A new species of Nitokra Boeck, 1865 (Copepoda, Harpacticoida, Ameiridae) from the Caribbean coast of Colombia Author Fuentes-Reines, Juan M. Universidad del Magdalena, Grupo de Investigacion en Biodiversidad y Ecologia Aplicada, A. A 731 Santa Marta, Magdalena, Colombia Author Suarez-Morales, Eduardo https://orcid.org/0000-0003-2051-8707 El Colegio de la Frontera Sur (ECOSUR), A. P. 424, 77014 Chetumal, Quintana Roo, Mexico esuarez@ecosur.mx Author Silva-Briano, Marcelo Departamento de Biologia, Universidad Autonoma de Aguascalientes (UAA), 20131 Aguascalientes, Mexico text ZooKeys 2022 2022-11-08 1128 111 127 http://dx.doi.org/10.3897/zookeys.1128.86210 journal article http://dx.doi.org/10.3897/zookeys.1128.86210 1313-2970-1128-111 2BC60E0BB61948159AD95D4E8507B3A1 F047B33F4FDA542996EF7729F467797E Nitokra puebloviejensis sp. nov. Figs 1 , 2 , 3 , 4 , 5 , 6 Material examined. Adult female holotype ( INV10139 ) , ethanol-preserved, vial, Cienaga Grande de Santa Marta , Colombia (10°5211.25"N, 74°19'31.64"W ), littoral plankton, coll. J.M. Fuentes-Reines ; adult male allotype (INV CRU10140 ) , ethanol-preserved, vial, same sampling data as holotype. Paratypes : four females (INV CRU10141 ), and three males (INV CRU10142 ), same sampling data as holotype and allotype . Additional material. Six adult females, four adult males in first authors' collection. One female and two male individuals prepared for SEM analysis. Type locality. Puebloviejo, Cienaga Grande de Santa Marta, northern Colombia ( 10°52'11.25"N , 74°19'31.64"W ). Etymology. The new species is named in reference to the type locality of the new species by adding the toponimic suffix in singular. The gender of the species suffix is feminine to match that of the genus. Differential diagnosis. Nitokra with 1 inner seta and 5 setae on P1EXP2 and EXP3, respectively and 455 and 777 elements on P2-P4ENP3 and P2-P4EXP3, respectively, plus 111 inner setae on P2-P4ENP1, respectively. Female rostrum hourglass-shaped, distal segment of mandibular palp with 6 setal elements. P1ENP1 almost reaching distal margin of P1EXP3. Modified, club-shaped inner basipodal seta on leg1. Maxilliped with distinctive group of 5 setae inserted medially on the syncoxa. Description of female. Body subcylindrical, tapering posteriorly (Fig. 1A ), total body length 728-784 μm (average = 768 μm , n = 6; holotype length = 784 μm ). Figure 1. Nitokra puebloviejensis sp. nov. from Puebloviejo, Cienaga Grande de Santa Marta, Colombia, digital photos. A holotype female, habitus, lateral view B rostrum, ventral view C labrum, ventral view D urosomites and caudal rami, ventral view E anal somite with anal operculum and spine ornamentation, dorsal view F anal somite and caudal rami showing caudal setae I-VII, ventral view. Scale bars: 50 μm ( A, C ); 10 μm ( B ); 20 μm ( E, F ). Rostrum small, slightly protruding, discernible in dorsal view; roughly hourglass-shaped, with flat tip; rostrum furnished apically with two pairs of short slender sensilla (Figs 1B , 2A ). Labrum subtriangular, strong, heavily chitinized, with rugose edge apically (Figs 1C , 2B ). Urosome short, thick (Figs 1D , 3D-F ), comprising fifth pedigerous somite, genital double-somite and 3 free abdominal somites. Anal somite furnished with row of spinules on proximal ventral surface and along insertion of caudal rami (Figs 1F , 3D-F , 4A ). Anal operculum semicircular, ornamented with 5 or 6 spines (Figs 1E , 4A ). Caudal ramus short, subquadrate, armed with 7 caudal setae (Figs 1F , 4A ), caudal seta I shorter than ramus; seta II about 2.6 x as long as I, seta III on distal outer position, slightly longer than seta II. Setae IV and V thick, long, the former being longest; seta VI about 1.6 x as long as seta I. Dorsal seta (VII) simple, about as long as caudal ramus, shorter than seta VI (Figs 1F , 4A ). Antennule 8-segmented, tapering distally (Fig. 4E ), first segment robust, subquadrate, unornamented, with single seta. Aesthetasc on fourth segment long, reaching beyond distal end of terminal segment. Fourth