Two new species of Botryosphaeriaceae (Botryosphaeriales) and new host / geographical records Author Rathnayaka, Achala R. 0000-0001-8498-2715 School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand. & Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand. & Department of Plant Medicine, National Chiayi University, 300 Syuefu Road, Chiayi City 60004, Taiwan. & rathnayakaachala @ gmail. com; https: // orcid. org / 0000 - 0001 - 8498 - 2715 rathnayakaachala@gmail.com Author Chethana, K. W. Thilini 0000-0002-5816-9269 School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand. & Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand. & tchethi @ yahoo. com; https: // orcid. org / 0000 - 0002 - 5816 - 9269 tchethi@yahoo.com Author Phillips, Alan J. L. 0000-0001-6367-9784 Faculdade de Ciências, Biosystems and Integrative Sciences Institute (BioISI), Universidade de Lisboa, Campo Grande, 1749 - 016 Lisbon, Portugal. & alan. jl. phillips @ gmail. com; https: // orcid. org / 0000 - 0001 - 6367 - 9784 alan.jl.phillips@gmail.com Author Jones, E. B. Gareth 0000-0002-7286-5471 Department of Botany and Microbiology, College of Science, King Saud University, P. O Box 2455, Riyadh 11451, Kingdom of Saudi Arabia. & torperadgj @ gmail. com; https: // orcid. org / 0000 - 0002 - 7286 - 5471 torperadgj@gmail.com text Phytotaxa 2022 2022-09-14 564 1 8 38 journal article 142529 10.11646/phytotaxa.564.1.2 4b4efc13-1b6e-4b55-a5d7-397f80b46b4c 1179-3163 7077775 Neodeightonia phoenicum A.J.L. Phillips & Crous , in Phillips et al. , Persoonia 21: 43 (2008) Index Fungorum number : IF 511708; Facesoffungi number : FoF 11670, Fig. 13 Saprobic on dead leaf base of Phoenix reclinata . Sexual morph : Undetermined. Asexual morph: Coelomycetous. Conidiomata 90–150 μm high × 75–100 μm diam. ( x̄ = 120 × 88 μm, n = 10), pycnidial, solitary or gregarious, subglobose, multiloculate, dark brown to black, immersed, becoming erumpent at maturity. Conidiomata wall 10–30 μm wide, two-layered, outer layer composed of thick-walled, dark brown cells of textura angularis , inner layer composed of thin-walled, pale brown to hyaline cells of textura angularis . Conidiophores usually reduced to conidiogenous cells. Conidiogenous cells 5–10 × 2–5 μm ( x̄ = 7.4 × 3 μm, n = 20), lining the pycnidial cavity, holoblastic, hyaline, cylindrical, discrete, determinate, smooth-walled. Conidia 11–17 × 6–10 μm ( x̄ = 14 × 8.5 μm, n = 20), ovoid to ellipsoid, straight, broadly rounded at both ends, hyaline, aseptate, guttulate. FIGURE 12. Neodeightonia arengae on dead branch of Arenga tremula (NCYUCC 19-0419). a−c. Appearance of ascomata on host surface. d, e. Section of ascoma. f. Peridium . g. Pseudoparaphyses. h−k. Asci. l−n. Ascospores with a wing-like appendages (in water). o. Ascospore in 100% lactic acid. p, q. Colony on PDA (p upper, q lower). Scale bars: a = 1 mm, b = 500 μm, c–e = 100 μm, f = 5 μm, g–k = 20 μm, l–o = 10 μm. FIGURE 13. Neodeightonia phoenicum on dead leaf base of Phoenix reclinata (MFLU 22-0096). a, b. Host. c, d. Appearance of conidiomata on host surface. e. Section through conidiomata. f. Section through peridium. g–j. Conidiogenous cells. k−n. Conidia . o. Germinating conidium. p, q. Colony on PDA (p upper, q lower). Scale bars: c = 2 mm, d = 100 μm, e = 50 μm, f, g = 5 μm, h–o = 10 μm. Culture characteristics: — Conidia germinating on PDA within 24 h. Germ tubes produced from one side of the conidium. Colonies on PDA reaching 5–6.5 cm diam. after 4 days at 25 °C, circular, flattened, fluffy, medium dense, aerial, grey in top view and black in reverse. Material examined :— THAILAND . Chiang Rai : near Public at Rajabath university, on a dead leaf base of Phoenix reclinata ( Arecaceae ) , 12 June 2020 , Achala Rathnayaka (MFLU 22-0096, new host and geographical record), living culture MFLUCC 22-0061. Known hosts and distribution :— Phoenix canariensis in Spain ( Phillips et al. 2008 , Liu et al. 2010), P. dactylifera in California and Spain ( Phillips et al. 2008 , Liu et al. 2010), Phoenix sp. in Greece ( Ligoxigakis et al. 2013 ), and P. reclinata in Thailand (this study). Notes :—Morphologically, our collection (MFLUCC 22-0061) is similar to the holotype of Neodeightonia phoenicum (CBS 122528) collected from Phoenix sp. in Spain ( Phillips et al. 2008 ). Both collections have dark brown to black, multiloculate, erumpent conidiomata. Conidiogenous cells are holoblastic, hyaline, cylindrical, and conidia are ovoid to ellipsoid. However, the size of the conidia of our new collection ( x̄ = 14 × 8.5 μm) is smaller than the extype strain ( x̄ = ± S.D. = 19.1 ± 1.7 × 11.5 ± 1.1 μm) ( Phillips et al. 2008 ). In the multi-gene phylogeny (ITS, LSU, SSU, and tef 1-α), our strain together with the ex-type and other strains of N. phoenicum (CBS 122528, CBS 123168 and CBS 169.34) clustered with relatively high 100% ML bootstrap and 1.00 PP support ( Fig. 3 ). Based on morphmolecular analyses, we introduce our collection as a new host record of N. phoenicum from Phoenix reclinata and also as a new geographical record from Thailand .