTATTACTATAYTATTAACTGATCGAAATTTWAATACTTCATTTTTTGATTTTTCTGGAGGTGGGGATCCAATTYTATTTCAACATTTATTT. Bracon tihisiaboshartae and B. alejandromasisi occupy the same BIN. Bracon tihisiaboshartae can be differentiated from B. alejandromasisi by the color of the metasomal terga: entirely yellow in B. tihisiaboshartae and partly black in B. alejandromasisi . The two can also be differentiated based on their host caterpillars and style of cocoons, see note below. Holotype ♀. Guanacaste, Sector Mundo Nuevo, Quebrada Tibio Perla, 10.76261 , -85.42979 , 330 meters, caterpillar collection date: 17/xi/2013, wasp eclosion date: 1/xii/2013, 26 wasps eclosed. Depository: CNC. Host data . Gregarious parasitoid of Memphis boisduvali ( Nymphalidae ) feeding on Mespilodaphne veraguensis ( Lauraceae ) (Fig. 72 ) Caterpillar and holotype voucher codes . 13-SRNP-57185, DHJPAR0054608. Paratype. Host = Memphis pithyusa : DHJPAR0040072. Depository: CNC. Note. Bracon alejandromasisi and Bracon tihisiaboshartae are placed in the same BIN, but their COI barcodes differ by ca. 1%, and each is reared from different genera of caterpillars ( Consul , Memphis ) feeding on very different plants ( Piper , Croton ). The cocoons of the former are dark brown and tightly fill the caterpillar nest (Fig. 32 ), whereas the cocoons of the later are white-golden and form an irregular mass in a much looser leaf roll nest of the host caterpillar, not filling it (Fig. 73 ). Experience has shown that with this level of difference within ACG ecosystems and hosts, larger sample sizes demonstrate that this species-level taxonomic hypothesis is almost certainly correct. Etymology. Bracon tihisiaboshartae is name in honor of Tihisia Boshart in specific recognition of her rapid and high-quality rendering of artwork for Hymenoptera and Lepidoptera patronyms for major Costa Rican decision-makers helping ACG. Figure 72. Bracon tihisiaboshartae , holotype. Figure 73. Bracon tihisiaboshartae (09-SRNP-56390-DHJ474143.jpg) white-golden cocoons piled together in two masses on the leaf, rather than filling the nest cavity as the case with Bracon alejandromasisi ; wasp exit hole is in the mass on the left.