Adriatic calcarean sponges (Porifera, Calcarea), with the description of six new species and a richness analysis Author Klautau, Michelle C6BB3D65-7166-4A2A-AF2B-7F13EE94F485 Universidade Federal do Rio de Janeiro, Instituto de Biologia, Departamento de Zoologia, Av. Carlos Chagas Filho 373, Cidade Universitária, 21941 - 902 Rio de Janeiro (RJ), Brazil. & urn: lsid: zoobank. org: author: C 6 BB 3 D 65 - 7166 - 4 A 2 A-AF 2 B- 7 F 13 EE 94 F 485 & Corresponding authors: mklautau @ biologia. ufrj. br; mimesek @ irb. hr mklautau@biologia.ufrj.br Author Imešek, Mirna 5461D38C-E1B5-48B1-A41B-0134F337A143 Ruđer Bošković Institute, Division for Molecular Biology, Bijenička cesta 54, 10002 Zagreb, Croatia. & urn: lsid: zoobank. org: author: 5461 D 38 C-E 1 B 5 - 48 B 1 - A 41 B- 0134 F 337 A 143 & Corresponding authors: mklautau @ biologia. ufrj. br; mimesek @ irb. hr mklautau@biologia.ufrj.br Author Azevedo, Fernanda DC0BE6B4-F24F-4765-BA2B-ED17D774B2B7 Universidade Federal do Rio de Janeiro, Instituto de Biologia, Departamento de Zoologia, Av. Carlos Chagas Filho 373, Cidade Universitária, 21941 - 902 Rio de Janeiro (RJ), Brazil. & E-mail: nandaporifera @ gmail. com & urn: lsid: zoobank. org: author: DC 0 BE 6 B 4 - F 24 F- 4765 - BA 2 B-ED 17 D 774 B 2 B 7 Author Pleše, Bruna C9E3BEB1-01E6-44A4-807B-4800E9393C09 Ruđer Bošković Institute, Division for Molecular Biology, Bijenička cesta 54, 10002 Zagreb, Croatia. & E-mail: bplese @ irb. hr & urn: lsid: zoobank. org: author: C 9 E 3 BEB 1 - 01 E 6 - 44 A 4 - 807 B- 4800 E 9393 C 09 Author Nikolić, Vedran 84828CF3-6BA3-4541-AEF2-24422CEA0179 Institute of Oceanography and Fisheries, Laboratory for Benthos, P. O. Box 500, 21000, Split, Croatia. Equally contributed & E-mail: nikolic @ izor. hr & urn: lsid: zoobank. org: author: 84828 CF 3 - 6 BA 3 - 4541 - AEF 2 - 24422 CEA 0179 Author Ćetković, Helena BD5D084E-8AAE-4CBA-991B-0901EB6C8DE1 Ruđer Bošković Institute, Division for Molecular Biology, Bijenička cesta 54, 10002 Zagreb, Croatia. & E-mail: cetkovic @ irb. hr & urn: lsid: zoobank. org: author: BD 5 D 084 E- 8 AAE- 4 CBA- 991 B- 0901 EB 6 C 8 DE 1 text European Journal of Taxonomy 2016 2016-03-02 178 1 52 journal article 22049 10.5852/ejt.2016.178 2fb82473-fc03-4cd0-87ab-4948df6faf23 2118-9773 3832959 E70C7637-C476-46CA-BAA7-BA959E0E64F5 Clathrina conifera Klautau & Borojević, 2001 Fig. 6 ; Table 6 Clathrina conifera Klautau & Borojević, 2001: 404 . Clathrina primordialis ( non C. primordialis ( Haeckel, 1872 )) – Borojević 1971: 527 . — Borojević & Peixinho 1976: 992 . — Mothes de Moraes 1985: 228. — Klautau et al. 1994: 372 . — Muricy & Silva 1999: 160 . Clathrina conifera – Klautau & Valentine 2003: 18 . — Monteiro & Muricy 2004: 682 . — Muricy & Hajdu 2006: 86 . — Lanna et al. 2007: 1554 . — Custódio & Hajdu 2011: 4 . — Muricy et al. 2011: 33 . — Bouzon et al. 2012: 42 . Material examined Holotype BRAZIL : Arraial do Cabo , Rio de Janeiro ( BMNH 1999.9 .16.19, in ethanol). Other material ADRIATIC SEA: near the Island of Lokrum, 42°37’55.6” N , 18°06’49.4” E ; 1–3 m deep, collected by V . Nikolić, 8 Oct. 2010 (PMR-13738 = UFRJPOR 6869); near Dubrovnik, 42°38'26.5" N , 18°06'14.2" E ; 1 m , collected by V . Nikolić, 24 Sep. 2011 (PMR-17807, IRB-S2 = UFRJPOR 7541, IRB-S 3 = UFRJPOR 7542). Colour White in life and white or brown in ethanol. Fig. 6. Clathrina conifera (PMR 13738 = UFRJPOR 6869). A . Specimen in ethanol. B . Tangential section. C . Triactines with variable sizes and shapes. Table 6. Spicule measurements (triactines) of Clathrina conifera Klautau & Borojević, 2001 (PMR- 13738 = UFRJPOR 6869; IRB-S2 = UFRJPOR 7541; IRB-S3 = UFRJPOR 7542).

length (µm)		width (µm)
min	mean	sd	max	min	mean	sd	max	n
PMR-13738 = UFRJPOR6869	57.5	88.5	11.8	122.5	7.5	10.2	1.6	15.0	30
IRB-S2 = UFRJPOR7541	59.4	86.3	9.6	97.2	8.1	9.5	1.4	10.8	20
IRB-S3 = UFRJPOR7542	64.8	82.5	8.9	102.6	6.8	8.8	1.3	10.8	20

Description Cormus composed of irregular and loosely anastomosed tubes ( Fig. 6A ). Water-collecting tubes are not present. The skeleton consists of triactines without organisation ( Fig. 6B ). Spicules ( Table 6 ) TRIACTINES. Regular (equiangular and equiradiate). Their size is very variable. Actines are slightly conical to conical, straight, with blunt tips. Sometimes they are slightly undulated near the tips ( Fig. 6C ). Size: 88.5/ 10.2 µm . Ecology Specimens were collected on a semi-shaded, vertical hard limestone bottom under overhangs. They were often found in association with the macroalga Ellisolandia elongata (J. Ellis & Solander, 1786) . Remarks Until now, this species was considered endemic to Brazil ( Borojević 1971 ; Mothes de Moraes 1985; Klautau et al. 1994 ; Monteiro & Muricy 2004 ; Muricy & Hajdu 2006 ; Lanna et al. 2007 ). Originally, it was identified as C. primordialis ( Haeckel, 1872 ) ( Borojević 1971 ; Mothes de Moraes 1985; Klautau et al. 1994 ). However, considering differences in the size of the actines ( holotype of C. conifera : 62.5–77.3(±9.3)–97.5/9(±1.0) µm; Haeckel measurements of C. primordialis : 100–150/ 8–12 µm ), it was described as a new species: C. conifera . In the present work, we confirmed by morphological and molecular analyses ( Fig. 16 ) that C. conifera is really distinct from C. primordialis and that it occurs in the Adriatic Sea.