Two new species of Botryosphaeriaceae (Botryosphaeriales) and new host / geographical records Author Rathnayaka, Achala R. 0000-0001-8498-2715 School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand. & Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand. & Department of Plant Medicine, National Chiayi University, 300 Syuefu Road, Chiayi City 60004, Taiwan. & rathnayakaachala @ gmail. com; https: // orcid. org / 0000 - 0001 - 8498 - 2715 rathnayakaachala@gmail.com Author Chethana, K. W. Thilini 0000-0002-5816-9269 School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand. & Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand. & tchethi @ yahoo. com; https: // orcid. org / 0000 - 0002 - 5816 - 9269 tchethi@yahoo.com Author Phillips, Alan J. L. 0000-0001-6367-9784 Faculdade de Ciências, Biosystems and Integrative Sciences Institute (BioISI), Universidade de Lisboa, Campo Grande, 1749 - 016 Lisbon, Portugal. & alan. jl. phillips @ gmail. com; https: // orcid. org / 0000 - 0001 - 6367 - 9784 alan.jl.phillips@gmail.com Author Jones, E. B. Gareth 0000-0002-7286-5471 Department of Botany and Microbiology, College of Science, King Saud University, P. O Box 2455, Riyadh 11451, Kingdom of Saudi Arabia. & torperadgj @ gmail. com; https: // orcid. org / 0000 - 0002 - 7286 - 5471 torperadgj@gmail.com text Phytotaxa 2022 2022-09-14 564 1 8 38 journal article 142529 10.11646/phytotaxa.564.1.2 4b4efc13-1b6e-4b55-a5d7-397f80b46b4c 1179-3163 7077775 Sphaeropsis eucalypticola (Doilom, J.K. Liu, & K.D. Hyde) A.J.L. Phillips , in Phillips et al. , Stud. Mycol. 76: 158 (2013) Synonym: Sphaeropsis chromolaenicola Mapook & K.D. Hyde , in Mapook et al. , Fungal Diversity 101: 113 (2020) Index Fungorum number : IF 805464; Facesoffungi number : FoF 00169; Figs 10 , 11 Saprobic on dead branches of Ficus ampelas and Tamarindus indica . Sexual morph : Ascostromata 145–270 μm diam., 155–290 μm high, solitary or gregarious, semi-immersed, erumpent at maturity, uniloculate, globose to subglobose, ostiolate. Peridium 10–90 μm wide, composed of brown to dark brown cells of textura angularis . Hamathecium composed of 2–5 μm wide, oblong to cylindrical, septate, hyaline pseudoparaphyses. Asci 65–190 × 20–30 μm ( x̄ = 110 × 25 μm, n = 15), bitunicate, fissitunicate, 8-spored, clavate to cylindro-clavate, short pedicellate, straight to slightly curved, apically rounded with well-developed ocular chamber. Ascospores 20–35 × 10–17 μm ( x̄ = 28 × 14 μm, n = 30), 1−2-seriate, overlapping in the ascus, hyaline to yellowish brown when immature, becoming brown to dark brown at maturity, ellipsoid to ovoid, aseptate, with granular appearance, widest at the center and tapering toward narrow ends, straight, small apiculus at either end, smooth-walled. Asexual morph : Undetermined. Culture characteristics :—Ascospores germinating on PDA within 6 hours and germ tube produced from one side of the ascospore. Colonies on PDA reaching 5–7 cm diam. after 3 days at 25 °C, circular, flattened, fluffy, dense, grey in both upper and lower sides. Material examined: — THAILAND . Chiang Mai : Mae Tang Watershed Research Station, on dead branches of Tamarindus indica ( Fabaceae ) , 7 July 2021 , Achala Rathnayaka (MFLU 22-0095, new host record ). TAIWAN . National Chiayi University (NCYU), on dead branch of Ficus ampelas ( Moraceae ) , 21 August 2019 , Dhanushka Tennakoon (MFLU 22-0099, new host and geographical record ); living culture NCYUCC 19-0423. Known hosts and distribution :— Chromolaena odorata , Eucalyptus sp. , Tectona grandis , Tamarindus indica in Thailand (Mapook et al. 2021, Liu et al. 2012 , Phillips et al. 2013 , this study), Ficus ampelas in Taiwan (this study). Notes :—Our collections (MFLU 22-0095 and NCYUCC 19-0423) clustered with Sphaeropsis eucalypticola isolates (MFLUCC 11-0579, MFLUCC 11-0654, MFLUCC 13-0701 and MFLUCC 17-1499) with relatively high 99% ML bootstrap and 1.00 PP support ( Fig. 2 ). Morphologically, our collections show similar characters to the holotype , such as erumpent, globose to subglobose, solitary or gregarious ascostromata. Asci are cylindro-clavate, short pedicellate, and apically rounded with a well-developed ocular chamber. Ascospores are hyaline, becoming yellowish-brown to dark brown at maturity, ellipsoid to ovoid, widest at the center, aseptate, and smooth-walled with small apiculus at either end ( Liu et al. 2012 ). However, the size of asci and ascospores ( x̄ = 110 × 25 μm and x̄ = 28 × 14 μm) are slightly different to the holotype ( x̄ = 106 × 29 μm and x̄ = 30 × 12 μm) ( Liu et al. 2012 ). Based on morpho-molecular evidence, we conclude that our collections represent new host records on Tamarindus indica and Ficus ampelas in Thailand and Taiwan , respectively. Also, NCYUCC 19-0423 represents a new geographical record of S. eucalypticola from Taiwan . Mapook et al. (2021) introduced MFLUCC 17-1499 isolate as Sphaeropsis chromolaenicola , a new species of Sphaeropsis . When comparing the ITS region (ITS1-5.8S-ITS2), no base pair (0%) differences were found between S . chromolaenicola (MFLUCC 17-1499) and other strains of S. eucalypticola (MFLUCC 11-0579, MFLUCC 11-0654, MFLUCC 13-0701, MFLU 22-0095 and NCYUCC 19-0423). According to our phylogenetic analyses, the S . chromolaenicola isolate clusters with other S. eucalypticola isolates ( Fig. 2 ). Therefore, we synonymize S . chromolaenicola under S. eucalypticola .