segment as long as first. Segmental armature as follows (s = seta, ae = aesthetasc): 1(1s),2(4s),3(6s),4(6+1ae).5(3s),6(2),7(4),8(7s+1ae). Antenna (Figs 2E , 3A ). Coxa short, subquadrate, smooth. Basis subrectangular, lacking abexopodal seta, armed with short distal spine and row of minute spinules at spine insertion. First endopodal segment subrectangular, smooth, second endopodal segment longer than first, furnished with subdistal row of spinules on inner margin (Fig. 3A ), with 2 lateral inner spines and 6 apical setal elements, outermost 2 basally fused at insertion. EXP 1-segmented, cylindrical, armed with 3 subequal setal elements (Figs 2E , 3A , 6C, D ). Figure 2. Nitokra puebloviejensis sp. nov. from Puebloviejo, Cienaga Grande de Santa Marta, Colombia. Adult female holotype. A rostrum B labrum C mandible with palp D gnathal blade with teeth and dorsal seta E antenna (some setae cut short) F maxillule showing armature of lobes G maxilla H maxilliped showing row of setal elements on syncoxa I detail of accessory seta of maxilliped ENP J antennule showing segmentation. Scale bars: 15 μm ( A-D, I ); 20 μm ( D-F ); 25 μm ( G, H, J ). Figure 3. Nitokra puebloviejensis sp. nov. from Puebloviejo, Cienaga Grande de Santa Marta, Colombia. SEM-prepared adult female. A antenna showing EXP and ENP B posterior end of anal somite and insertion of caudal rami showing ornamentation, semi-lateral view C fifth leg exopods (at arrow), ventral view D anal somite and caudal rami, ventral view E anal somite, ventral view F urosome, ventral view. Mandible (Fig. 2C, D ). Gnathal blade armed with 13 teeth, 6 large, 7 small, 1 long spinule, and long dorsal seta ornamented with short spinules (Fig. 2D ). Mandibular palp 2-segmented, basal segment short, subquadrate, with short robust seta (Fig. 2C ). Endopodal segment subrectangular, armed with 1 short lateral and 5 apical setae (Fig. 2C ). Maxillule (Fig. 2F ). With large praecoxa; precoxal arthrite (pca in Fig. 2F ) rectangular, unornamented, armed with group of 4 apical and 2 subapical spiniform elements. Coxal endite shorter than precoxal arthrite, armed with 1 curved serrate spiniform element and 2 smooth setae (cxe in Fig. 2F ). Basis (bas in Fig. 2F ) shorter than coxal endite, seemingly with five subequal apical and subapical setae; exopod reduced, represented by 1 seta; endopod (enp in Fig. 2F ) 1-segmented, armed with 2 subequal plumose setae inserted apically (Fig. 2F ). Maxilla (Fig. 2G ). Syncoxa unornamented, with 2 endites, proximalmost armed with short spiniform element and slender modified seta furnished with distal tuft of setules; second endite with 2 slender apical setae. Allobasis produced into strong serrate claw with short, curved adjacent spiniform element and slender seta. ENP 1-segmented, armed with 2 setae. Maxilliped (Fig. 2H, I ). Subchelate. Syncoxa with single setulated seta on inner distal corner and distinctive group of 4 or 5 short slender elements inserted medially on the outer margin of the syncoxa, as in male (Fig. 2H , arrowhead in Fig. 6E ). ENP represented by long, slender claw with short accessory seta (Fig. 2I ). P1 (Fig. 5A ). Intercoxal sclerite smooth. Coxa with transverse row of spinules proximally and spinules row distally. Basis with spinules bordering insertion of exopodal and endopodal rami, inner basipodal spine short, reaching proximal 1/3 of length of first ENP segment. Outer basipodal spine short. EXP and ENP 3-segmented. Exopodal ramus shorter than endopod, reaching about the margin end of second exopodal segment. EXP1lacking inner setae, EXP2 with inner seta; EXP3 with 3 outer spines and 2 geniculate apical setae. ENP 3-segmented; ENP 2 with inner seta, ENP1 subrectangular. ENP3 with 1 apical spiniform seta, 1 geniculate apical seta, and short plumose inner seta. P2 (Fig. 5B-D ). Intercoxal sclerite with transverse rows of long spinules (Fig. 6C ). Coxa with transverse row of long spinules plus 2 rows of minute spinules (Fig. 6D ). Outer basipodal spine short ( "obs" in Fig. 6B ). EXP and ENP 3-segmented. EXP1 lacking inner seta, EXP2 with long, slender inner seta, EXP3 with 3 outer spines, 1 apical and 3 inner setae. ENP shorter than EXP, reaching slightly beyond halfway of EXP3. ENP-3 with 2 inner setae (Fig. 5B ). P3 (Fig. 5E ). Intercoxal sclerite smooth. Coxa and basis as in P2. EXP and ENP 3-segmented. ENP slightly shorter than EXP. ENP as in P2 except for additional inner plumose seta on ENP3. P4 (Fig. 5F ). Intercoxal sclerite, coxa and basis as in P2 and P3. EXP and ENP 3-segmented. ENP shorter than EXP, barely reaching 1/3 of EXP3. EXP as in P3 except for thinner outer spines on EXP3. ENP as in P3. P5 (Fig. 3C at arrow, 4C). EXP and baseoendopod not fused, baseoendopod subtriangular, reaching EXP midlength, segment bearing 5 setae, apical being longest. EXP subquadrate, with spinules row along inner margin; EXP armed with 5 unequally long setae (Fig. 4C ). Figure 4. Nitokra puebloviejensis sp. nov. from Puebloviejo, Cienaga Grande de Santa Marta, Colombia. A adult female anal and preanal urosomites, dorsal view showing anal operculum and caudal setae I-VII B female anal and preanal urosomites, ventral view C female P5 D female genital field and P6 with setae (arrowed) E male geniculated antennule F modified, club-shaped basipodal spine of male P1 G male leg 6 H male P3, anterior view I male P5, ventral view. Scale bars: 25 μm ( A-D, I ); 10 μm ( E-G ); 20 μm ( H ). P6 (Fig. 4D ). Represented by narrow transverse plate with subdistal lobe-like processes marked by a rounded notch. Plate bearing a small seta on each side (Fig. 4D at arrows). Figure 5. Nitokra puebloviejensis sp. nov. from Puebloviejo, Cienaga Grande de Santa Marta, Colombia. Adult female holotype A leg 1 B leg 2 C leg 2 intercoxal sclerite D leg 2 coxa and basipod ornamentation, anterior view E leg3 F leg 4. Scale bars: 20 μm ( A, B, D-F ); 10 μm ( C ). Armature formula of female P1-P5 as follows:

-	Exopod	Endopod
P1	I-0; I-1; III,2,0	0-1;0-1;I,I,2,0
P2	I-0; I-1;III,2,2	0-1;0-1; I,2,1
P3	I-0; I-1;III,2,2	0-1;0-1; I,2,2
P4	I-0;I-1;III,2,2	0-1;0-1;I,2,2
P5	5	5

Male. Smaller than female, total body length 578-588 μm (average length = 578 μm , n = 3; allotype specimen length = 588 μm ). Anal operculum, rostrum, antennae (Fig. 6C, D ), and mouthparts as in female. Sexual dimorphism expressed in the antennule, urosome, P1basis, distal inner seta of P3ENP3, P5, and P6. Figure 6. Nitokra puebloviejensis sp. nov. from Puebloviejo, Cienaga Grande de Santa Marta, Colombia. SEM-prepared adult male individual A antennules, semi-lateral view B same, showing detail of proximal segments C antenna, posterior view D same showing exopodal ramus and endopodal segments E maxilliped, ventral view showing row of setal elements on syncoxal medial surface (arrowhead) F leg 1 showing modified, club-shaped basipodal spine (at arrow). Antennule (Fig. 6A, B ) haplocer, 10-segmented; armature formula difficult to discern, purportedly as follows: 1(1s), 2(9s), 3(6s), 4(2s), 5(9+ae), 6(1s),7(3s), 8(1s),9(2s),10(7+ae). Antenna (Fig. 6C, D ), maxilliped (Fig. 6E ), mouthparts, and P1-P4 as in female. Ventral ornamentation of urosomites as in female except urosomite 2. Posterior margins of urosomites with row of small spinules. P1 basis and P3ENP3. P1 basis with modified, club-shaped inner spine (Fig. 4F , arrowheads in Fig. 6F ). P3ENP3 with distal inner spine thinner than in female. P5 (Fig. 4I ). EXP and baseoendopod separated. The former subquadrate, with 5 or 6 setae, Baseoendopod reaching about proximal 1/3 of EXP, armed with 4 elements. P6. With 2 unequal setae, inner one about 3 x as long as outer seta. Caudal rami as in female. Variability. One male with 3 setal elements (instead of 4) on P5ENP. Another male was observed to possess 6 setae instead of 5 on P5EXP. Habitat. The new species is known only from the type locality, Puebloviejo, Cienaga Grande de Santa Marta (northern Colombia). The site where it was collected is a shallow mangrove area, 0.7 m deep, with water temperature 26-31 °C; local salinity was 15-20 PSU, and pH values was 7.5-8.1.